重组Anti-SARS-CoV-2 Spike Glycoprotein S1抗体[HL257] - BSA and Azide free (ab281306)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)](https://www.abcam.cn/ps/products/281/ab281306/Images/ab281306-1-SARSCoV2-COVID19-Spike-RBD-antibody-HL257-immunohistochemistry-normal-and-infected-placenta-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [HL257] to SARS-CoV-2 Spike Glycoprotein S1 - BSA and Azide free
- Suitable for: Indirect ELISA, IHC-P, WB, ICC
概述
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产品名称
Anti-SARS-CoV-2 Spike Glycoprotein S1抗体[HL257] - BSA and Azide free
参阅全部 SARS-CoV-2 Spike Glycoprotein S1 一抗 -
描述
兔单克隆抗体[HL257] to SARS-CoV-2 Spike Glycoprotein S1 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: Indirect ELISA, IHC-P, WB, ICCmore details -
种属反应性
与反应: SARS-CoV-2 -
免疫原
Synthetic peptide within Human coronavirus SARS-CoV-2 Spike Glycoprotein S1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Strain Wuhan-Hu-1.
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阳性对照
- IHC-P: SARS-CoV-2 (COVID-19) infected mouse lung, human lung and placenta tissue. ICC: SARS-CoV-2 (COVID-19) Spike transfected HEK-293T cells. WB: SARS-CoV-2 (COVID-19) Spike transfected HEK-293T whole cell extracts, SARS-CoV-2 (COVID-19) Spike RBD protein. ELISA: Recombinant SARS-CoV-2 (COVID-19) Spike RBD protein.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.4
Constituent: 100% PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
HL257 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab281306于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Indirect ELISA |
Use at an assay dependent concentration.
|
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| WB |
1/1000 - 1/10000.
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| ICC |
1/100 - 1/1000.
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| 说明 |
|---|
|
Indirect ELISA
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/10000. |
|
ICC
1/100 - 1/1000. |
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)Paraffin-embedded non-infected (left) and infected (right) human placenta tissue stained for SARS-CoV-2 (COVID-19) Spike RBD using ab281306 at 1/500 dilution in immunohistochemical analysis. Antigen Retrieval was achieved with Citrate buffer at pH 6.0 for 20 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)](https://www.abcam.cn/ps/products/281/ab281306/Images/ab281306-2-SARSCoV2-COVID19-Spike-RBD-antibody-HL257-immunohistochemistry-normal-and-infected-lung-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)Paraffin-embedded non-infected (left) and infected (right) human lung tissue stained for SARS-CoV-2 (COVID-19) Spike RBD using ab281306 at 1/1000 dilution in immunohistochemical analysis. Antigen Retrieval was achieved with Citrate buffer at pH 6.0 for 20 minutes. -
![Immunocytochemistry - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)](https://www.abcam.cn/ps/products/281/ab281306/Images/ab281306-3-SARSCoV2-COVID19-Spike-RBD-antibody-HL257-immunohistochemistrycellpellet-293tcells.jpg)
Immunocytochemistry - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)Mock and SARS-CoV-2 (COVID-19) Spike transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell FFPE Cell Pellet Block. SARS-CoV-2 (COVID-19) Spike RBD stained by ab281306 (green) at 1/1000 dilution in immunocytochemical analysis. Fluoroshield with DAPI (blue). Antigen Retrieval achieved with Citrate buffer at pH 6.0 for 15 minutes.
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![Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)](https://www.abcam.cn/ps/products/281/ab281306/Images/ab281306-4-SARSCoV2-COVID19-Spike-RBD-antibody-HL257-westernblot-293tcells.jpg)
Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : Transfected HEK-293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody5% SDS-PAGE
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![Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)](https://www.abcam.cn/ps/products/281/ab281306/Images/ab281306-5-SARSCoV2-COVID19-Spike-RBD-antibody-HL257-indirectelisa.jpg)
Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) protein (62.5-4000 ng/mL). Coated protein probed ab281306 at 1 µg/mL. HRP-Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody.
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![Immunocytochemistry - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)](https://www.abcam.cn/ps/products/281/ab281306/Images/ab281306-6-SARSCoV2-COVID19-Spike-RBD-antibody-HL257-immunocytochemistry-293tcells.jpg)
Immunocytochemistry - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)Mock and transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) Spike S1 stained (green) by ab281306 at 1/1000 dilution in immunocytochemical analysis. Fluoroshield with DAPI (blue).
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![Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)](https://www.abcam.cn/ps/products/281/ab281306/Images/ab281306-9-SARSCoV2-COVID19Spike-RBD-antibody-HL257-westernblot-293Tcells.jpg)
Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : SARS-CoV Spike transfected HEK-293T whole cell extracts
Lane 3 : SARS-CoV Spike (COVID-19) transfected HEK-293T whole cell extracts
Lane 4 : MERS-CoV Spike transfected HEK-293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody5% SDS-PAGE
-
![Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)](https://www.abcam.cn/ps/products/281/ab281306/Images/ab281306-456922-SARSCoV2-COVID19-Spike-RBD-antibody-HL257-wb.JPG)
Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306) at 1/20000 dilution + SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active)
Secondary
HRP-conjugated anti-rabbit IgG antibody12% SDS-PAGE
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![Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)](https://www.abcam.cn/ps/products/281/ab281306/Images/ab281306-8-SARSCoV2-COVID19-Spike-RBD-antibody-HL257-westernblot-veroe6cells.jpg)
Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306) at 1/5000 dilution
Lane 1 : Non-transfected Vero E6 whole cell extracts
Lane 2 : SARS-CoV-2 (Covid-19) transfected Vero E6 whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody5% SDS-PAGE
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)](https://www.abcam.cn/ps/products/281/ab281306/Images/ab281306-7-SARSCoV2-COVID19-Spike-RBD-antibody-HL257-immunohistochemistry-infected-lung-tissue-mouse-model.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL257] - BSA and Azide free (ab281306)Paraffin-embedded infected lung tissue derived from the AdV-hACE2-transduced BALB/c mice model. SARS-CoV-2 (COVID-19) Spike RBD protein stained by IgG control (left) and ab281306 at 1/1000 dilution (right) in immunohistochemical analysis. Antigen Retrieval was achieved with Citrate buffer at pH 6.0 for 20 minutes.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab281306 被引用在 1 文献中.
- Beck A et al. Emergence of SARS-CoV-2 spike protein at the vaccination site. Immun Inflamm Dis 11:e827 (2023). PubMed: 36988249
