AB249081

重组Anti-SCAMP2/SC2抗体[EPR10185] - BSA and Azide free

Anti-SCAMP2/SC2 antibody [EPR10185] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal SCAMP2/SC2 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

查看别名

Secretory carrier-associated membrane protein 2, Secretory carrier membrane protein 2, SCAMP2

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAMP2/SC2 antibody [EPR10185] - BSA and Azide free (AB249081)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAMP2/SC2 antibody [EPR10185] - BSA and Azide free (AB249081)

This data was developed using ab154181, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling SCAMP2/SC2 with ab154181 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAMP2/SC2 antibody [EPR10185] - BSA and Azide free (AB249081)

This data was developed using ab154181, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human prostate hyperplasia tissue using ab154181 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAMP2/SC2 antibody [EPR10185] - BSA and Azide free (AB249081)

This data was developed using ab154181, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab154181 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAMP2/SC2 antibody [EPR10185] - BSA and Azide free (AB249081)

This data was developed using ab154181, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154181 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-SCAMP2/SC2 antibody [EPR10185] - BSA and Azide free (AB249081)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SCAMP2/SC2 antibody [EPR10185] - BSA and Azide free (AB249081)

This data was developed using ab154181, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized 293T cells with ab154181 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAMP2/SC2 antibody [EPR10185] - BSA and Azide free (AB249081)

This data was developed using ab154181, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human lung carcinoma tissue using ab154181 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-SCAMP2/SC2 antibody [EPR10185] - BSA and Azide free (AB249081)

This data was developed using ab154181, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SCAMP2/SC2 antibody [EPR10185] (<a href='/products/primary-antibodies/scamp2-sc2-antibody-epr10185-ab154181'>ab154181</a>) at 1/1000 dilution

Lane 1:

HepG2 lysates at 10 µg

Lane 2:

293T lysates at 10 µg

Lane 3:

Human tonsil lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 37 kDa,49 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-SCAMP2/SC2 antibody [EPR10185]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR10185

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

Flow Cyt (Intra), WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

ab249081 is the carrier-free version of ab154181.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SCAMP2 also known as SC2 is a small protein with a mass of approximately 33 kDa. It plays a role in secretory vesicle trafficking. SCAMP2 is widely expressed in various tissues including high levels in brain and endocrine tissues. This protein is embedded in the membrane of secretory vesicles which suggests an involvement in membrane trafficking processes. SCAMP2 possesses features such as coiled-coil domains important for intracellular trafficking functions.

Biological function summary

SCAMP2 is part of the intricate machinery regulating vesicle fusion and exocytosis. The protein participates in the transportation of vesicles between cell compartments ensuring the delivery of proteins and lipids to their target sites. SCAMP2 often functions as part of a larger complex involving other SCAMP family members enhancing vesicle recycling and secretion process efficiency. It regulates synaptic vesicle exocytosis which is essential for neurotransmitter release in the nervous system.

Pathways

The actions of SCAMP2 are integral in intracellular transport and exocytosis pathways. It interacts with SNARE proteins which are critical components of the membrane fusion machinery. SCAMP2 aids in coordinating the recruitment and assembly of SNARE complexes thereby facilitating efficient vesicle docking and fusion during neurotransmitter release. By engaging with proteins like syntaxin and VAMP2 SCAMP2 ensures proper communication between neurons playing an important role in synaptic transmission.

SCAMP2 is linked to neurological conditions such as epilepsy and neurodegenerative disorders. Alterations in SCAMP2 expression or function may affect synaptic transmission which can disrupt neural network stability and lead to disease states. It interacts with other neuronal proteins like synapsin and synaptophysin which may contribute to the development or progression of these conditions. Understanding SCAMP2's role can provide insight into therapeutic approaches for epilepsy and other related disorders.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Functions in post-Golgi recycling pathways. Acts as a recycling carrier to the cell surface.

See full target information Secretory carrier-associated membrane protein 2

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 60:2744-2760.e9 PubMed40460835

2025

ATG9A and ARFIP2 cooperate to control PI4P levels for lysosomal repair.

Applications

Unspecified application

Species

Unspecified reactive species

Stefano De Tito,Eugenia Almacellas,Daniel Dai Yu,Emily Millard,Wenxin Zhang,Cecilia de Heus,Christophe Queval,Javier H Hervás,Enrica Pellegrino,Ioanna Panagi,Ditte Fogde,Teresa L M Thurston,Judith Klumperman,Maximiliano Gutierrez,Sharon A Tooze

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-SCAMP2/SC2抗体[EPR10185] - BSA and Azide free