Anti-SCGN/Secretagogin抗体(ab111871)
Key features and details
- Rabbit polyclonal to SCGN/Secretagogin
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-SCGN/Secretagogin抗体
参阅全部 SCGN/Secretagogin 一抗 -
描述
兔多克隆抗体to SCGN/Secretagogin -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow
-
免疫原
Recombinant fragment corresponding to Human SCGN/Secretagogin aa 57-238. (AAH00336)
-
阳性对照
- HeLa and HCT116 cells and whole cell lysates.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab111871 is purified by antigen-affinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab111871于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 32 kDa.
|
|
| ICC/IF |
1/100 - 1/500.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 32 kDa. |
|
ICC/IF
1/100 - 1/500. |
靶标
-
组织特异性
Expressed at high levels in the pancreatic islets of Langerhans and to a much lesser extent in the gastrointestinal tract (stomach, small intestine and colon), the adrenal medulla and cortex and the thyroid C-cells. In the brain, the expression is restricted to distinct subtypes of neurons with highest expression in the molecular layer of the cerebellum (stellate and basket cells), in the anterior part of the pituitary gland, in the thalamus, in the hypothalamus and in a subgroup of neocortical neurons. -
序列相似性
Contains 6 EF-hand domains. -
细胞定位
Cytoplasm. Secreted. Cytoplasmic vesicle > secretory vesicle membrane. Predominantly cytoplasmic. A small proportion is associated with secretory granules and membrane fractions (By similarity). Detectable in human serum after ischemic neuronal damage. - Information by UniProt
-
数据库链接
- Entrez Gene: 618206 Cow
- Entrez Gene: 10590 Human
- Entrez Gene: 214189 Mouse
- Entrez Gene: 306942 Rat
- Omim: 609202 Human
- SwissProt: A5PJN0 Cow
- SwissProt: O76038 Human
- SwissProt: Q91WD9 Mouse
see all -
别名
- Calbindin like antibody
- CALBL antibody
- DJ501N12.8 antibody
see all
图片
-
Western blot - Anti-SCGN/Secretagogin antibody (ab111871)Anti-SCGN/Secretagogin antibody (ab111871) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 32 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-SCGN/Secretagogin antibody (ab111871)ab111871 at 1/200 dilution staining SCGN/Secretagogin in methanol-fixed HeLa cells by Immunofluorescence. The lower image is costained with Hoechst 33342.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab111871 被引用在 3 文献中.
- Shi Y et al. Mouse and human share conserved transcriptional programs for interneuron development. Science 374:eabj6641 (2021). PubMed: 34882453
- Garas FN et al. Structural and molecular heterogeneity of calretinin-expressing interneurons in the rodent and primate striatum. J Comp Neurol 526:877-898 (2018). Mouse . PubMed: 29218729
- Garas FN et al. Secretagogin expression delineates functionally-specialized populations of striatal parvalbumin-containing interneurons. Elife 5:N/A (2016). PubMed: 27669410