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使用敲除细胞株进行验证重组RabMAb

重组Anti-SCRIBBLE抗体[EPR4140(2)] (ab125080)

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Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)
  • Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)
  • Flow Cytometry (Intracellular) - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)
  • Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4140(2)] to SCRIBBLE
  • Suitable for: WB, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-SCRIBBLE抗体[EPR4140(2)]
    参阅全部 SCRIBBLE 一抗

  • 描述

    兔单克隆抗体[EPR4140(2)] to SCRIBBLE

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, Flow Cyt (Intra)more details
    不适用于: IHC-P or IP

  • 种属反应性

    与反应: Human
    不与反应: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human SCRIBBLE aa 1550-1650 (C terminal). The exact sequence is proprietary.

  • 阳性对照

    • 293T, HeLa and MCF7 cell lysates; permeabilized 293T cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • 存储溶液

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR4140(2)

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab125080于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Detects a band of approximately 250 kDa (predicted molecular weight: 175 kDa).
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
说明
WB
1/1000 - 1/10000. Detects a band of approximately 250 kDa (predicted molecular weight: 175 kDa).
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
应用说明
Is unsuitable for IHC-P or IP.

靶标

  • 功能

    Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity.

  • 组织特异性

    Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level).

  • 序列相似性

    Belongs to the LAP (LRR and PDZ) protein family.
    Contains 16 LRR (leucine-rich) repeats.
    Contains 4 PDZ (DHR) domains.

  • 翻译后修饰

    Ubiquitinated; targeted for UBE3A-dependent multiubiquitination in the presence of high-risk HPV E6 proteins and degraded.

  • 细胞定位

    Cell membrane. Cell junction > adherens junction. Cytoplasm. Targeting to cell-cell junctions which is CDH1-dependent is required for the pro-apoptotic activity. Localizes to neuronal post- and pre-synaptic regions.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CRIB 1 antibody
      • CRIB1 antibody
      • hScrib antibody
      • LAP 4 antibody
      • LAP4 antibody
      • PDZ domain protein scribble antibody
      • Protein LAP4 antibody
      • Protein scribble homolog antibody
      • SCRB 1 antibody
      • SCRB1 antibody
      • SCRIB 1 antibody
      • Scrib antibody
      • SCRIB_HUMAN antibody
      • SCRIB1 antibody
      • Scribble antibody
      • Scribble homolog 1 antibody
      • scribbled homolog (Drosophila) antibody
      • Vartul antibody
      see all

    图片

    • Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)
      Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)
      All lanes : Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080) at 1/500 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : SCRIB knockout HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 175 kDa
      Observed band size: 240 kDa why is the actual band size different from the predicted?



      Lanes 1-2: Merged signal (red and green). Green - ab125080 observed at 240 kDa. Red - loading control ab8245 observed at 36 kDa.

       ab125080 Anti-SCRIBBLE antibody [EPR4140(2)]  was shown to specifically react with SCRIBBLE in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265190 (knockout cell lysate ab257660) was used. Wild-type and SCRIBBLE knockout samples were subjected to SDS-PAGE. ab125080 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)
      Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)
      All lanes : Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 175 kDa

    • Flow Cytometry (Intracellular) - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)
      Flow Cytometry (Intracellular) - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)

      ab125080 at 1/100, staining SCRIBBLE in permeabilized 293T cells by intracellular flow cytometry (red), compared with a negative control Rabbit IgG antibody (green).

       

    • Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)
      Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)

    文献 (0)

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    ab125080 尚未被引用在任何文献中。

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