Anti-SARS-CoV-2 Spike Glycoprotein S1 [8B12-C2] – Human IgG1 (Chimeric)

• I-ELISA

Lab

Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 [8B12-C2] – Human IgG1 (Chimeric) (AB322272)

Indirect ELISA showing primary antibody ab322272 binding to Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, ab272105). Plates were coated with Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, ab272105) and Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera, ab272106) at 1000 ng/ml. Binding of ab322272 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution). Secondary antibody, Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) was used at 1 : 5000.

• WB

Lab

Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 [8B12-C2] – Human IgG1 (Chimeric) (AB322272)

False colour image of Western blot : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - Human IgG1 (Chimeric) (ab322272) staining at 1/500 dilution, shown in black; Mouse Monoclonal 6X His tag® (ab18184) loading control staining at 1/200 dilution, shown in red. In Western blot, ab322272 was shown to bind specifically to SARS-CoV and SARS-CoV-2 Spike Glycoproteins. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1 : 10000 and Goat anti-Mouse IgG H&L 680RD at 1 : 20000.

All lanes:

Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 [8B12-C2] – Human IgG1 (Chimeric) (ab322272) at 1/500 dilution

Lane 1:

Western blot - Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) (<a href='/products/proteins-peptides/recombinant-human-coronavirus-sars-cov-2-spike-glycoprotein-s1-active-ab273068'>ab273068</a>) at 0.2 µg

Lane 2:

SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Recombinant Protein at 0.2 µg

Lane 3:

SARS-CoV Spike S1+S2 ECD-His Recombinant Protein at 0.2 µg

Secondary

Lanes 1 - 3:

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-human-igg-fc-hrp-preadsorbed-ab98624'>ab98624</a>) at 1/10000 dilution

Lanes 1 - 3:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 120-160 kDa

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Exposure time: 5s