AB181106

重组Anti-Sbf1抗体[EPR12567]

Name: Sbf1抗体[EPR12567] (ab181106)| Abcam中文官网
Brand: Abcam Limited
SKU: AB181106
Price: 2466 CNY
Availability: InStock
Rating: 5 (1 reviews)

Anti-Sbf1 antibody [EPR12567]

RabMAb
Recombinant
了解详情

(1 Review)

(2 Publications)

Rabbit Recombinant Monoclonal Sbf1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications.

查看别名

MTMR5, SBF1, Myotubularin-related protein 5, Inactive phosphatidylinositol 3-phosphatase 5, SET-binding factor 1, Sbf1

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sbf1 antibody [EPR12567] (AB181106)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sbf1 antibody [EPR12567] (AB181106)

Immunohistochemical analysis of paraffin-embedded Human muscle tissue labeling Sbf1 with unpurified ab181106 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sbf1 antibody [EPR12567] (AB181106)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Sbf1 with unpurified ab181106 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sbf1 antibody [EPR12567] (AB181106)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Sbf1 with purified ab181106 at 1/2000 dilution (0.54 μg/ml). Heat mediated antigen retrieval using Bond Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sbf1 antibody [EPR12567] (AB181106)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling Sbf1 with purified ab181106 at 1/2000 dilution (0.54 μg/ml). Heat mediated antigen retrieval using Bond Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Lab

Western blot - Anti-Sbf1 antibody [EPR12567] (AB181106)

We are unsure how to define the extra bands.

All lanes:

Western blot - Anti-Sbf1 antibody [EPR12567] (ab181106) at 1/1000 dilution

Lane 1:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates at 15 µg

Lane 2:

Human brain lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 208 kDa

Observed band size: 208 kDa

false

Lab

Western blot - Anti-Sbf1 antibody [EPR12567] (AB181106)

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/1000000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative controls : mouse ovary, small intestine (PMID : 11994405).

All lanes:

Western blot - Anti-Sbf1 antibody [EPR12567] (ab181106) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 20 µg

Lane 2:

Mouse ovary tissue lysate at 20 µg

Lane 3:

Mouse small intestine tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 208 kDa,37 kDa

false

Exposure time: 20s

Lab

Western blot - Anti-Sbf1 antibody [EPR12567] (AB181106)

We are unsure how to define the extra bands.

All lanes:

Western blot - Anti-Sbf1 antibody [EPR12567] (ab181106) at 1/1000 dilution

Lane 1:

Mouse cerebellum lysates at 15 µg

Lane 2:

Mouse testis lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 208 kDa

Observed band size: 208 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-Sbf1 antibody [EPR12567] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12567

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "For unpurified use at 1/250 - 1/500. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "For unpurified use at 1/250 - 1/500. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Sbf1 also known as SET-binding factor 1 mechanistically acts as a pseudophosphatase due to having a phosphatase sequence motif but lacking phosphatase activity. Sbf1 with a molecular weight of approximately 206 kDa is expressed in various tissues including the brain and the immune system. It belongs to a protein family associated with cellular regulation and its pseudophosphatase domain is significant for its interactions with other proteins.

Biological function summary

Sbf1 contributes to cellular regulation and signaling pathways. It is known for its involvement in regulating cell growth and migration. Sbf1 does not act alone often forming complexes with other proteins that modulate its function. Researchers have noted its role in cellular structures involved in maintaining the cytoskeleton and intercellular connections which further implicates it in essential cellular processes.

Pathways

Sbf1 interacts within the mTOR signaling pathway a critical pathway for regulating cell growth and metabolism. It also plays a role in the PI3K/AKT pathway important for controlling various cellular processes including proliferation and survival. Through these pathways Sbf1 interacts with proteins such as mTOR and AKT contributing to maintaining cellular functions related to growth signals and nutrient sensing.

Sbf1 connects to cancer and neurological disorders. Mutations or altered expressions in Sbf1 have been observed in certain types of cancer including breast cancer where it can affect cellular proliferation and survival. Additionally its association with proteins involved in neurological processes suggests a potential link to neurodegenerative disorders. Proteins such as mTOR in cancer and AKT in neurological disorders provide direct connections to Sbf1 highlighting its role in pathways related to these diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Acts as an adapter for the phosphatase MTMR2 to regulate MTMR2 catalytic activity and subcellular location (PubMed : 12668758). Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form (PubMed : 20937701). May function as a guanine nucleotide exchange factor (GEF) activating RAB28 (PubMed : 20937701). Acts as a suppressor of autophagy in neurons (PubMed : 35580604). Together with its binding partner, the phosphatase MTMR2, plays a role in dephosphorylation of phosphoinositides critical for autophagy initiation and autophagosome maturation (PubMed : 35580604). Plays a role in positively regulating late-stage radial sorting of large caliber axons, a process leading to myelination by Schwann cells, possibly via regulating endosomal trafficking (By similarity). Inhibits myoblast differentiation in vitro and induces oncogenic transformation in fibroblasts (PubMed : 9537414).

See full target information SBF1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Current biology : CB 32:2581-2595.e6 PubMed35580604

2022

Myotubularin-related phosphatase 5 is a critical determinant of autophagy in neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Jason P Chua,Karan Bedi,Michelle T Paulsen,Mats Ljungman,Elizabeth M H Tank,Erin S Kim,Jonathon P McBride,Jennifer M Colón-Mercado,Michael E Ward,Lois S Weisman,Sami J Barmada

Reproduction (Cambridge, England) 152:215-23 PubMed27335132

2016

Splicing mutation in Sbf1 causes nonsyndromic male infertility in the rat.

Applications

Unspecified application

Species

Unspecified reactive species

František Liška,Blanka Chylíková,Michaela Janků,Ondřej Šeda,Zdeňka Vernerová,Michal Pravenec,Vladimír Křen

View all publications

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重组Anti-Sbf1抗体[EPR12567]