重组Anti-SARS-CoV-2 Spike Glycoprotein S1抗体[HL263] - BSA and Azide free (ab281310)

Sandwich ELISA detection of non-transfected and SARS-CoV-2 spike (full length) transfected 293T whole cell extracts using SARS-CoV-2 (COVID-19) Spike S1 antibody as capture antibody at concentration of 5 μg/mL and ab281310 as detection antibody at concentration of 1 μg/mL. HRP-conjugated Rabbit IgG antibody was diluted at 1/10000 and used to detect the primary antibody.

Mock and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block. SARS-CoV-2 (COVID-19) Spike S1 stained by ab281310 (green) at 1/1000 dilution in immunohistochemical analysis. Fluoroshield with DAPI (blue). Antigen Retrieval was achieved with Citrate buffer at pH 6.0 for 15 minutes.

Mock and SARS-CoV-2 (COVID-19) Spike transfected 293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) Spike stained by ab281310 (green) at 1/500 dilution in immunocytochemical analysis. Fluoroshield with DAPI (blue).

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein (50 ng). Coated protein probed with ab281310 (2.4*10⁻⁶ ~ 20 nM). HRP-conjugated Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody.
EC50: 0.365 nM

Indirect ELISA analysis was performed by coating plate with 50 µL of recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein, and SARS-CoV spike S1 subunit protein at concentrations ranging from 0.0625 µg/mL to 4 µg/mL. The coated protein is detected with ab281310 at 1 µg/mL. HRP-conjugated Rabbit IgG antibody at 1/10000 dilution was used to detect the primary antibody.

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody as capture antibody at concentration of 5 µg/mL and ab281310 as detection antibody at concentration of 1 µg/mL. HRP-Rabbit IgG antibody was at 1/10000 dilution and used to detect the primary antibody.

5% SDS-PAGE