重组Anti-SARS-CoV-2 Spike RBD抗体[HL1003] - BSA and Azide free (ab281303)

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 spike (trimer) protein (50 ng). Coated protein probed with ab281303 (20-4.8*10^-6 nM). HRP-Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody.
EC50: 7.49 pM

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using a SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody as capture antibody at concentration of 5 μg/mL andab281303 as detection antibody at concentration of 1 μg/mL. HRP-Rabbit IgG antibody was diluted at 1/10000 dilution and used to detect the primary antibody.

Mock and transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) Spike RBD stained (green) by ab281303 at 1/700 dilution in immunocytochemical analysis. Fluoroshield with DAPI (blue).

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein using ab281305 as capture antibody at concentration of 5 μg/mL and HRP-conjugated ab281303 as detection antibody at concentration of 1 μg/mL.

Please notice that ab281303 needs to be conjugated to HRP to function as the detection antibody.

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using ab281305 as capture antibody at concentration of 5 μg/mL and HRP-conjugated ab281303 as detection antibody at concentration of 1 μg/mL.

Please notice that ab281303 needs to be conjugated to HRP to function as the detection antibody.

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (coated at 2 µg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (3000 ng/mL). ACE2 binding was inhibited by increasing concentrations of ab281303 (14-10000 ng/mL). Bound ACE2 protein was detected by HRP-Goat Anti-Mouse IgG antibody at 1/10000 dilution.

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein (4000-62.5 ng/mL). Coated protein probed with ab281303 at 1 µg/mL. HRP-Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody.

ab281303 effectively neutralizes SARS-CoV-2 pseudovirus entry into human cells. Control or tested antibodies were mixed with GFP-expressing SARS-CoV-2 (wild-type) pseudovirus. Each mixture was then added to HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate overexpressing human ACE2. After 48 hours, the infections were observed by detecting GFP using fluorescence microscopy.

SARS-CoV-2 (COVID-19) Spike RBD antibody (HL1003) effectively neutralizes SARS-CoV-2 pseudovirus entry into human cells. Control or tested antibodies were mixed with GFP-expressing SARS-CoV-2 (Furin modified) pseudovirus. Each mixture was then added to HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate overexpressing human ACE2. After 48 hours, the infections were observed by detecting GFP using fluorescence microscopy.