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重组

重组Anti-SARS-CoV-2 nucleocapsid蛋白抗体[HL453] - BSA and Azide free (ab281298)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL453] - BSA and Azide free (ab281298)
  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL453] - BSA and Azide free (ab281298)
  • Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL453] - BSA and Azide free (ab281298)
  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL453] - BSA and Azide free (ab281298)
  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL453] - BSA and Azide free (ab281298)
  • Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL453] - BSA and Azide free (ab281298)
  • Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL453] - BSA and Azide free (ab281298)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [HL453] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free
  • Suitable for: WB, ICC, Indirect ELISA, IHC-P

概述

  • 产品名称

    Anti-SARS-CoV-2 nucleocapsid蛋白抗体[HL453] - BSA and Azide free
    参阅全部 SARS-CoV-2 nucleocapsid protein 一抗

  • 描述

    兔单克隆抗体[HL453] to SARS-CoV-2 nucleocapsid蛋白- BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, ICC, Indirect ELISA, IHC-Pmore details

  • 种属反应性

    与反应: SARS-CoV-2

  • 免疫原

    Recombinant full length protein corresponding to Human coronavirus SARS-CoV-2 nucleocapsid protein. Strain strain Wuhan-Hu-1.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • WB: HEK-293T and VeroE6 transfected with SARS-CoV-2 (COVID-19) Nucleocapsid, whole cell extracts. ICC: HEK-293T cells transfected with SARS-CoV-2 (COVID-19) Nucleocapsid. IHC-P: HEK-293T (transfected with SARS-CoV-2 (COVID-19) Nucleocapsid) cell pellet block.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab281298于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000.
ICC
1/100 - 1/1000.
Indirect ELISA
Use a concentration of 1 µg/ml.
IHC-P
1/1000.
说明
WB
1/1000 - 1/10000.
ICC
1/100 - 1/1000.
Indirect ELISA
Use a concentration of 1 µg/ml.
IHC-P
1/1000.

靶标

  • 相关性

    Nucleocapsid protein is a most abundant protein of coronavirus on the helical nucleocapsid of coronaviruses. N protein of SARS CoV-2 ab273530 is a structural protein required for RNA synthesis, and has RNA chaperone activity that may be involved in template switch. N protein enters the host cell with the viral RNA to facilitate its replication and process the virus particle assembly and release. N protein is a highly immunogenic phosphoprotein also implicated in modulating cell signalling pathways. Coronavirus nucleocapsid proteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein.

  • 别名

    • 2019-nCoV nucleocapsid protein antibody
    • N antibody
    • NC antibody
    • Nucleoprotein antibody
    • Protein N antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL453] - BSA and Azide free (ab281298)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL453] - BSA and Azide free (ab281298)

    Mock and SARS-CoV-2 nucleocapsid  transfected HEK-293T cell FFPE Cell Pellet Block. SARS-CoV-2 (COVID-19) nucleocapsid stained (green) by ab281298 at 1/1000 dilution immunohistochemical analysis. Fluoroshield with DAPI (blue). Antigen Retrieval was achieved with Citrate buffer at pH 6.0 for 15 minutes.

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL453] - BSA and Azide free (ab281298)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL453] - BSA and Azide free (ab281298)
    All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL453] - BSA and Azide free (ab281298) at 1/5000 dilution

    Lane 1 : HEK-293T whole cell extracts (30 µg) non-transfected
    Lane 2 : HEK-293T whole cell extracts (30 µg) transfected with SARS-CoV NP
    Lane 3 : HEK-293T whole cell extracts (30 µg) transfected with SARS-CoV-2 (COVID-19) NP
    Lane 4 : HEK-293T whole cell extracts (30 µg) transfected with MERS-CoV NP

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody


    Upper panel probed with ab281298.

  • Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL453] - BSA and Azide free (ab281298)
    Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL453] - BSA and Azide free (ab281298)

    Mock and SARS-CoV-2 nucleocapsid transfected HEK-293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 nucleocapsid protein (green) was labeled using ab281298 at 1/1000 dilution immunocytochemical analysis. Fluoroshield with DAPI (blue).

  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL453] - BSA and Azide free (ab281298)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL453] - BSA and Azide free (ab281298)
    All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL453] - BSA and Azide free (ab281298) at 1/5000 dilution

    Lane 1 : HEK-293T whole cell extracts (30 µg) non-transfected
    Lane 2 : HEK-293T whole cell extracts (30 µg) transfected with SARS-CoV-2 nucleocapsid

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody
  • Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL453] - BSA and Azide free (ab281298)
    Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL453] - BSA and Azide free (ab281298)
    All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL453] - BSA and Azide free (ab281298) at 1/5000 dilution

    Lane 1 : Vero E6 whole cell extracts (30 µg) non-transfected
    Lane 2 : Vero E6 whole cell extracts (30 µg) transfected with SARS-CoV-2 nucleocapsid

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody
  • Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL453] - BSA and Azide free (ab281298)
    Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL453] - BSA and Azide free (ab281298)

    Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (15.63-1000 ng/mL). Coated protein probed with ab281298 at 1 µg/mL. HRP-Rabbit IgG antibody at 1/10000 dilution used as detection antibody.

  • Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL453] - BSA and Azide free (ab281298)
    Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL453] - BSA and Azide free (ab281298)

    Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (50 ng). Coated protein probed with ab281298 (4.8*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (1:10000) detected bound primary antibody.
    EC50 : 0.006 nM

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

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