重组Anti-SCD1抗体[EPR21963] (ab236868)
![Western blot - Anti-SCD1 antibody [EPR21963] (ab236868)](https://www.abcam.cn/ps/products/236/ab236868/Images/ab236868-391343-anti-scd1-antibody-epr21963-western-blot-human-knockout-lysate.jpg "Western blot - Anti-SCD1 antibody [EPR21963] (ab236868)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21963] to SCD1
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-SCD1抗体[EPR21963]
参阅全部 SCD1 一抗 -
描述
兔单克隆抗体[EPR21963] to SCD1 -
宿主
Rabbit -
特异性
ab236868 is recommended for human only in WB. -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HepG2, HEK-293 and SK-MEL-28 whole cell lysate. IHC-P: Human adipose tissue within cardiac muscle tissue; Mouse adipose tissue of stomach tissue; Rat adipose tissue of pancreas tissue. ICC/IF: HepG2 and SK-MEL-28 cells. Flow: HepG2 cells. IP: HepG2 and HEK-293 whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR21963 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab236868于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
|
|
| WB |
1/1000. Predicted molecular weight: 42 kDa.
ab236868 is recommended for human only in WB. |
|
| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| ICC/IF |
1/100.
|
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| IP |
1/30.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/500. |
|
WB
1/1000. Predicted molecular weight: 42 kDa. ab236868 is recommended for human only in WB. |
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC/IF
1/100. |
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IP
1/30. |
靶标
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功能
Terminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. -
序列相似性
Belongs to the fatty acid desaturase family. -
结构域
The histidine box domains may contain the active site and/or be involved in metal ion binding. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6319 Human
- Entrez Gene: 20249 Mouse
- Entrez Gene: 83792 Rat
- Omim: 604031 Human
- SwissProt: O00767 Human
- SwissProt: P13516 Mouse
- SwissProt: P07308 Rat
- Unigene: 558396 Human
see all -
别名
- ACOD_HUMAN antibody
- Acyl-CoA desaturase antibody
- Delta(9)-desaturase antibody
see all
图片
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Western blot - Anti-SCD1 antibody [EPR21963] (ab236868)All lanes : Anti-SCD1 antibody [EPR21963] (ab236868) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SCD knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 42 kDaanes 1-3: Merged signal (red and green). Green - ab236868. Red - loading control ab8245 observed at 50 kDa.
ab236868 Anti-SCD1 antibody [EPR21963] was shown to specifically react with SCD1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265220 (knockout cell lysate ab257658) was used. Wild-type and SCD1 knockout samples were subjected to SDS-PAGE. ab236868 and Anti-tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-SCD1 antibody [EPR21963] (ab236868)](https://www.abcam.cn/ps/products/236/ab236868/Images/ab236868-12-rabbit-monoclonal-epr21963-to-scd1-immunocytochemistry.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SCD1 antibody [EPR21963] (ab236868)ab236868 staining SCD1 in wild-type HeLa cells, with negative expression in SCD1 knockout HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab236868 at 0.04 µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin at 0.5 µg/ml. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150119, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647), pre-adsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
This product also work with 100% methanol (5 min) fixation under the same testing conditions.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCD1 antibody [EPR21963] (ab236868)](https://www.abcam.cn/ps/products/236/ab236868/Images/ab236868-315946-anti-scd1-antibody-epr21963-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCD1 antibody [EPR21963] (ab236868)Immunohistochemical analysis of paraffin-embedded human adipose tissue within cardiac muscle tissue labeling SCD1 with ab236868 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in human adipose cells in cardiac muscle (PMID: 15907797) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Western blot - Anti-SCD1 antibody [EPR21963] (ab236868)](https://www.abcam.cn/ps/products/236/ab236868/Images/ab236868-315943-anti-scd1-antibody-epr21963-western-blot.jpg)
Western blot - Anti-SCD1 antibody [EPR21963] (ab236868)All lanes : Anti-SCD1 antibody [EPR21963] (ab236868) at 1/1000 dilution
Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 2 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
Lane 3 : SK-MEL-28 (human malignant melanoma), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 42 kDa
Exposure time: 4 secondsBlocking/dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 20876744; PMID: 9843580; PMID: 17449569). The full-length protein migrates at 37 kDa; the 28 kDa fragment may represent an SCD1 cleavage product.
We recommend that customers do not boil samples to prevent protein aggregation.
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![Immunoprecipitation - Anti-SCD1 antibody [EPR21963] (ab236868)](https://www.abcam.cn/ps/products/236/ab236868/Images/ab236868-315951-anti-scd1-antibody-epr21963-immunoprecipitation.jpg)
Immunoprecipitation - Anti-SCD1 antibody [EPR21963] (ab236868)SCD1 was immunoprecipitated from 0.35 mg of HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate with ab236868 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab236868 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
Lane 1: HEK-293 whole cell lysate 10 μg (input).
