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重组

重组Anti-SARS-CoV-2 nucleocapsid蛋白抗体[HL5511] - BSA and Azide free (ab281302)

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Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302)
  • Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302)
  • Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302)
  • Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302)
  • Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302)
  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302)
  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [HL5511] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free
  • Suitable for: IHC-P, Indirect ELISA, WB, ICC, Sandwich ELISA

概述

  • 产品名称

    Anti-SARS-CoV-2 nucleocapsid蛋白抗体[HL5511] - BSA and Azide free
    参阅全部 SARS-CoV-2 nucleocapsid protein 一抗

  • 描述

    兔单克隆抗体[HL5511] to SARS-CoV-2 nucleocapsid蛋白- BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, Indirect ELISA, WB, ICC, Sandwich ELISAmore details

  • 种属反应性

    与反应: SARS-CoV-2

  • 免疫原

    Recombinant full length protein corresponding to Human coronavirus SARS-CoV-2 nucleocapsid protein. Strain Wuhan-Hu-1

  • 阳性对照

    • WB: SARS-CoV-2 (COVID-19) Nucleocapsid transfected Vero E6 cells, SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cells. ICC: SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cells. IHC-P: SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cells. ELISA: Recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab281302于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Indirect ELISA
Use a concentration of 1 µg/ml.
WB
1/1000 - 1/10000.
ICC
1/100 - 1/1000.
Sandwich ELISA
Use a concentration of 5 µg/ml.
说明
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Indirect ELISA
Use a concentration of 1 µg/ml.
WB
1/1000 - 1/10000.
ICC
1/100 - 1/1000.
Sandwich ELISA
Use a concentration of 5 µg/ml.

靶标

  • 相关性

    Nucleocapsid protein is a most abundant protein of coronavirus on the helical nucleocapsid of coronaviruses. N protein of SARS CoV-2 ab273530 is a structural protein required for RNA synthesis, and has RNA chaperone activity that may be involved in template switch. N protein enters the host cell with the viral RNA to facilitate its replication and process the virus particle assembly and release. N protein is a highly immunogenic phosphoprotein also implicated in modulating cell signalling pathways. Coronavirus nucleocapsid proteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein.

  • 别名

    • 2019-nCoV nucleocapsid protein antibody
    • N antibody
    • NC antibody
    • Nucleoprotein antibody
    • Protein N antibody
    see all

图片

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302)
    All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302) at 1/5000 dilution

    Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : SARS-CoV NP transfected 293T whole cell extracts
    Lane 3 : SARS-CoV-2 (COVID-19) NP transfected 293T whole cell extracts
    Lane 4 : MERS-CoV NP transfected 293T whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302)

    Mock and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell FFPE Cell Pellet Block. SARS-CoV-2 (COVID-19) nucleocapsid stained by ab281302 (green) at 1/1000 dilution immunohistochemical analysis. Fluoroshield with DAPI (blue). Antigen Retrieval was achieved with Citrate buffer at pH 6.0 for 15 minutes.

  • Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302)
    Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302)

    Mock and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) nucleocapsid stained by ab281302 (green) at 1/1000 dilution immunocytochemical analysis. Fluoroshield with DAPI (blue).

  • Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302)
    Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302)

    Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (50 ng). Coated protein probed with ab281302 (4.8*10⁻⁶ ~ 20 nM). HRP-conjugated Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody.
    EC50: 0.014 nM

  • Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302)
    Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302)

    Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (1000-15.63 ng/mL). Coated protein probed with ab281302 at 1 µg/mL. HRP-Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody.

  • Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302)
    Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302)

    Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein using ab281302 as capture antibody at concentration of 5 µg/mL and a HRP-conjugated ab281295 as detection antibody at concentration of 1 µg/mL.

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302)
    All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302) at 1/5000 dilution

    Lane 1 : Vero E6 whole cell extracts
    Lane 2 : SARS-CoV-2 nucleocapsid transfected Vero E6 whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody
  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302)
    All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5511] - BSA and Azide free (ab281302) at 1/5000 dilution

    Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : SARS-CoV-2 nucleocapsid transfected 293T whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

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