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Anti-SARS spike glycoprotein抗体[1A9] (ab273433)

Reviews (1) Submit a question References (21)
Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
  • Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
  • Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
  • Immunocytochemistry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
  • Immunocytochemistry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
  • Immunocytochemistry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
  • Flow Cytometry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
  • Sandwich ELISA - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
  • Immunoprecipitation - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

Key features and details

  • Mouse monoclonal [1A9] to SARS spike glycoprotein
  • Suitable for: ICC, WB, Flow Cyt, IP, Sandwich ELISA
  • Isotype: IgG1

概述

  • 产品名称

    Anti-SARS spike glycoprotein抗体[1A9]
    参阅全部 SARS spike glycoprotein 一抗

  • 描述

    小鼠单克隆抗体[1A9] to SARS spike glycoprotein

  • 宿主

    Mouse

  • 经测试应用

    适用于: ICC, WB, Flow Cyt, IP, Sandwich ELISAmore details

  • 种属反应性

    与反应: SARS-CoV, SARS-CoV-2

  • 免疫原

    Recombinant fragment within Human coronavirus SARS spike glycoprotein aa 1000-1200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • WB: Infected Caco-2 cells; SARS-CoV-2 transfected HEK-293T cell lysate; SARS-Cov1 spike protein transfected Expi cell lysate, SARS-Cov1 3xFlag spike protein transfected Expi cell lysate, SARS-Cov2 spike protein transfected Expi cell lysate and SARS-Cov2 3xFlag spike protein transfected Expi cell lysate. ICC: SARS-CoV-2 transfected BHK-21, Vero E6, COS-7 cells. IP: SARS-CoV-2 transfected HEK-293T cells. Flow Cyt: Infected HEK-293T cells.

  • 常规说明

    Applications overview

    Tick: Tested and Guaranteed to work      X: Will not work      —: No data

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab273433于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC (1)
1/100 - 1/2000.
WB
1/500 - 1/3000. Predicted molecular weight: 139 kDa.
Flow Cyt
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Sandwich ELISA
Use a concentration of 5 µg/ml.
说明
ICC
1/100 - 1/2000.
WB
1/500 - 1/3000. Predicted molecular weight: 139 kDa.
Flow Cyt
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Sandwich ELISA
Use a concentration of 5 µg/ml.

靶标

  • 相关性

    A novel coronavirus has been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive stranded RNA approximately 27 to 31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139 kDa protein and the major surface antigen of the virus, to the angiotensin converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2.

  • 别名

    • E2 antibody
    • E2 glycoprotein antibody
    • Human coronavirus spike glycoprotein antibody
    • S antibody
    • S glycoprotein antibody
    • Spike glycoprotein antibody
    see all

图片

  • Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Lane 1 : Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Lanes 2-3 : Anti-SARS spike glycoprotein antibody [1A9] (ab273433) at 1/1000 dilution

    Lane 1 : Mock
    Lane 2 : SARS-CoV infected Caco-2 whole cell extract
    Lane 3 : SARS-CoV-2 infected Caco-2 whole cell extract

    Predicted band size: 139 kDa

  • Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    All lanes : Anti-SARS spike glycoprotein antibody [1A9] (ab273433) at 1/1000 dilution

    Lane 1 : Non-transfected HEK-293T whole cell extract
    Lane 2 : Transfected HEK-293T whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-mouse IgG antibody

    Predicted band size: 139 kDa

  • Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    All lanes : Anti-SARS spike glycoprotein antibody [1A9] (ab273433) at 1 µg/ml

    Lane 1 : Mock transfected human Expi cell lysate
    Lane 2 : PDL-1 Flag transfected human Expi cell lysate
    Lane 3 : SARS-Cov1 spike protein transfected human Expi cell lysate
    Lane 4 : SARS-Cov1 3xFlag spike protein transfected human Expi cell lysate
    Lane 5 : SARS-Cov2 spike protein transfected human Expi cell lysate
    Lane 6 : SARS-Cov2 3xFlag spike protein transfected human Expi cell lysate
    Lane 7 : MERS Spike protein transfected human Expi cell lysate
    Lane 8 : MERS 3xFlag Spike protein transfected human Expi cell lysate

    Lysates/proteins at 10 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 139 kDa
    Observed band size: 200 kDa why is the actual band size different from the predicted?



