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Anti-SCNN1B (phospho T615)抗体(ab79172)

Submit a review Q&A (2)References (1)
Western blot - Anti-SCNN1B (phospho T615) antibody (ab79172)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCNN1B (phospho T615) antibody (ab79172)
  • Immunocytochemistry/ Immunofluorescence - Anti-SCNN1B (phospho T615) antibody (ab79172)

Key features and details

  • Rabbit polyclonal to SCNN1B (phospho T615)
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human, African green monkey
  • Isotype: IgG

概述

  • 产品名称

    Anti-SCNN1B (phospho T615)抗体
    参阅全部 SCNN1B 一抗

  • 描述

    兔多克隆抗体to SCNN1B (phospho T615)

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-P, ICC/IFmore details

  • 种属反应性

    与反应: Human, African green monkey
    预测可用于: Mouse, Rat

  • 免疫原

    Synthesized phosphopeptide derived from human SCNN1B around the phosphorylation site of threonine 615 (P-G-TP-P-P).

  • 阳性对照

    • Human brain tissue; extracts from HeLa cells; COS7 cells.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab79172于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/500 - 1/1000. Predicted molecular weight: 73 kDa.
IHC-P
1/50 - 1/100.
ICC/IF
1/500 - 1/1000.
说明
WB
1/500 - 1/1000. Predicted molecular weight: 73 kDa.
IHC-P
1/50 - 1/100.
ICC/IF
1/500 - 1/1000.

靶标

  • 功能

    Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.

  • 疾病相关

    Pseudohypoaldosteronism 1, autosomal recessive
    Liddle syndrome
    Bronchiectasis with or without elevated sweat chloride 1

  • 序列相似性

    Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1B subfamily.

  • 翻译后修饰

    Phosphorylated on serine and threonine residues.

  • 细胞定位

    Apical cell membrane. Apical membrane of epithelial cells.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Amiloride sensitive sodium channel subunit beta antibody
    • Amiloride-sensitive sodium channel subunit beta antibody
    • Beta NaCH antibody
    • Beta-ENaC antibody
    • Beta-NaCH antibody
    • ENaC beta antibody
    • ENaCB antibody
    • Epithelial Na(+) channel subunit beta antibody
    • Epithelial Na+ channel beta subunit antibody
    • Epithelial Na+ channel subunit beta antibody
    • Epithelial sodium channel beta 2 subunit antibody
    • Epithelial sodium channel beta 3 subunit antibody
    • Nonvoltage gated sodium channel 1 beta subunit antibody
    • Nonvoltage gated sodium channel 1 subunit beta antibody
    • Nonvoltage-gated sodium channel 1 subunit beta antibody
    • SCNEB antibody
    • SCNN 1B antibody
    • SCNN1B antibody
    • SCNNB_HUMAN antibody
    • Sodium channel nonvoltage gated 1 beta (Liddle syndrome) antibody
    • Sodium channel nonvoltage gated 1 beta antibody
    see all

图片

  • Western blot - Anti-SCNN1B (phospho T615) antibody (ab79172)
    Western blot - Anti-SCNN1B (phospho T615) antibody (ab79172)
    All lanes : Anti-SCNN1B (phospho T615) antibody (ab79172) at 1/500 dilution

    Lane 1 : extracts from HeLa cells
    Lane 2 : extracts from HeLa cells with immunizing phosphopeptide at 10 µg

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 73 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCNN1B (phospho T615) antibody (ab79172)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCNN1B (phospho T615) antibody (ab79172)
    Immunohistochemiscal analysis of SCNN1B in paraffin embedded human brain tissue using a 1/50 dilution of ab79172 in the presence (right) or absence (left) of the immunizing phosphopeptide.
  • Immunocytochemistry/ Immunofluorescence - Anti-SCNN1B (phospho T615) antibody (ab79172)
    Immunocytochemistry/ Immunofluorescence - Anti-SCNN1B (phospho T615) antibody (ab79172)
    Immunofluorescence analysis of SCNN1B in COS7 cells, using a 1/500 dilution of ab79172, in the presence (right) or absence (left) of the immunizing phosphopeptide.

文献 (1)

发表研究结果有使用 ab79172？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab79172 被引用在 1 文献中.

  • Shimko MJ  et al. Nerve growth factor reduces amiloride-sensitive Na+ transport in human airway epithelial cells. Physiol Rep 2:N/A (2014). WB ; Human . PubMed: 25347857

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Question

Is the immunizing peptide for ab79172 available?

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Answer

I have contacted the lab and they have told me that the immunogen sequence for ab79172 is the sequence between *******of the human sequence. If there is anything else I can help you with, please let me know.

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Question

Is the immunizing peptide for ab79172 available?

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Answer

Thank you for contacting Abcam. I have heard back from the lab and they have told me that we currently do not have enough of the immunizing peptide used to generate ab79172 to sell. I am sorry that I could not provide you with the immunizing peptide, but it if there is anything else I can help you with, please let me know.

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