Anti-SCN3A抗体(ab65164)
Key features and details
- Rabbit polyclonal to SCN3A
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SCN3A抗体
参阅全部 SCN3A 一抗 -
描述
兔多克隆抗体to SCN3A -
宿主
Rabbit -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human SCN3A aa 80-130.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: Whole serum -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab65164于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/1000.
|
| 说明 |
|---|
|
ICC/IF
1/1000. |
靶标
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功能
Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. -
序列相似性
Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.3/SCN3A subfamily.
Contains 1 IQ domain. -
结构域
The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position. -
翻译后修饰
May be ubiquitinated by NEDD4L; which would promote its endocytosis.
Phosphorylation at Ser-1501 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6328 Human
- Entrez Gene: 20269 Mouse
- Entrez Gene: 497770 Rat
- Omim: 182391 Human
- SwissProt: Q9NY46 Human
- SwissProt: P08104 Rat
- Unigene: 435274 Human
- Unigene: 87394 Rat
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别名
- brain III voltage-gated sodium channel antibody
- NAC3 antibody
- Nav1.3 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-SCN3A antibody (ab65164)ICC/IF image of ab65164 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65164, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
数据表及文件
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Datasheet download
文献 (3)
ab65164 被引用在 3 文献中.
- Pintacuda G et al. Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders. Cell Genom 3:100250 (2023). PubMed: 36950384
- Wang Z et al. Voltage-Gated Sodium Channels Are Involved in Cognitive Impairments in Parkinson's Disease- like Rats. Neuroscience 418:231-243 (2019). PubMed: 31473280
- Wang Z et al. Re-expression of voltage-gated sodium channel subtype Nav1.3 in the substantia nigra after dopamine depletion. Neurosci Lett 687:146-152 (2018). PubMed: 30267849
