重组Anti-SBDS抗体[EPR7820] (ab128946)
![Western blot - Anti-SBDS antibody [EPR7820] (ab128946)](https://www.abcam.cn/ps/products/128/ab128946/Images/ab128946-142828-anti-sbds-antibody-epr7820-western-blot.jpg "Western blot - Anti-SBDS antibody [EPR7820] (ab128946)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7820] to SBDS
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-SBDS抗体[EPR7820]
参阅全部 SBDS 一抗 -
描述
兔单克隆抗体[EPR7820] to SBDS -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt or IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human SBDS aa 200-300 (C terminal). The exact sequence is proprietary.
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阳性对照
- 293T, HepG2, Caco 2 cell lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 1.88 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7820 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128946于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 29 kDa.
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| 说明 |
|---|
|
WB
1/10000 - 1/50000. Predicted molecular weight: 29 kDa. |
应用说明
Is unsuitable for Flow Cyt or IHC-P.
靶标
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功能
Required for the assembly of mature ribosomes and ribosome biogenesis. Together with EFTUD1, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Required for normal levels of protein synthesis. May play a role in cellular stress resistance. May play a role in cellular response to DNA damage. May play a role in cell proliferation. -
组织特异性
Widely expressed. -
疾病相关
Defects in SBDS are the cause of Shwachman-Diamond syndrome (SDS) [MIM:260400]. SDS is an autosomal recessive disorder characterized by pancreatic exocrine insufficiency, hematologic dysfunction, and skeletal abnormalities. -
序列相似性
Belongs to the SDO1/SBDS family. -
细胞定位
Cytoplasm. Nucleus > nucleolus. Nucleus > nucleoplasm. Cytoplasm > cytoskeleton > spindle. Primarily detected in the cytoplasm, and at low levels in nucleus and nucleolus (PubMed:19602484 and PubMed:17475909). Detected in the nucleolus during G1 and G2 phase of the cell cycle, and diffusely distributed in the nucleus during S phase. Detected at the mitotic spindle. Colocalizes with the microtubule organizing center during interphase. - Information by UniProt
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数据库链接
- Entrez Gene: 51119 Human
- Entrez Gene: 66711 Mouse
- Entrez Gene: 288615 Rat
- Omim: 607444 Human
- SwissProt: Q9Y3A5 Human
- SwissProt: P70122 Mouse
- SwissProt: Q5RK30 Rat
- Unigene: 110445 Human
see all -
别名
- 4733401P19Rik antibody
- AI836084 antibody
- CGI 97 antibody
see all
图片
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Western blot - Anti-SBDS antibody [EPR7820] (ab128946)All lanes : Anti-SBDS antibody [EPR7820] (ab128946) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Caco 2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 29 kDa -
![OI-RD Scanning - Anti-SBDS antibody [EPR7820] (ab128946)](https://www.abcam.cn/ps/products/128/ab128946/Images/ab128946-142827-anti-sbds-antibody-epr7820.jpg)
OI-RD Scanning - Anti-SBDS antibody [EPR7820] (ab128946) -
![Anti-SBDS antibody [EPR7820] (ab128946)](https://www.abcam.cn/ps/products/128/ab128946/Images/ab128946-3-benefits-of-recombinant-antibodies.png)
Anti-SBDS antibody [EPR7820] (ab128946)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab128946 被引用在 4 文献中.
- Bezzerri V et al. Novel Translational Read-through-Inducing Drugs as a Therapeutic Option for Shwachman-Diamond Syndrome. Biomedicines 10:N/A (2022). PubMed: 35453634
- Pantazi A et al. Inhibition of the 60S ribosome biogenesis GTPase LSG1 causes endoplasmic reticular disruption and cellular senescence. Aging Cell 18:e12981 (2019). PubMed: 31148378
- Qiao H et al. Enolase1 overexpression regulates the growth of gastric cancer cells and predicts poor survival. J Cell Biochem 120:18714-18723 (2019). PubMed: 31218757
- Carvalho CM et al. Structural variation and missense mutation in SBDS associated with Shwachman-Diamond syndrome. BMC Med Genet 15:64 (2014). WB . PubMed: 24898207