重组Anti-SARS-CoV-2 nucleocapsid蛋白抗体[HL5410] - BSA and Azide free (ab281301)
![Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)](https://www.abcam.cn/ps/products/281/ab281301/Images/ab281301-456639-SARSCoV2-nucleocapsid-protein-antibody-HL5410-wb.JPG "Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [HL5410] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free
- Suitable for: WB, ICC, Sandwich ELISA, Indirect ELISA, IHC-P
概述
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产品名称
Anti-SARS-CoV-2 nucleocapsid蛋白抗体[HL5410] - BSA and Azide free
参阅全部 SARS-CoV-2 nucleocapsid protein 一抗 -
描述
兔单克隆抗体[HL5410] to SARS-CoV-2 nucleocapsid蛋白- BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WB, ICC, Sandwich ELISA, Indirect ELISA, IHC-Pmore details -
种属反应性
与反应: SARS-CoV-2 -
免疫原
Recombinant full length protein corresponding to Human coronavirus SARS-CoV-2 nucleocapsid protein. Strain Wuhan-Hu-1
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阳性对照
- WB: SARS-CoV Nucleocapsid transfected 293T whole cell extracts, SARS-CoV Nucleocapsid transfected Vero E6 cells. ICC: SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cells. IHC-P: SARS-CoV Nucleocapsid transfected 293T cells. ELISA: Recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.4
Constituent: 100% PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
HL5410 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab281301于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000.
|
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| ICC |
1/1000.
|
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| Sandwich ELISA |
Use a concentration of 5 µg/ml.
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| Indirect ELISA |
Use a concentration of 1 µg/ml.
|
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| IHC-P |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. |
|
ICC
1/1000. |
|
Sandwich ELISA
Use a concentration of 5 µg/ml. |
|
Indirect ELISA
Use a concentration of 1 µg/ml. |
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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相关性
Nucleocapsid protein is a most abundant protein of coronavirus on the helical nucleocapsid of coronaviruses. N protein of SARS CoV-2 ab273530 is a structural protein required for RNA synthesis, and has RNA chaperone activity that may be involved in template switch. N protein enters the host cell with the viral RNA to facilitate its replication and process the virus particle assembly and release. N protein is a highly immunogenic phosphoprotein also implicated in modulating cell signalling pathways. Coronavirus nucleocapsid proteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. -
别名
- 2019-nCoV nucleocapsid protein antibody
- N antibody
- NC antibody
see all
图片
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Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301) at 1/5000 dilution
Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) extracts
Lane 2 : SARS-CoV NP transfected 293T whole cell extracts
Lane 3 : SARS-CoV-2 (COVID-19) transfected 293T whole cell extracts
Lane 4 : MERS-CoV NP transfected 293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281301)Mock and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell FFPE Cell Pellet Block. SARS-CoV-2 (COVID-19) nucleocapsid stained (green) by ab281301 at 1/1000 dilution in immunohistochemical analysis. Fluoroshield with DAPI (blue). Antigen Retrieval was achieved with Citrate buffer at pH 6.0 for 15 minutes.
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![Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)](https://www.abcam.cn/ps/products/281/ab281301/Images/ab281301-5-SARSCoV2-COVID19-nucleocapsid-antibody-HL5410-immunocytochemistry-293tcells.jpg)
Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)Mock and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) nucleocapsid stained (green) by ab281301 at 1/1000 dilution in immunocytochemical analysis. Fluoroshield with DAPI (blue).
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![Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)](https://www.abcam.cn/ps/products/281/ab281301/Images/ab281301-457465-SARSCoV2-nucleocapsid-protein-antibody-HL5410-elisa-3.JPG)
Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)Sandwich ELISA detection of non-transfected and SARS-CoV-2, MERS-CoV and SARS-CoV Nucleocapsid transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts, SARS-CoV OC43 lysate, SARS-CoV NL63 lysate, or SARS-CoV 229E lysate using ab281301 as capture antibody at concentration of 5 μg/mL and a SARS-CoV-2 (COVID-19) nucleocapsid antibody as detection antibody at concentration of 1 μg/mL. HRP-conjuagted mouse IgG antibody was diluted at 1/10000 dilution and used to detect the primary antibody.
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![Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)](https://www.abcam.cn/ps/products/281/ab281301/Images/ab281301-1-SARSCoV2-COVID19-nucleocapsid-antibody-HL5410-sandwichelisa.jpg)
Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein using ab281301 as capture antibody at concentration of 5 µg/mL and a HRP-conjugated ab281299 as detection antibody at concentration of 1 µg/mL.
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![Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)](https://www.abcam.cn/ps/products/281/ab281301/Images/ab281301-3-SARSCoV2-COVID19-nucleocapsid-antibody-HL5410-indirectelisa.jpg)
Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (1000-15.63 ng/mL). Coated protein probed with ab281301 at 1 µg/mL. HRP-conjugated Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody.
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![Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)](https://www.abcam.cn/ps/products/281/ab281301/Images/ab281301-6-SARSCoV2-COVID19-nucleocapsid-antibody-HL5410-westernblot-293tcells.jpg)
Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301) at 1/5000 dilution
Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
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![Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)](https://www.abcam.cn/ps/products/281/ab281301/Images/ab281301-2-SARSCoV2-COVID19-nucleocapsid-antibody-HL5410-westernblot-veroe6cells.jpg)
Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301) at 1/5000 dilution
Lane 1 : Vero E6 whole cell extracts
Lane 2 : SARS-CoV-2 nucleocapsid transfected Vero E6 whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
-
![Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)](https://www.abcam.cn/ps/products/281/ab281301/Images/ab281301-457463-SARSCoV2-nucleocapsid-protein-antibody-HL5410-elisa.JPG)
Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein using ab281301 as capture antibody at concentration of 5 μg/mL and a HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody as detection antibody at concentration of 1 μg/mL.
-
![Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)](https://www.abcam.cn/ps/products/281/ab281301/Images/ab281301-457464-SARSCoV2-nucleocapsid-protein-antibody-HL5410-elisa2.JPG)
Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL5410] - BSA and Azide free (ab281301)Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein using ab281301 as capture antibody at concentration of 5 μg/mL and a HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody as detection antibody at concentration of 1 μg/mL.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab281301 尚未被引用在任何文献中。