Lane 2: ab236868 IP in HEK-293 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236868 in HEK-293 whole cell lysate (-).Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 30 seconds.
The expression profile observed is consistent with what has been described in the literature (PMID: 20876744; PMID: 9843580; PMID: 17449569). The full-length protein migrates at 37 kDa; the 28 kDa fragment may represent an SCD1 cleavage product.
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![Flow Cytometry (Intracellular) - Anti-SCD1 antibody [EPR21963] (ab236868)](https://www.abcam.cn/ps/products/236/ab236868/Images/ab236868-315949-anti-scd1-antibody-epr21963-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SCD1 antibody [EPR21963] (ab236868)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cell line labeling SCD1 with ab236868 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-SCD1 antibody [EPR21963] (ab236868)](https://www.abcam.cn/ps/products/236/ab236868/Images/ab236868-315944-anti-scd1-antibody-epr21963-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SCD1 antibody [EPR21963] (ab236868)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling SCD1 with ab236868 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HepG2 cell line. Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution. Nuclear counterstain is DAPI.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCD1 antibody [EPR21963] (ab236868)](https://www.abcam.cn/ps/products/236/ab236868/Images/ab236868-315948-anti-scd1-antibody-epr21963-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCD1 antibody [EPR21963] (ab236868)Immunohistochemical analysis of paraffin-embedded rat adipose tissue of pancreas tissue labeling SCD1 with ab236868 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in adipose cells in rat pancreas (PMID: 11500518) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCD1 antibody [EPR21963] (ab236868)](https://www.abcam.cn/ps/products/236/ab236868/Images/ab236868-315947-anti-scd1-antibody-epr21963-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCD1 antibody [EPR21963] (ab236868)Immunohistochemical analysis of paraffin-embedded mouse adipose tissue of stomach tissue labeling SCD1 with ab236868 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in adipose cells in mouse stomach (PMID: 11500518) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunoprecipitation - Anti-SCD1 antibody [EPR21963] (ab236868)](https://www.abcam.cn/ps/products/236/ab236868/Images/ab236868-315950-anti-scd1-antibody-epr21963-immunoprecipitation.jpg)
Immunoprecipitation - Anti-SCD1 antibody [EPR21963] (ab236868)SCD1 was immunoprecipitated from 10 μg of HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate with ab236868 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab236868 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
Lane 1: HepG2 whole cell lysate 10 μg (input).
Lane 2: ab236868 IP in HepG2 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236868 in HepG2 whole cell lysate (-).Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 30 seconds.
The expression profile observed is consistent with what has been described in the literature (PMID: 20876744; PMID: 9843580; PMID: 17449569). The full-length protein migrates at 37 kDa; the 28 kDa fragment may represent an SCD1 cleavage product.
-
![Immunocytochemistry/ Immunofluorescence - Anti-SCD1 antibody [EPR21963] (ab236868)](https://www.abcam.cn/ps/products/236/ab236868/Images/ab236868-315945-anti-scd1-antibody-epr21963-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SCD1 antibody [EPR21963] (ab236868)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-MEL-28 (Human malignant melanoma) cells labeling SCD1 with ab236868 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in SK-MEL-28 cell line. Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution. Nuclear counterstain is DAPI.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
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![Anti-SCD1 antibody [EPR21963] (ab236868)](https://www.abcam.cn/ps/products/236/ab236868/Images/ab236868-11-benefits-of-recombinant-antibodies.png)
Anti-SCD1 antibody [EPR21963] (ab236868)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (13)
ab236868 被引用在 13 文献中.
- Sun R et al. Pink Lotus Essential Oil and Alleviates on Free Fatty Acid Induced Steatosis in HepG2 Cells via PI3K/Akt and NF-κB Pathways. J Oleo Sci 71:95-104 (2022). PubMed: 35013040
- Zhang T et al. Dysregulated lipid metabolism blunts the sensitivity of cancer cells to EZH2 inhibitor. EBioMedicine 77:103872 (2022). PubMed: 35158113
- Xiang J et al. LncRNA MALAT1 Promotes PPARα/CD36-Mediated Hepatic Lipogenesis in Nonalcoholic Fatty Liver Disease by Modulating miR-206/ARNT Axis. Front Bioeng Biotechnol 10:858558 (2022). PubMed: 35769097
- Li R et al. Reducing VEGFB accelerates NAFLD and insulin resistance in mice via inhibiting AMPK signaling pathway. J Transl Med 20:341 (2022). PubMed: 35907871
- Wang X et al. The downstream PPARγ target LRRC1 participates in early stage adipocytic differentiation. Mol Cell Biochem N/A:N/A (2022). PubMed: 36370237