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重组

重组Anti-SARS-CoV-2 spike glycoprotein抗体[H6] -人IgG1 (Chimeric) (ab272854)

Reviews (3) Submit a question References (2)
Western blot - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)
  • ELISA - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)
  • ELISA - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)
  • Functional Studies - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)
  • Functional Studies - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)
  • Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Human monoclonal [H6] to SARS-CoV-2 spike glycoprotein - Chimeric
  • Suitable for: ELISA, WB, Functional Studies

Conjugates logo Related conjugates and formulations

Biotin FITC HRP

概述

  • 产品名称

    Anti-SARS-CoV-2 spike glycoprotein抗体[H6] -人IgG1 (Chimeric)
    参阅全部 SARS-CoV-2 Spike Glycoprotein 一抗

  • 描述

    人单克隆抗体[H6] to SARS-CoV-2 spike glycoprotein - Chimeric

  • 宿主

    Human

  • 特异性

    We have in-house data that suggests this product also reacts with SARS-CoV-1 spike protein in Western blot.

  • 经测试应用

    适用于: ELISA, WB, Functional Studiesmore details

  • 种属反应性

    与反应: SARS-CoV, SARS-CoV-2

  • 免疫原

    Recombinant fragment corresponding to Human SARS-CoV-2 spike glycoprotein aa 16-685.
    Database link: P0DTC2

  • 阳性对照

    • WB: SARS-Cov1 spike protein transfected Expi cell lysate, SARS-Cov1 3xFlag spike protein transfected Expi cell lysate, SARS-Cov2 spike protein transfected Expi cell lysate and SARS-Cov2 3xFlag spike protein transfected Expi cell lysate.

  • 常规说明

    Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854) is a recombinant human/mouse chimeric monoclonal antibody consisting of Mouse scFv fused with human IgG1.

    As the Fc region is human, an anti-Human secondary antibody should be used for detection. A suitable anti-Mouse secondary may also be used.

    Applications overview

    Tick: Tested and Guaranteed to work      X: Will not work      —: No data

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.40
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...

  • 纯度

    >90% by SDS-PAGE

  • 纯化说明

    Screened the positive clones from mouse ScFv antibody libraries, then expressed in 293F cells.

  • 克隆

    单克隆

  • 克隆编号

    H6

  • 同种型

    IgG1

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab272854于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA (1)
1/10000 - 1/50000.
WB
Use a concentration of 1 µg/ml.
Functional Studies
Use at an assay dependent concentration.
说明
ELISA
1/10000 - 1/50000.
WB
Use a concentration of 1 µg/ml.
Functional Studies
Use at an assay dependent concentration.

图片

  • Western blot - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)
    Western blot - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)
    All lanes : Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854) at 1 µg/ml

    Lane 1 : Mock transfected Expi cell lysate
    Lane 2 : PDL-1 Flag transfected Expi cell lysate
    Lane 3 : SARS-Cov1 spike protein transfected Expi cell lysate
    Lane 4 : SARS-Cov1 3xFlag spike protein transfected Expi cell lysate
    Lane 5 : SARS-Cov2 spike protein transfected Expi cell lysate
    Lane 6 : SARS-Cov2 3xFlag spike protein transfected Expi cell lysate
    Lane 7 : MERS Spike protein transfected Expi cell lysate
    Lane 8 : MERS 3xFlag Spike protein transfected Expi cell lysate

    Lysates/proteins at 10 µg per lane.

    Performed under reducing conditions.

    Observed band size: 200 kDa why is the actual band size different from the predicted?



    Lanes 1 - 8: Merged signal (red and green). Green - ab272854 observed at 200 kDa. Red - loading control.

    ab272854 was shown to react with SARS-CoV-2 spike glycoprotein in western blot. Membranes were blocked in 3% milk before incubation with ab272854 overnight at 4°C at 1 ug/ml. Blots were incubated with Donkey anti-Mouse IgG H&L(IRDye® 800CW) preabsorbed (ab216774) antibody at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    Note: ab216774 is suitable for the recognition of the Mouse scFv fragment of ab272854.

  • ELISA - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)
    ELISA - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)

    Binding Activity of ab272854 with SARS-CoV-2 spike glycoprotein.
    Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 spike glycoprotein at 2 μg/ml can bind ab272854. The EC50 of ab272854 is 36.79-48.87 ng/ml.            

  • ELISA - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)
    ELISA - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)

    Binding Activity of ab272854 with SARS-CoV-2-S1-RBD
    Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind ab272854, the EC50 of SARS-CoV-2-S Antibody is 19.60-39.42 ng/ml.

  • Functional Studies - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)
    Functional Studies - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)

    Binding signal of Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854) and SARS-CoV-2-S1-RBD was inhibited by ACE2 protein-HRP conjugated inhibitor with the IC50 23.32 nM.

  • Functional Studies - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)
    Functional Studies - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)

    Binding signal of Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854) and SARS-CoV-2-S1-RBD was inhibited by ACE2 protein-HRP conjugated inhibitor with the IC50 2.38 µg/mL.

  • Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)
    Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (2)

发表研究结果有使用 ab272854？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab272854 被引用在 2 文献中.

  • Boix-Besora A  et al. Gag Virus-like Particles Functionalized with SARS-CoV-2 Variants: Generation, Characterization and Recognition by COVID-19 Convalescent Patients' Sera. Vaccines (Basel) 11:N/A (2023). PubMed: 38005972
  • Boix-Besora A  et al. Optimization, Production, Purification and Characterization of HIV-1 GAG-Based Virus-like Particles Functionalized with SARS-CoV-2. Vaccines (Basel) 10:N/A (2022). PubMed: 35214708

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

ELISA abreview for Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric)

 Good
Abreviews
abreview image
Application
ELISA
Sample
Mouse Recombinant protein (COVID Spike)
Specification
COVID Spike
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: 21°C
Type
Sandwich (Capture)
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Aslihan Turhan

Verified customer

提交于 Aug 17 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric)

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
SARS-CoV-2 Cell (VERO-E6 (african green monkey))
Permeabilization
Yes - 0.5% Saponin
Specification
VERO-E6 (african green monkey)
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Harry Wodrich

Verified customer

提交于 Jul 20 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric)

 Inconclusive
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (SARS-CoV-2 PCR positive human Lung section)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH6 NaCitrate buffer
Permeabilization
No
Specification
SARS-CoV-2 PCR positive human Lung section
Blocking step
H2O2 as blocking agent for 7 minute(s) · Concentration: 3% · Temperature: 24°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

In Hwa Um

Verified customer

提交于 Jun 29 2020

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