重组Anti-Sclerostin抗体[EPR23973-8] - BSA and Azide free (Capture) (ab281182)
![Sandwich ELISA - Anti-Sclerostin antibody [EPR23973-8] - BSA and Azide free (Capture) (ab281182)](https://www.abcam.cn/ps/products/281/ab281182/Images/ab281182-2-mouse-sost-elisa-kit-sandwich-elisa-standard-curve-4.jpg "Sandwich ELISA - Anti-Sclerostin antibody [EPR23973-8] - BSA and Azide free (Capture) (ab281182)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23973-8] to Sclerostin - BSA and Azide free (Capture)
- Suitable for: Sandwich ELISA
- Reacts with: Mouse
概述
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产品名称
Anti-Sclerostin抗体[EPR23973-8] - BSA and Azide free (Capture)
参阅全部 Sclerostin 一抗 -
描述
兔单克隆抗体[EPR23973-8] to Sclerostin - BSA and Azide free (Capture) -
宿主
Rabbit -
经测试应用
适用于: Sandwich ELISAmore details -
种属反应性
与反应: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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常规说明
ab281182 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse Sost. The recommended pair for sandwich ELISA is:
Capture: ab281182, Mouse Sost Capture Antibody (unconjugated)
Detector: ab281032, Mouse Sost Detector Antibody (unconjugated)
The reference range value is 15.6 - 1000 pg/mL.Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Constituent: 100% PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23973-8 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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sELISA pair antibody
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab281182于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR23973-1] to Sclerostin - BSA and Azide free (Detector) (ab281032).
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR23973-1] to Sclerostin - BSA and Azide free (Detector) (ab281032). |
靶标
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功能
Negative regulator of bone growth. -
组织特异性
Widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteeoblasts differentiated for 21 days. -
疾病相关
Defects in SOST are the cause of sclerosteosis (SOST) [MIM:269500]; also known as cortical hyperostosis with syndactyly. SOST is an autosomal recessive sclerosing bone dysplasia characterized by a generalized hyperostosis and sclerosis leading to a markedly thickened skull, with mandible, ribs, clavicles and all long bones also being affected. Due to narrowing of the foramina of the cranial nerves, facial nerve palsy, hearing loss and atrophy of the optic nerves can occur. Sclerosteosis is clinically and radiologically very similar to van Buchem disease, mainly differentiated by hand malformations and a large stature in sclerosteosis patients.
Note=A 52 kb deletion downstream of SOST results in SOST transcription suppression and is a cause of van Buchem disease (VBCH) [MIM:239100]; also known as hyperostosis corticalis generalisata. VBCH is an autosomal recessive sclerosing bone dysplasia characterized by endosteal hyperostosis of the mandible, skull, ribs, clavicles, and diaphyses of the long bones. Affected patients present a symmetrically increased thickness of bones, most frequently found as an enlarged jawbone, but also an enlargement of the skull, ribs, diaphysis of long bones, as well as tubular bones of hands and feet. The clinical consequence of increased thickness of the skull include facial nerve palsy causing hearing loss, visual problems, neurological pain, and, very rarely, blindness as a consequence of optic atrophy. Serum alkaline phosphatase levels are elevated. -
序列相似性
Belongs to the sclerostin family.
Contains 1 CTCK (C-terminal cystine knot-like) domain. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 74499 Mouse
- SwissProt: Q99P68 Mouse
- Unigene: 265602 Mouse
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别名
- BEER antibody
- CDD antibody
- Cortical hyperostosis with syndactyly antibody
see all
图片
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Sandwich ELISA - Anti-Sclerostin antibody [EPR23973-8] - BSA and Azide free (Capture) (ab281182)Example of Mouse SOST standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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![Anti-Sclerostin antibody [EPR23973-8] - BSA and Azide free (Capture) (ab281182)](https://www.abcam.cn/ps/products/281/ab281182/Images/ab281182-1-RecombinantAntibodyBenefitfigure.png)
Anti-Sclerostin antibody [EPR23973-8] - BSA and Azide free (Capture) (ab281182)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab281182 尚未被引用在任何文献中。
