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Neuroscience Neurotransmission Secretory Vesicles Other

Anti-SCAMP1抗体(ab3430)

  • Datasheet
Submit a review Submit a question References (2)

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Western blot - Anti-SCAMP1 antibody (ab3430)
  • Western blot - Anti-SCAMP1 antibody (ab3430)
  • Western blot - Anti-SCAMP1 antibody (ab3430)

Key features and details

  • Rabbit polyclonal to SCAMP1
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-SCAMP1抗体
  • 描述

    兔多克隆抗体to SCAMP1
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Pig
  • 免疫原

    Synthetic peptide corresponding to Human SCAMP1 aa 1-17.
    Sequence:

    MSDFDSNPFADPDLNNP


    (Peptide available as ab4969)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • WB: U-87MG, Neuro-2a, SH-SY5Y, SK-N-AS, T98G, IMR-32, Rat brain tissue and Wild-type HeLa cell lysate.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • Other
    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3430于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 38 kDa).

By Western blot, this antibody detects an ~31 kDa protein representing SCAMP 1 rat brain homogenate.

说明
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 38 kDa).

By Western blot, this antibody detects an ~31 kDa protein representing SCAMP 1 rat brain homogenate.

靶标

  • 功能

    Functions in post-Golgi recycling pathways. Acts as a recycling carrier to the cell surface.
  • 组织特异性

    Widely expressed, with highest expression in brain.
  • 序列相似性

    Belongs to the SCAMP family.
  • 细胞定位

    Golgi apparatus > trans-Golgi network membrane. Recycling endosome membrane.
  • Target information above from: UniProt accession O15126 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 9522 Human
    • Entrez Gene: 107767 Mouse
    • Entrez Gene: 397477 Pig
    • Entrez Gene: 29521 Rat
    • Omim: 606911 Human
    • SwissProt: O15126 Human
    • SwissProt: Q8K021 Mouse
    • SwissProt: O77735 Pig
    • SwissProt: P56603 Rat
    • Unigene: 482587 Human
    • Unigene: 201455 Mouse
    • Unigene: 20374 Rat
    see all
  • 别名

    • SCAM1_HUMAN antibody
    • SCAMP 1 antibody
    • SCAMP 37 antibody
    • SCAMP antibody
    • SCAMP1 antibody
    • SCAMP37 antibody
    • Secretory carrier associated membrane protein 1 antibody
    • Secretory carrier membrane protein 1 antibody
    • Secretory carrier membrane protein antibody
    • Secretory carrier-associated membrane protein 1 antibody
    see all

图片

  • Western blot - Anti-SCAMP1 antibody (ab3430)
    Western blot - Anti-SCAMP1 antibody (ab3430)
    All lanes : Anti-SCAMP1 antibody (ab3430) at 1 µg/ml

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : SCAMP1 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 38 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?



    Lanes 1 - 2: Merged signal (red and green). Green - ab3430 observed at 36 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

    ab3430 was shown to react with SCAMP1 in wild-type HeLa cells in western blot. The bands observed in SCAMP1 knockout cell line ab265567 (SCAMP1 knockout cell lysate ab258184) below 36 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. HeLa wild-type and SCAMP1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab3430 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at 1 µg/ml and a 1/20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-SCAMP1 antibody (ab3430)
    Western blot - Anti-SCAMP1 antibody (ab3430)
    All lanes : Anti-SCAMP1 antibody (ab3430) at 2 µg/ml

    Lane 1 : U-87MG (Human primary glioblastoma cell line) lysate
    Lane 2 : Neuro-2a (Mouse brain) lysate
    Lane 3 : SH-SY5Y (Human derived cell line) lysate
    Lane 4 : SK-N-AS (Human neuroblastoma cell line) lysate
    Lane 5 : T98G (Glioblastoma cell line) lysate
    Lane 6 : IMR-32 (Human neuroblastoma cell line) lysate
    Lane 7 : Rat brain tissue

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L), HRP conjugate at 1/4000 dilution

    Predicted band size: 38 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% Milk.

  • Western blot - Anti-SCAMP1 antibody (ab3430)
    Western blot - Anti-SCAMP1 antibody (ab3430)

    Western blot detection of SCAMP1 on rat brain extract using ab3430.

     

实验方案

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

数据表及文件

  • Datasheet download

    Download

文献 (2)

发表研究结果有使用 ab3430?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab3430 被引用在 2 文献中.

  • Gerlini R  et al. Double knock-out of Hmga1 and Hipk2 genes causes perinatal death associated to respiratory distress and thyroid abnormalities in mice. Cell Death Dis 10:747 (2019). PubMed: 31582725
  • Jain P  et al. Identification of human T cell leukemia virus type 1 tax amino acid signals and cellular factors involved in secretion of the viral oncoprotein. J Biol Chem 282:34581-93 (2007). WB, Dot blot ; Hamster . PubMed: 17897946

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