重组Anti-SCDGFB/PDGF-D抗体[EPR7266(2)] (ab181845)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7266(2)] to SCDGFB/PDGF-D
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-SCDGFB/PDGF-D抗体[EPR7266(2)]
参阅全部 SCDGFB/PDGF-D 一抗 -
描述
兔单克隆抗体[EPR7266(2)] to SCDGFB/PDGF-D -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human fetal kidney, fetal heart and small intestine lysate. PC-3 cell lysate. Human squamous cell lung carcinoma tissue. Mouse heart tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7266(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181845于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/2000. Predicted molecular weight: 43 kDa.
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IHC-P | (1) |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/2000. Predicted molecular weight: 43 kDa. |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound. Activated by proteolytic cleavage and this active form acts as a specific ligand for beta platelet-derived growth factor receptor. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. -
组织特异性
Expressed at high levels in the heart, pancreas, adrenal gland and ovary and at low levels in placenta, liver, kidney, prostate, testis, small intestine, spleen and colon. In the kidney, expressed by the visceral epithelial cells of the glomeruli. A widespread expression is also seen in the medial smooth muscle cells of arteries and arterioles, as well as in smooth muscle cells of vasa rectae in the medullary area. Expressed in the adventitial connective tissue surrounding the suprarenal artery. In chronic obstructive nephropathy, a persistent expression is seen in glomerular visceral epithelial cells and vascular smooth muscle cells, as well as de novo expression by periglomerular interstitial cells and by some neointimal cells of arteriosclerotic vessels. Expression in normal prostate is seen preferentially in the mesenchyme of the gland while expression is increased and more profuse in prostate carcinoma. Expressed in many ovarian, lung, renal and brain cancer-derived cell lines. -
序列相似性
Belongs to the PDGF/VEGF growth factor family.
Contains 1 CUB domain. -
发展阶段
Not detectable in the earliest stages of glomerulogenesis, and not detected in the metanephric blastema or surrounding cortical interstitial cells. In later stages of glomerulogenesis, localized to epithelial cells transitioning from the early developing nephrons of the comma- and S-shaped stages to the visceral epithelial cells of differentiated glomeruli. In the developing pelvis, expressed at the basement membrane of immature collecting ducts and by presumptive fibroblastic cells in the interstitium. -
翻译后修饰
Proteolytic removal of the N-terminal CUB domain releasing the core domain is necessary for unmasking the receptor-binding epitopes of the core domain. Cleavage after Arg-247 or Arg-249 by urokinase plasminogen activator gives rise to the active form. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 80310 Human
- Entrez Gene: 71785 Mouse
- Entrez Gene: 66018 Rat
- Omim: 609673 Human
- SwissProt: Q9GZP0 Human
- SwissProt: Q925I7 Mouse
- SwissProt: Q9EQT1 Rat
- Unigene: 352298 Human
see all -
别名
- IEGF antibody
- Iris expressed growth factor antibody
- Iris-expressed growth factor antibody
see all
图片
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All lanes : Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human small intestine lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution (Goat Anti-Rabbit IgG HRP)
Predicted band size: 43 kDa -
Immunohistochemical analysis of paraffin-embedded Mouse heart tissue labeling SCDGFB/PDGF-D using ab181845 at 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG prediluted was used as secondary. Counterstain: Hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human squamous cell lung carcinoma tissue labeling SCDGFB/PDGF-D using ab181845 at 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG prediluted was used as secondary. Counterstain: Hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845) at 1/1000 dilution + PC3 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 43 kDa
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (4)
ab181845 被引用在 4 文献中.
- Ma S et al. PDGF-D-PDGFRβ signaling enhances IL-15-mediated human natural killer cell survival. Proc Natl Acad Sci U S A 119:N/A (2022). PubMed: 35027451
- Xu Z et al. Acetylation of Checkpoint suppressor 1 enhances its stability and promotes the progression of triple-negative breast cancer. Cell Death Discov 8:474 (2022). PubMed: 36450706
- Li X et al. Whole-genome resequencing of wild and domestic sheep identifies genes associated with morphological and agronomic traits. Nat Commun 11:2815 (2020). PubMed: 32499537
- Jiang B et al. Platelet-derived growth factor-D promotes colorectal cancer cell migration, invasion and proliferation by regulating Notch1 and matrix metalloproteinase-9. Oncol Lett 15:1573-1579 (2018). PubMed: 29434852