Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-SARS-CoV-2 nucleocapsid蛋白抗体[HL455-MS] - BSA and Azide free (ab281300)

Submit a review Submit a question References (2)
Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
  • Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
  • Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)

Key features and details

  • Mouse monoclonal [HL455-MS] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free
  • Suitable for: WB, ICC, Sandwich ELISA, IHC-P
  • Isotype: IgG1

概述

  • 产品名称

    Anti-SARS-CoV-2 nucleocapsid蛋白抗体[HL455-MS] - BSA and Azide free
    参阅全部 SARS-CoV-2 nucleocapsid protein 一抗

  • 描述

    小鼠单克隆抗体[HL455-MS] to SARS-CoV-2 nucleocapsid蛋白- BSA and Azide free

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, ICC, Sandwich ELISA, IHC-Pmore details

  • 种属反应性

    与反应: SARS-CoV-2

  • 免疫原

    Recombinant full length protein corresponding to Human coronavirus SARS-CoV-2 nucleocapsid protein. Strain Wuhan-Hu-1.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • IHC-P: HEK-293T (transfected with -SARS-CoV-2 nucleocapsid protein) cell pellet block. ICC: HEK-293T cells transfected with -SARS-CoV-2 nucleocapsid. WB: VeroE6 and HEK-293T transfected with SARS-CoV-2 nucleocapsid, whole cell extract.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab281300于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000.
ICC
1/100 - 1/1000.
Sandwich ELISA
Use a concentration of 1 µg/ml.
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
WB
1/1000 - 1/10000.
ICC
1/100 - 1/1000.
Sandwich ELISA
Use a concentration of 1 µg/ml.
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 相关性

    Nucleocapsid protein is a most abundant protein of coronavirus on the helical nucleocapsid of coronaviruses. N protein of SARS CoV-2 ab273530 is a structural protein required for RNA synthesis, and has RNA chaperone activity that may be involved in template switch. N protein enters the host cell with the viral RNA to facilitate its replication and process the virus particle assembly and release. N protein is a highly immunogenic phosphoprotein also implicated in modulating cell signalling pathways. Coronavirus nucleocapsid proteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein.

  • 别名

    • 2019-nCoV nucleocapsid protein antibody
    • N antibody
    • NC antibody
    • Nucleoprotein antibody
    • Protein N antibody
    see all

图片

  • Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
    Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)

    Mock and SARS-CoV-2 nucleocapsid transfected HEK 293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 nucleocapsid  (green) was labeled using ab281300 at 1/1000 dilution in immunocytochemical analysis. Fluoroshield with DAPI (blue).

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
    All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300) at 1/5000 dilution

    Lane 1 : 293T whole cell extracts (30 µg)
    Lane 2 : 293T whole cell extracts (30 µg) transfected with SARS-CoV NP
    Lane 3 : 293T whole cell extracts (30 µg) transfected with SARS-CoV-2 (COVID-19) NP
    Lane 4 : 293T whole cell extracts (30 µg) transfected with MERS-CoV NP

    Secondary
    All lanes : HRP-conjugated anti-mouse IgG antibody


    Upper panel probed with ab281300.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)

    Mock and SARS-CoV-2 Nucleocapsid transfected HEK-293T cell FFPE Cell Pellet Block. SARS-CoV-2 (COVID-19) nucleocapsid stained (green) by ab281300 at 1/1000 dilution in immunohistochemical analysis. Fluoroshield with DAPI (blue). Antigen Retrieval achieved with Citrate buffer at pH 6.0 for 15 minutes.

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
    All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300) at 1/5000 dilution

    All lanes : Vero E6 whole cell extracts (30 µg)

    Secondary
    Lane 1 : HRP-conjugated anti-mouse IgG antibody
    Lane 2 : HRP-conjugated anti-mouse IgG antibody transfected with SARS-CoV-2 nucleocapsid
  • Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
    Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)

    Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein using ab281301 as capture antibody at concentration of 5 µg/mL and ab281300 as detection antibody at concentration of 1 µg/mL. HRP-Mouse IgG antibody at 1/10000 dilution wasused to detect the primary antibody. Sensitivity: 2.68 pg/mL.

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
    All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300) at 1/5000 dilution

    Lane 1 : 293T whole cell extracts (30 µg)
    Lane 2 : 293T whole cell extracts (30 µg) transfected with SARS-CoV-2 nucleocapsid

    Secondary
    All lanes : HRP-conjugated anti-mouse IgG antibody
  • Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)
    Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL455-MS] - BSA and Azide free (ab281300)

    Sandwich ELISA detection of non-transfected and SARS-CoV-2 , MERS-CoV and SARS-CoV Nucleocapsid transfected HEK-293T whole cell extracts, SARS-CoV OC43 lysate, SARS-CoV NL63 lysate, or SARS-CoV 229E lysate using 281301 as capture antibody at concentration of 5 μg/mL and ab281300 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP)  was diluted at 1:10000 and used to detect the primary antibody.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (2)

发表研究结果有使用 ab281300？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab281300 被引用在 2 文献中.

  • López-Farfán D  et al. Exploring SARS-CoV-2 and Plasmodium falciparum coinfection in human erythrocytes. Front Immunol 14:1120298 (2023). PubMed: 36993979
  • Cacciottolo M  et al. Exosome-Based Multivalent Vaccine: Achieving Potent Immunization, Broadened Reactivity, and Strong T-Cell Responses with Nanograms of Proteins. Microbiol Spectr 11:e0050323 (2023). PubMed: 37093009

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

There are currently no Customer reviews or Questions for ab281300.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com