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重组

重组Anti-SARS-CoV-2 Spike Glycoprotein S2抗体[HL237] - BSA and Azide free (ab281312)

Reviews (1) Submit a question References (2)
Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)
  • Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)
  • Sandwich ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)
  • Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [HL237] to SARS-CoV-2 Spike Glycoprotein S2 - BSA and Azide free
  • Suitable for: WB, IHC-P, Sandwich ELISA, ICC/IF

概述

  • 产品名称

    Anti-SARS-CoV-2 Spike Glycoprotein S2抗体[HL237] - BSA and Azide free
    参阅全部 SARS-CoV-2 Spike Glycoprotein S2 一抗

  • 描述

    兔单克隆抗体[HL237] to SARS-CoV-2 Spike Glycoprotein S2 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-P, Sandwich ELISA, ICC/IFmore details

  • 种属反应性

    与反应: SARS-CoV-2

  • 免疫原

    Synthetic peptide within Human coronavirus SARS-CoV-2 Spike Glycoprotein S2. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Carrier-protein conjugated synthetic peptide SARS-CoV-2 (strain Wuhan-Hu-1)

  • 阳性对照

    • IHC-P: SARS-CoV-2 (COVID-19) Spike transfected 293T cell ICC/IF: SARS-CoV-2 (COVID-19) Spike transfected 293T cells. WB: SARS-CoV-2 (COVID-19) Spike transfected 293T cells. ELISA: Recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab281312于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000.
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA
Use a concentration of 1 µg/ml.
ICC/IF
1/100 - 1/1000.
说明
WB
1/1000 - 1/10000.
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA
Use a concentration of 1 µg/ml.
ICC/IF
1/100 - 1/1000.

图片

  • Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)
    Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)
    All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312) at 1/5000 dilution

    Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : SARS-CoV Spike transfected 293T whole cell extract
    Lane 3 : SARS-CoV-2 (COVID-19) Spike transfected 293T whole cell extract
    Lane 4 : MERS Spike transfected 293T whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody


    5% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)

    Mock and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block. SARS-CoV-2 (COVID-19) Spike S2 stained (green) by ab281312 at 1/1000 dilution in immunohistochemical analysis. Fluoroshield with DAPI (blue).

    Antigen Retrieval was achieved with Citrate buffer at pH 6.0 for 15 minutes.

  • Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)
    Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)

    Mock and SARS-CoV-2 (COVID-19) Spike transfected 293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) Spike S2 stained (green) by ab281312 at 1/1000 dilution immunocytochemical analysis. Fluoroshield with DAPI (blue).

  • Sandwich ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)
    Sandwich ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)

    Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag protein using a SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody as capture antibody at 5 µg/mL and ab281312 as detection antibody at 1 µg/mL. HRP-conjugated Rabbit IgG antibody at 1/10000 dilution and used to detect the primary antibody.

  • Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)
    Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)
    All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312) at 1/5000 dilution

    Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : SARS-CoV-2 (COVID-19) Spike transfected 293T whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody


    5% SDS-PAGE

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (2)

发表研究结果有使用 ab281312？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab281312 被引用在 2 文献中.

  • Boix-Besora A  et al. Gag Virus-like Particles Functionalized with SARS-CoV-2 Variants: Generation, Characterization and Recognition by COVID-19 Convalescent Patients' Sera. Vaccines (Basel) 11:N/A (2023). PubMed: 38005972
  • Boix-Besora A  et al. Optimization, Production, Purification and Characterization of HIV-1 GAG-Based Virus-like Particles Functionalized with SARS-CoV-2. Vaccines (Basel) 10:N/A (2022). PubMed: 35214708

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Immunocytochemistry abreview for Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free

 Average
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cell (--)
Specification
--
Blocking step
(agent) for 1 minute(s) · Concentration: 1µg/mL
Fixative
None
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Eyen SE

Verified customer

提交于 Aug 18 2022

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