Anti-SCAMP1抗体

• WB

Supplier Data

Western blot - Anti-SCAMP1 antibody (AB3430)

Lanes 1 - 2 : Merged signal (red and green). Green - ab3430 observed at 36 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa. ab3430 was shown to react with SCAMP1 in wild-type HeLa cells in western blot. The bands observed in SCAMP1 knockout cell line ab265567 (SCAMP1 knockout cell lysate ab258184) below 36 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. HeLa wild-type and SCAMP1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab3430 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at 1 µg/ml and a 1/20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-SCAMP1 antibody (ab3430) at 1 µg/mL

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

SCAMP1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human SCAMP1 knockout HeLa cell line (<a href='/products/cell-lines/human-scamp1-knockout-hela-cell-line-ab265567'>ab265567</a>)

Secondary

Lanes 1 - 2:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution

Lanes 1 - 2:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 38 kDa

Observed band size: 36 kDa

false

• WB

Unknown

Western blot - Anti-SCAMP1 antibody (AB3430)

Western blot detection of SCAMP1 on rat brain extract using ab3430.

All lanes:

Western blot - Anti-SCAMP1 antibody (ab3430)

Predicted band size: 38 kDa

false

• WB

Supplier Data

Western blot - Anti-SCAMP1 antibody (AB3430)

Blocking buffer : 5% Milk.

All lanes:

Western blot - Anti-SCAMP1 antibody (ab3430) at 2 µg/mL

Lane 1:

U-87MG (Human primary glioblastoma cell line) lysate at 30 µg

Lane 2:

Neuro-2a (Mouse brain) lysate at 30 µg

Lane 3:

SH-SY5Y (Human derived cell line) lysate at 30 µg

Lane 4:

SK-N-AS (Human neuroblastoma cell line) lysate at 30 µg

Lane 5:

T98G (Glioblastoma cell line) lysate at 30 µg

Lane 6:

IMR-32 (Human neuroblastoma cell line) lysate at 30 µg

Lane 7:

Rat brain tissue at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L), HRP conjugate at 1/4000 dilution

Predicted band size: 38 kDa

Observed band size: 36 kDa

false