    Lanes 1 - 8: Merged signal (red and green). Green - ab273433 observed at 200 kDa. Red - loading control.

    ab273433 was shown to react with SARS spike glycoprotein in western blot. Membranes were blocked in 3% milk before incubation with ab273433 overnight at 4°C at 1 ug/ml. Blots were incubated with Donkey anti-Mouse IgG H&L(IRDye® 800CW) preabsorbed (ab216774) antibody at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Immunocytochemistry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

    4% paraformaldehyde-fixed BHK-21 cells stained for SARS-CoV-2 (COVID-19) spike (green) using ab273433 at 1/2000 dilution in ICC.

  • Immunocytochemistry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Immunocytochemistry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

    Methanol-fixed Vero E6 cells infected with SARS-CoV-2 (MOI of 1) stained for SARS-CoV-2 (green) using ab273433 at 1/2000 dilution in ICC. Counterstained with DAPI (Blue).

  • Immunocytochemistry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Immunocytochemistry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

    Mock and SARS-CoV-2-transfected COS-7 cells stained for SARS-CoV-2 (COVID-19) spike (green) using ab273433 at 1/2000 dilution in ICC. Nuclei were counterstained with DAPI (Blue).

  • Flow Cytometry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Flow Cytometry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

    Flow cytometric analysis of HEK-293T cells infected with recombinant vaccinia virus carrying the spike gene, using ab273433.

  • Sandwich ELISA - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Sandwich ELISA - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

    Sandwich ELISA detection of SARS spike glycoprotein using ab273433 as the capture antibody at 5 µg/mL. An alternative antibody was used as the detection antibody at 1 µg/mL followed by a Rabbit IgG antibody (HRP) at 1/10000 to detect the primary antibody.

  • Immunoprecipitation - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Immunoprecipitation - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

    SARS-CoV-2 Spike was immunoprecipitated from 2μg of HEK-293T whole cell lysate with ab273433. Western blot was performed from the immunoprecipitate using ab273433. EasyBlot HRP-conjugated anti mouse IgG antibody.


    Lane 1: HEK-293T lysate 2 μg (Input).
    Lane 2: Control IgG IP in HEK-293T lysate.
    Lane 3: ab273433 IP in HEK-293T lysate.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (21)

发表研究结果有使用 ab273433？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab273433 被引用在 21 文献中.

  • Nchioua R  et al. Reduced replication but increased interferon resistance of SARS-CoV-2 Omicron BA.1. Life Sci Alliance 6:N/A (2023). PubMed: 36977594
  • Zuniga-Hertz JP  et al. Meditation-induced bloodborne factors as an adjuvant treatment to COVID-19 disease. Brain Behav Immun Health 32:100675 (2023). PubMed: 37600600
  • Cacciottolo M  et al. Nanograms of SARS-CoV-2 spike protein delivered by exosomes induce potent neutralization of both delta and omicron variants. PLoS One 18:e0290046 (2023). PubMed: 37607200
  • Ouyang MJ  et al. Oral Immunization with rVSV Bivalent Vaccine Elicits Protective Immune Responses, Including ADCC, against Both SARS-CoV-2 and Influenza A Viruses. Vaccines (Basel) 11:N/A (2023). PubMed: 37766083
  • Guo C  et al. The D614G mutation redirects SARS-CoV-2 spike to lysosomes and suppresses deleterious traits of the furin cleavage site insertion mutation. Sci Adv 8:eade5085 (2022). PubMed: 36563151
View all Publications for this product

客户评价及客户问答

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Immunocytochemistry abreview for Anti-SARS spike glycoprotein antibody [1A9]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry
Sample
SARS-CoV-2 Cultured Cells (Supernatant of Spodoptera frugiperda (Sf9) culture)
Permeabilization
No
Specification
Supernatant of Spodoptera frugiperda (Sf9) culture
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Oct 04 2023

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