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重组RabMAb

重组Anti-Sbf1抗体[EPR12566] (ab177146)

Submit a review Submit a question References (1)
Flow Cytometry (Intracellular) - Anti-Sbf1 antibody [EPR12566] (ab177146)
  • Western blot - Anti-Sbf1 antibody [EPR12566] (ab177146)
  • Anti-Sbf1 antibody [EPR12566] (ab177146)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12566] to Sbf1
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Sbf1抗体[EPR12566]
    参阅全部 Sbf1 一抗

  • 描述

    兔单克隆抗体[EPR12566] to Sbf1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WBmore details
    不适用于: ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human Sbf1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: O95248

  • 阳性对照

    • Raji, Ramos, Daudi and Human fetal brain lysates; permeabilized Ramos cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR12566

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab177146于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 208 kDa.
说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 208 kDa.
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.

靶标

  • 相关性

    Probable pseudophosphatase. Lacks several amino acids in the catalytic pocket which renders it catalytically inactive as a phosphatase. The pocket is however sufficiently preserved to bind phosphorylated substrates, and maybe protect them from phosphatases. Inhibits myoblast differentiation in vitro and induces oncogenic transformation in fibroblasts. May function as a guanine nucleotide exchange factor (GEF) activating RAB28. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form.

  • 细胞定位

    Nuclear

  • 数据库链接

    • 别名

      • C22:RP4-579N16.2 antibody
      • CMT4B3 antibody
      • DENN/MADD domain containing 7A antibody
      • DENND7A antibody
      • MTMR5 antibody
      • Myotubularin related 5 antibody
      • Myotubularin-related protein 5 antibody
      • SET-binding factor 1 antibody
      see all

    图片

    • Flow Cytometry (Intracellular) - Anti-Sbf1 antibody [EPR12566] (ab177146)
      Flow Cytometry (Intracellular) - Anti-Sbf1 antibody [EPR12566] (ab177146)

      Intracellular flow cytometric analysis of permeabilized Ramoscells labeling Sbf1with ab177146 at 1/10 dilution (red), or a rabbit IgG (negative) (green). 

       

       

    • Western blot - Anti-Sbf1 antibody [EPR12566] (ab177146)
      Western blot - Anti-Sbf1 antibody [EPR12566] (ab177146)
      All lanes : Anti-Sbf1 antibody [EPR12566] (ab177146) at 1/1000 dilution

      Lane 1 : Raji lysate
      Lane 2 : Ramos lysate
      Lane 3 : Daudi lysate
      Lane 4 : Human fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 208 kDa

    • Anti-Sbf1 antibody [EPR12566] (ab177146)
      Anti-Sbf1 antibody [EPR12566] (ab177146)

    文献 (1)

    发表研究结果有使用 ab177146？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab177146 被引用在 1 文献中.

    • Liška F  et al. Splicing mutation in Sbf1 causes nonsyndromic male infertility in the rat. Reproduction 152:215-23 (2016). PubMed: 27335132

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