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Anti-SCAP抗体(ab125186)

Reviews (3)Q&A (3)References (5)
Western blot - Anti-SCAP antibody (ab125186)
  • Immunoprecipitation - Anti-SCAP antibody (ab125186)

Key features and details

  • Rabbit polyclonal to SCAP
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-SCAP抗体
    参阅全部 SCAP 一抗

  • 描述

    兔多克隆抗体to SCAP

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IPmore details

  • 种属反应性

    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Orangutan

  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 450-500 of Human SCAP (NP_036367.2).

  • 阳性对照

    • HeLa, Jurkat and 293T whole cell lysates

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab125186于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (2)
1/2000 - 1/10000. Predicted molecular weight: 140 kDa.
IP (1)
Use at 2-10 µg/mg of lysate.
说明
WB
1/2000 - 1/10000. Predicted molecular weight: 140 kDa.
IP
Use at 2-10 µg/mg of lysate.

靶标

  • 功能

    Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway.

  • 序列相似性

    Belongs to the WD repeat SCAP family.
    Contains 1 SSD (sterol-sensing) domain.
    Contains 7 WD repeats.

  • 结构域

    Cholesterol bound to SSC domain of SCAP or oxysterol bound to INSIG1/2 leads to masking of an ER export signal on SCAP possibly by moving the signal further away from the ER membrane.

  • 细胞定位

    Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
  • Information by UniProt

  • 数据库链接

    • 别名

      • KIAA0199 antibody
      • SCAP antibody
      • SCAP_HUMAN antibody
      • SREBF chaperone antibody
      • SREBF chaperone protein antibody
      • SREBP cleavage activating protein antibody
      • SREBP cleavage-activating protein antibody
      • Sterol regulatory element binding protein cleavage-activating protein antibody
      • Sterol regulatory element-binding protein cleavage-activating protein antibody
      see all

    图片

    • Western blot - Anti-SCAP antibody (ab125186)
      Western blot - Anti-SCAP antibody (ab125186)
      All lanes : Anti-SCAP antibody (ab125186) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : 293T whole cell lysate at 50 µg
      Lane 4 : Jurkat whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 140 kDa


      Exposure time: 30 seconds
    • Immunoprecipitation - Anti-SCAP antibody (ab125186)
      Immunoprecipitation - Anti-SCAP antibody (ab125186)
      ab125186, at 6 µg/mg lysate, detecting SCAP in HeLa cell lysate (1 mg of immunoprecipitate, 20% loaded in the lane) by Immunoprecipitation.
      For the subsequent Western blot, ab125186 was used at 1 µg/ml.
      The blot was developed using the ECL technique, with an exposure time of 30 seconds.

    文献 (5)

    发表研究结果有使用 ab125186？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab125186 被引用在 5 文献中.

    • Wong W & Sari Y Effects of Chronic Hydrocodone Exposure and Ceftriaxone on the Expression of Astrocytic Glutamate Transporters in Mesocorticolimbic Brain Regions of C57/BL Mice. Toxics 11:N/A (2023). PubMed: 37888720
    • Zhong S  et al. Acetaldehyde Dehydrogenase 2 regulates HMG-CoA reductase stability and cholesterol synthesis in the liver. Redox Biol 41:101919 (2021). PubMed: 33740503
    • Dong G  et al. SCAP Mediated GDF15-Induced Invasion and EMT of Esophageal Cancer. Front Oncol 10:564785 (2020). PubMed: 33123476
    • Pagano S  et al. Anti-ApoA-1 IgGs in Familial Hypercholesterolemia Display Paradoxical Associations with Lipid Profile and Promote Foam Cell Formation. J Clin Med 8:N/A (2019). PubMed: 31766415
    • Benetti E  et al. Effects of vitamin D on insulin resistance and myosteatosis in diet-induced obese mice. PLoS One 13:e0189707 (2018). PubMed: 29342166

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-SCAP antibody

     Poor
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse embryo fibroblasts)
    Gel Running Conditions
    Non-reduced Denaturing
    Loading amount
    50 µg
    Treatment
    RNAi
    Specification
    mouse embryo fibroblasts
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Boyan Zhang

    Verified customer

    提交于 Aug 30 2018

    Immunoprecipitation abreview for Anti-SCAP antibody

     Average
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HuH7.5 (hepatocyte-derived carcinoma cell line))
    Total protein in input
    600 µg
    Immuno-precipitation step
    Protein A
    Specification
    HuH7.5 (hepatocyte-derived carcinoma cell line)
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 25 2017

    Western blot abreview for Anti-SCAP antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HuH7.5 Cell line ( hepatocyte-derived carcinoma ce)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    100 µg
    Specification
    HuH7.5 Cell line ( hepatocyte-derived carcinoma ce
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 17 2017

    Question

    Yes, I am interested in testing ab125186 in mouse or rat.

