AB138493

重组Anti-SCGN/Secretagogin抗体[EPR7476]

Anti-SCGN/Secretagogin antibody [EPR7476]

RabMAb
Recombinant
了解详情

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(3 Publications)

Rabbit Recombinant Monoclonal SCGN/Secretagogin antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 3 publications.

查看别名

SECRET, SCGN, Secretagogin

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7476] (AB138493)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7476] (AB138493)

Immunohistochemical analysis of paraffin-embedded Human gastric neuroendocrine carcinoma tissue labelling SCGN/Secretagogin with ab138493 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCGN/Secretagogin antibody [EPR7476] (AB138493)

Immunohistochemical analysis of paraffin-embedded Human pancreas carcinoma tissue labelling SCGN/Secretagogin with ab138493 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-SCGN/Secretagogin antibody [EPR7476] (AB138493)

All lanes:

Western blot - Anti-SCGN/Secretagogin antibody [EPR7476] (ab138493) at 1/1000 dilution

Lane 1:

Fetal brain lysate at 10 µg

Lane 2:

Fetal midbrain lysate at 10 µg

Lane 3:

C6 lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 32 kDa

false

OI-RD Scanning - Anti-SCGN/Secretagogin antibody [EPR7476] (AB138493)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SCGN/Secretagogin antibody [EPR7476] (AB138493)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

不同偶联物与剂型 (1)

Carrier free

Anti-SCGN/Secretagogin antibody [EPR7476] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR7476

亚型

IgG

不含载体蛋白

反应种属

Rat, Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SCGN also known as Secretagogin is a calcium-binding protein with a molecular weight of approximately 33 kDa. It belongs to the family of EF-hand superfamily proteins which is known for its role in various cellular processes. Secretagogin predominantly expresses in neuroendocrine tissues such as the pancreas and central nervous system where it binds calcium and possibly modulates its signaling. It has drawn interest due to its distinctive expression pattern and potential role in regulated secretion.

Biological function summary

The role of Secretagogin extends beyond simple calcium binding. It participates in managing cytoskeleton dynamics through its ability to bind calcium impacting processes like vesicle trafficking. Although not part of a standard protein complex Secretagogin interacts with other cytoskeletal proteins and may influence the release of hormones. Its role in neurotransmission and insulin secretion showcases its significance in both the nervous and endocrine systems.

Pathways

Secretagogin interacts within signaling networks such as the calcium-signaling and insulin-secretion pathways. It has established relationships with other proteins like actin influencing cellular architecture and trafficking. Through these pathways Secretagogin appears instrumental in processes involving rapid response times such as neurotransmitter release or hormone secretion often acting in conjunction with or supporting other calcium-binding proteins.

Secretagogin has connections to diabetes and certain neuroendocrine tumors. Its involvement in insulin secretion makes it relevant in the context of diabetes where it might interact with malfunctioning insulin-related proteins. Additionally in neuroendocrine tumors altered Secretagogin expression could be linked with oncogenic processes as it shares pathways with proteins involved in cell-cycle regulation. Understanding these associations can aid in identifying potential therapeutic targets or biomarkers for these diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information SCGN

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1387 PubMed41028816

2025

Uncovering a fibroblast differentiation-based keloid classification by integration of single-cell and bulk RNA sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhang,Jianyu Lu,Xirui Tong,Sujie Xie,Shuyuan Xian,Yifan Liu,Jiale Yan,Weijin Qian,Hanlin Sun,Yushu Zhu,Luofeng Jiang,Xinya Guo,Xinran Ding,Yixu Li,Chenguang Bai,Runzhi Huang,Zhaofan Xia,Shizhao Ji

BMC cancer 25:57 PubMed39794740

2025

Integrative machine learning frameworks to uncover specific protein signature in neuroendocrine cervical carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Shen,Tingting Dong,Haiyang Wang,Yi Ding,Jianuo Zhang,Xinyi Zhu,Yeping Ding,Wen Cai,Yalan Wei,Qiao Wang,Sufen Wang,Feiyun Jiang,Bin Tang

Journal of applied microbiology 132:1370-1383 PubMed34470077

2021

Sodium butyrate alleviates pre-eclampsia in pregnant rats by improving the gut microbiota and short-chain fatty acid metabolites production.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Yong,Yanhua Zhao,Xiao'e Jiang,Ping Li

View all publications

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重组Anti-SCGN/Secretagogin抗体[EPR7476]