Anti-SARS-CoV-2 spike glycoprotein抗体- Coronavirus (ab272504)
Key features and details
- Rabbit polyclonal to SARS-CoV-2 spike glycoprotein - Coronavirus
- Suitable for: IHC-P, ICC/IF, ELISA, WB
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-SARS-CoV-2 spike glycoprotein抗体- Coronavirus
参阅全部 SARS-CoV-2 Spike Glycoprotein 一抗 -
描述
兔多克隆抗体to SARS-CoV-2 spike glycoprotein - Coronavirus -
宿主
Rabbit -
特异性
The immunogen for ab272504 is within the last 50 aa of the spike protein - a peptide corresponding to 20 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19) Spike glycoprotein. The Extracellular domain (ECD) is from aa 1 to 1208 (full length 1273aa). Therefore, this antibody detects the transmembrane and cytoplasm domains at the C terminus, but does not detect the ECD (which is the region expressed in many commercially available spike proteins). ab272504 can be used for the detection of full length spike protein and spike protein in COVID-19 patient samples. It will detect 4 ng of free peptide at 1 μg/mL.
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经测试应用
适用于: IHC-P, ICC/IF, ELISA, WBmore details -
种属反应性
与反应: SARS-CoV-2 -
免疫原
Synthetic peptide within Human coronavirus SARS-CoV-2 Spike Glycoprotein (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. 20 amino acid peptide within the last 50 amino acids of the SARS CoV-2 spike glycoprotein
Database link: QHD43416
(Peptide available asab273063) -
阳性对照
- WB: HEK-293 cells overexpressing SARS-CoV-2 spike glycoprotein, cell lysate. ICC: HEK-293 cells overexpressing SARS-CoV-2 spike glycoprotein. IHC-P: COVID-19 patient lung tissue.
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常规说明
Applications overview
Tick: Tested and Guaranteed to work X: Will not work —: No data
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Immunizing Peptide (Blocking)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272504于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
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| IHC-P | (3) |
Use a concentration of 0.2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| ELISA |
Use a concentration of 1 µg/ml.
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| WB |
Use a concentration of 1 µg/ml. Can be blocked with Human coronavirus SARS-CoV-2 spike glycoprotein peptide (ab273063).
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| 说明 |
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IHC-P
Use a concentration of 0.2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 1 µg/ml. |
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ELISA
Use a concentration of 1 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Can be blocked with Human coronavirus SARS-CoV-2 spike glycoprotein peptide (ab273063). |
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504)This image is courtesy of an anonymous AbreviewImmunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of Covid-19 positive lung, autopsy sample tissue. Stained with ab272504 at 1/200 dilution. Blocking was done with Nanostring Buffer W for 1 hour at 25°C. The sample was incubated with the primary antibody and Nanostring Buffer W at 12µg/ml for 12 hours at 4°C. Antigen retrieval method was heat mediated, Citrate Buffer pH 6.0.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504)This image is courtesy of an anonymous AbreviewImmunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of paraformaldehyde fixed human Skin (Covid-19 positive, autopsy sample) tissue. Stained with ab272504 at 1/200 dilution. Secondary antibody used was HRP Novolink Max Polymer Detection System. Blocking was done with 10% milk for 30 minutes at 21°C. The sample was incubated with the primary antibody and 1% BSA for 12 hours at 4°C. Antigen retrieval method was heat mediated, Dako Retrieval Solution (S1699).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504)Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (ab272504, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in macrophages and airway epithelium of COVID-19 patient lung but not in non-COVID-19 patient lung.
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Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504)Immunofluorescent analysis of 4% paraformaldehyde-fixed 293 spike transfected cells labelling SARS-CoV-2 (Covid-19) Spike with ab272504 at 1 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Western blot - Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504)All lanes : Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504) at 1 µg/ml
Lane 1 : Wild-type HEK-293 cell lysate
Lane 2 : HEK-293 overexpressing SARS-CoV-2 spike glycoprotein, cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilutionPrimary incubation for 1 hour at room temperature.
Blocking and dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504)Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (ab272504, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in macrophages of COVID-19 patient lung.
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ELISA - Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504)A direct ELISA was performed using antigen or control peptide as coating antigen and ab272504 as the capture antibody at 1 μg/ml, followed by the secondary antibody goat anti-rabbit IgG HRP conjugate at 1/20000 dilution. Detection range was from 0.5 ng/ml to 1000 ng/ml.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (48)
ab272504 被引用在 48 文献中.
- Kulasinghe A et al. Transcriptomic profiling of cardiac tissues from SARS-CoV-2 patients identifies DNA damage. Immunology 168:403-419 (2023). PubMed: 36107637
- Bogard G et al. SARS-CoV-2 infection induces persistent adipose tissue damage in aged golden Syrian hamsters. Cell Death Dis 14:75 (2023). PubMed: 36725844
- Wang R et al. SARS-CoV-2 restructures host chromatin architecture. Nat Microbiol 8:679-694 (2023). PubMed: 36959507
- Popov H et al. Modified Banff Criteria in Assessing SARS-CoV-2-Associated Renal Pathology: An Autopsy Study. Cureus 15:e37260 (2023). PubMed: 37168215
- Boluda S et al. Golgi localization of SARS-CoV-2 spike protein and interaction with furin in cerebral COVID-19 microangiopathy: a clue to the central nervous system involvement? Free Neuropathol 4:N/A (2023). PubMed: 37283933