    Read More
    Answer

    DISCOUNT CODE: ***
    Expiration date: September 9, 2012

    This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab125186 using mouse or rat samplesand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Question

    Hi,
    The order confirmation number is 1031347.
    1) How much protein was loaded per lane of the gel?
    I didn't measure protein concentration. Cells were cultured in a 6-well plate, and were confluent when harvested. Each sample was collected by adding 200ul 1xSDS sample buffer to one well of the 6-well plate. 20ul of the indicated sample (1/10 of the lysate) was loaded per lane of the gel.
    2) What protease inhibitors were used, and was this a standard reduced and denatured Western blot?
    I used mammalian protease inhibitor cocktail. Yes it was a reduced and denatured western blot.
    3) What was used to block the membrane (i.e. milk or BSA)?
    For this experiment I used 5% BSA.
    4) What dilution of antibody was used?
    1:1000.
    Besides, I'd like to know more about the testing discount program. If the SCAP antibody (ab125186) works in human samples I would certainly test it on mouse or rat samples.
    Thanks,

    Read More
    Answer

    Thank you for your reply and for the additional information.

    I am sending a vial of ab86415 free of charge on the order ***. This antibody is in stock and should arrive on Monday. Please keep me updated about how this antibody works in your Western blots.

    Since Ab125186 has not been tested in mouse or rat samples, I can offer a discount off a future purchase if you buy the antibodynow, test it in one of these speciesand submit feedback to us in the form of an Abreview. The discount would be worth1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab125186 in mouse or rat. I will then send a discount code.

    2. Purchase ab125186 either by phone, fax, or online (www.abcam.com).

    3. Test it in mouse or rat.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab125186 turns out to be unsuitable for mouse or rat, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Question

    Hi,
    I am writing in regard to your anti-Insig2 antibody (ab86351), which hasn't been working for me at all. Attached is the western blot of one of my experiments, where I expressed FLAG-HA-tagged human Insig2 in HEK 293T cells, and blotted with anti-Insig2, anti-FLAG or anti-HA antibody. As you can see, anti-FLAG and anti-HA antibodies detected the tagged-INSIG2 pretty well, while no specific band showed up in the anti-Insig2 blot. I have also tried this antibody on rat and mouse liver lysates as well as HepG2 whole cell lysates and it could not detect the endogenous Insig2, either. I was wondering whether you could send me your other anti-Insig2 antibody (ab86415) as a replacement.
    Besides, I was wondering whether you could send me some samples of your anti-SCAP antibodies (ab125186 and ab91323). I would be happy to post my blots if the antibody works.
    Thanks,

    Read More
    Answer

    Thank you for contacting us and for letting us know about the trouble with ab86351. I am sorry to see that the results have been so poor.

    I would be happy to send a vial of ab86415 as a replacement. Do you have your original order or PO number, so that I can arrange for this replacement?

    For our records, I also have some additional questions about the protocol:

    1) How much protein was loaded per lane of the gel?
    2) What protease inhibitors were used, and was this a standard reduced and denatured Western blot?
    3) What was used to block the membrane (i.e. milk or BSA)?
    4) What dilution of antibody was used?

    We only have full size vials of all of our products, so unfortunately I am not able to send samples ofthe SCAP antibodies ab125186 and ab91323. We do guarantee them both to work in Western blot with human samples, so if you had any trouble with them we would issue replacements, credits, or refunds. If you would be using them in an untested species (like mouse or rat) we do have a testing discount program, and I would be happy to send more information about that program if it's applicable.

    I look forward to hearing from you and resolving this promptly. Please let me know if you have any questions or if there is anything else that we can do for you.

    Read More

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    For licensing inquiries, please contact partnerships@abcam.com