AB85993

Anti-SASS6抗体

Name: SASS6抗体 (ab85993)| Abcam中文官网
Brand: Abcam Limited
SKU: AB85993
Price: 4606 CNY
Availability: InStock

Anti-SASS6 antibody

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(1 Publication)

Rabbit Polyclonal SASS6 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SASS6 aa 600 to C-terminus.

查看别名

SAS6, SASS6, Spindle assembly abnormal protein 6 homolog, HsSAS-6, Spindle assembly defective protein 6

2 Images

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Immunoprecipitation - Anti-SASS6 antibody (AB85993)

Detection of Human SASS6 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85993 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-SASS6 antibody (ab85993)

Predicted band size: 74 kDa

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Western blot - Anti-SASS6 antibody (AB85993)

All lanes:

Western blot - Anti-SASS6 antibody (ab85993) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 74 kDa

Observed band size: 74 kDa

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Exposure time: 3min

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IP, WB

applications

免疫原

Synthetic Peptide within Human SASS6 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6UVJ0

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SASS6 also known as spindle assembly 6 homolog is a protein that plays an important role in centriole biogenesis. It has a molecular mass of approximately 82 kDa. SASS6 expresses not only in dividing cells but also in tissues where active cell division takes place including the brain testis and other proliferative tissues. Its distribution indicates its involvement in cellular processes that require precise organelle replication and segregation.

Biological function summary

SASS6 integrates into a complex essential for centriole duplication which is fundamental for accurate mitotic spindle formation. It forms a structural core in the assembling centrioles ensuring their cylindrical shape. The protein helps in organizing microtubule triplets around a central hub which is vital for proper cell division and maintenance of genomic stability.

Pathways

The protein contributes significantly to the cell cycle regulation and centrosome cohesion. It participates in the centriole duplication pathway which interacts with other proteins like STIL and CEP135. Through this interaction SASS6 ensures efficient progression and regulation within the centrosome cycle which is critical for cell cycle checkpoints and prevention of aneuploidy.

Mutations or dysregulation of SASS6 are linked to microcephaly and other developmental anomalies. Such conditions often result from improper centriole formation impacting neurogenesis and brain size. The involvement of SASS6 also cross-references with proteins such as CEP63 in these disorders highlighting a shared pathway impacting centrosome-related abnormalities.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Central scaffolding component of the centrioles ensuring their 9-fold symmetry (By similarity). Required for centrosome biogenesis and duplication : required both for mother-centriole-dependent centriole duplication and deuterosome-dependent centriole amplification in multiciliated cells (PubMed : 15665853, PubMed : 16244668, PubMed : 17681131). Not required for centriole formation in embryonic stem cells but necessary to maintain centriole architecture (By similarity). Required for the recruitment of STIL to the procentriole and for STIL-mediated centriole amplification (PubMed : 22020124). Overexpression results in excess foci-bearing centriolar markers (PubMed : 15665853).

See full target information SASS6

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 12:760999 PubMed34804049

2021

Inhibitor of Differentiation-2 Protein Ameliorates DSS-Induced Ulcerative Colitis by Inhibiting NF-κB Activation in Neutrophils.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Ren,Dong Yan,Yichun Wang,Jiaojiao Zhang,Min Li,Wancheng Xiong,Xueqian Jing,Puze Li,Weidong Zhao,Xiwen Xiong,Minna Wu,Genshen Zhong

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Anti-SASS6抗体

Anti-SASS6 antibody

Immunoprecipitation - Anti-SASS6 antibody (AB85993)

Western blot - Anti-SASS6 antibody (AB85993)