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重组

重组Anti-SARS-CoV-2 Spike RBD抗体[HL1014] - BSA and Azide free (ab281305)

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Indirect ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)
  • Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)
  • Immunocytochemistry - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)
  • Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)
  • Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)
  • Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)
  • Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)
  • Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)
  • Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [HL1014] to SARS-CoV-2 Spike RBD - BSA and Azide free
  • Suitable for: ICC, Sandwich ELISA, Indirect ELISA

概述

  • 产品名称

    Anti-SARS-CoV-2 Spike RBD抗体[HL1014] - BSA and Azide free
    参阅全部 SARS-CoV-2 Spike RBD 一抗

  • 描述

    兔单克隆抗体[HL1014] to SARS-CoV-2 Spike RBD - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC, Sandwich ELISA, Indirect ELISAmore details

  • 种属反应性

    与反应: SARS-CoV-2

  • 免疫原

    Recombinant fragment within Human coronavirus SARS-CoV-2 Spike RBD. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Strain Wuhan-Hu-1

  • 阳性对照

    • ICC: Mock and transfected HEK-293T cells. ELISA: Recombinant full-length SARS-CoV-2 spike (trimer) protein, recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, SARS-CoV-2 (COVID-19) Spike transfected HEK-293T cell extracts.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab281305于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC
1/100 - 1/2500.
Sandwich ELISA
Use at an assay dependent concentration.
Indirect ELISA
Use at an assay dependent concentration.
说明
ICC
1/100 - 1/2500.
Sandwich ELISA
Use at an assay dependent concentration.
Indirect ELISA
Use at an assay dependent concentration.

图片

  • Indirect ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)
    Indirect ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)

    Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 spike (trimer) protein (50 ng). Coated protein probed with ab281305 (160-1.91*10-5 nM). HRP-Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody. EC50 : 28.77 pM.

  • Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)
    Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)

    Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein using ab281305 as capture antibody at concentration of 5 μg/mL and a HRP-conjugated ab281304 as detection antibody at concentration of 1 μg/mL.

  • Immunocytochemistry - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)
    Immunocytochemistry - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)

    Mock and transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) Spike RBD stained (green) by ab281305 at 1/2500 dilution immunocytochemical analysis. Fluoroshield with DAPI (blue).

  • Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)
    Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)

    Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using ab281305 as capture antibody at concentration of 5 μg/mL and HRP-conjugated ab281304 as detection antibody at concentration of 1 μg/mL.

  • Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)
    Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)

    Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (HexaPro) ECD, His tag using ab281305 as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody as detection antibody at concentration of 1 μg/mL.

  • Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)
    Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)

    Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) using ab281305 as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody as detection antibody at concentration of 1 μg/mL.

  • Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)
    Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)

    Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) using ab281305 as capture antibody at 5 µg/mL and HRP-conjugated ab281304 as detection antibody at concentration of 1 µg/mL.

  • Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)
    Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)

    Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein using ab281305 as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody as detection antibody at concentration of 1 μg/mL.

  • Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)
    Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)

    Sandwich ELISA detection of non-transfected and SARS-CoV-2 (COVID-19) Spike, MERS-CoV Spike, or SARS-CoV Spike transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts using ab281305 as capture antibody at concentration of 5 μg/mL and HRP-conjugated ab281304 as detection antibody at concentration of 1 μg/mL.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

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