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重组

重组Anti-SARS-CoV-2 Spike Glycoprotein S1抗体[HL6] - BSA and Azide free (ab281311)

Submit a review Submit a question References (1)
Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281311)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281311)
  • Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)
  • Sandwich ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281311)
  • Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)
  • Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)
  • Flow Cytometry - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)
  • Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [HL6] to SARS-CoV-2 Spike Glycoprotein S1 - BSA and Azide free
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, Indirect ELISA, Sandwich ELISA

概述

  • 产品名称

    Anti-SARS-CoV-2 Spike Glycoprotein S1抗体[HL6] - BSA and Azide free
    参阅全部 SARS-CoV-2 Spike Glycoprotein S1 一抗

  • 描述

    兔单克隆抗体[HL6] to SARS-CoV-2 Spike Glycoprotein S1 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, WB, IHC-P, Flow Cyt, Indirect ELISA, Sandwich ELISAmore details

  • 种属反应性

    与反应: SARS-CoV-2

  • 免疫原

    Recombinant fragment within Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. SARS-CoV-2 (strain Wuhan-Hu-1)

  • 阳性对照

    • IHC-P: SARS-CoV-2 (COVID-19) infected human placenta an human lung tissue. Flow cyt: SARS-CoV-2 (2019-nCoV) Spike 293T cells. WB: SARS-CoV-2 Spike transfected 293T cells. ICC/IF: SARS-CoV-2 Spike transfected 293T cells. ELISA: Recombinant full-length SARS-CoV-2 spike protein, SARS-CoV-2 spike (full length) transfected 293T whole cell extracts.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab281311于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/100 - 1/1000.
WB
1/1000 - 1/10000.
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
1/50.
Indirect ELISA
Use a concentration of 1 µg/ml.
Sandwich ELISA
Use a concentration of 1 µg/ml.
说明
ICC/IF
1/100 - 1/1000.
WB
1/1000 - 1/10000.
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
1/50.
Indirect ELISA
Use a concentration of 1 µg/ml.
Sandwich ELISA
Use a concentration of 1 µg/ml.

图片

  • Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)
    Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)
    All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311) at 1/5000 dilution

    Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : SARS-CoV Spike transfected 293T whole cell extrac
    Lane 3 : SARS-CoV-2 (COVID-19) Spike transfected 293T whole cell extract
    Lane 4 : MERS Spike transfected 293T whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody


    5% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281311)

    Paraffin embedded SARS-CoV-2 (COVID-19) infected human placenta (left) and normal human placenta (right) tissue stained for SARS-CoV-2 (COVID-19) nucleocapsid protein using ab281311 in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281311)

    Paraffin embedded SARS-CoV-2 (COVID-19) infected human lung tissue stained for SARS-CoV-2 (COVID-19) nucleocapsid protein using ab281311 in immunohistochemical analysis.

  • Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)
    Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)

    Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein (50 ng). Coated protein probed with ab281311 (2.4*10⁻⁶ ~ 20 nM). HRP-conjugated Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody.
    EC50: 0.251 nM

  • Sandwich ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)
    Sandwich ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)

    Sandwich ELISA detection of non-transfected and SARS-CoV-2 spike (full length) transfected 293T whole cell extracts using 5 µg/mL of capture antibody and ab281311 as detection antibody at 1 µg/mL. HRP-conjugated Rabbit IgG antibody at 1/10000 dilution was used to detect the primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281311)

    Mock and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block. SARS-CoV-2 (COVID-19) Spike S1 stained by ab281311 at 1/1000 dilution in immunohistochemical analysis. Fluoroshield with DAPI (blue).

    Antigen Retrieval was achieved with Citrate buffer at pH 6.0 for 15 minutes.

  • Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)
    Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)
    Mock and SARS-CoV-2 Spike-transfected 293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) spike stained (green) by ab281311 at 1/1000 dilution in Immunocytochemical analysis. Fluoroshield with DAPI (blue).
  • Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)
    Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)

    Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein or recombinant SARS-CoV spike S1 subunit protein (62.5-4000 ng/mL). Coated protein probed with ab281311 at 1 µg/mL. HRP-conjugated Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody.

  • Flow Cytometry - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)
    Flow Cytometry - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)

    ab281311 detects SARS-CoV-2 (COVID-19) spike protein by flow cytometry analysis.  SARS-CoV-2 (2019-nCoV) Spike 293T cells. Unlabelled sample was used as a control (blue) and ab281311 at 1/50 dilution (red). Acquisition of 20,000 events were collected for flow cytometry analysis.

  • Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)
    Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311)
    All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL6] - BSA and Azide free (ab281311) at 1/5000 dilution

    Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : SARS-CoV-2 (COVID-19) Spike transfected 293T whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody


    5% SDS-PAGE

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (1)

发表研究结果有使用 ab281311？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab281311 被引用在 1 文献中.

  • Kamel M  et al. Non-Invasive Detection of SARS-CoV-2 Antigen in Saliva versus Nasopharyngeal Swabs Using Nanobodies Conjugated Gold Nanoparticles. Trop Med Infect Dis 7:N/A (2022). PubMed: 35736981

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