重组Anti-SARS-CoV-2 nucleocapsid蛋白抗体[HL146] - BSA and Azide free (ab281294)
![Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294)](https://www.abcam.cn/ps/products/281/ab281294/Images/ab281294-456632-SARSCoV2-nucleocapsid-protein-antibody-HL146-wb.jpg "Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [HL146] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free
- Suitable for: ELISA, WB, ICC, Sandwich ELISA, Indirect ELISA
概述
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产品名称
Anti-SARS-CoV-2 nucleocapsid蛋白抗体[HL146] - BSA and Azide free
参阅全部 SARS-CoV-2 nucleocapsid protein 一抗 -
描述
兔单克隆抗体[HL146] to SARS-CoV-2 nucleocapsid蛋白- BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: ELISA, WB, ICC, Sandwich ELISA, Indirect ELISAmore details -
种属反应性
与反应: SARS-CoV-2 -
免疫原
Recombinant full length protein corresponding to Human coronavirus SARS-CoV-2 nucleocapsid protein. Strain Wuhan-Hu-1
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阳性对照
- ICC: SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T cells. WB: SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T cells. ELISA: Recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.4
Constituent: 100% PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
HL146 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab281294于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
|
| WB |
1/1000 - 1/10000.
|
|
| ICC |
1/100 - 1/1000.
|
|
| Sandwich ELISA |
Use a concentration of 5 µg/ml.
|
|
| Indirect ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
WB
1/1000 - 1/10000. |
|
ICC
1/100 - 1/1000. |
|
Sandwich ELISA
Use a concentration of 5 µg/ml. |
|
Indirect ELISA
Use at an assay dependent concentration. |
靶标
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相关性
Nucleocapsid protein is a most abundant protein of coronavirus on the helical nucleocapsid of coronaviruses. N protein of SARS CoV-2 ab273530 is a structural protein required for RNA synthesis, and has RNA chaperone activity that may be involved in template switch. N protein enters the host cell with the viral RNA to facilitate its replication and process the virus particle assembly and release. N protein is a highly immunogenic phosphoprotein also implicated in modulating cell signalling pathways. Coronavirus nucleocapsid proteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. -
别名
- 2019-nCoV nucleocapsid protein antibody
- N antibody
- NC antibody
see all
图片
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Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294)All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294) at 1/5000 dilution
Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : SARS-CoV NP transfected 293T whole cell extracts
Lane 3 : SARS-CoV-2 (COVID-19) NP transfected 293T whole cell extracts
Lane 4 : MERS-CoV NP transfected 293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody -
![Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294)](https://www.abcam.cn/ps/products/281/ab281294/Images/ab281294-4-SARSCoV2-covid19-nucleocapsid-antibody-HL146-immunocytomechemistry-hek293t.jpg)
Immunocytochemistry - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294)Mock and SARS-CoV-2 (COVID-19) nucleocapsid transfected 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) nucleocapsid was stained by ab281294 (green) at 1/1000 dilution by immunofluorescent analysis. Fluoroshield with DAPI (blue).
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![Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294)](https://www.abcam.cn/ps/products/281/ab281294/Images/ab281294-3-SARSCoV2-covid19-nucleocapsid-antibody-HL146-indirectelisa.jpg)
Indirect ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294)Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein at 50ng. Coated protein probed with ab281294 at 2.4*10-6 ~ 20 nM. HRP-conjugated Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody. EC50: 0.05 nM
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![Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294)](https://www.abcam.cn/ps/products/281/ab281294/Images/ab281294-2-SARSCoV2-covid19-nucleocapsid-antibody-HL146-westernblot-hek93T-cells.jpg)
Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294)All lanes : Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294) at 1/5000 dilution
Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : SARS-CoV-2 (COVID-19) NP transfected 293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody -
![Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294)](https://www.abcam.cn/ps/products/281/ab281294/Images/ab281294-1-SARSCoV2-covid19-nucleocapsid-antibody-HL146-sandwichelisa.jpg)
Sandwich ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [HL146] - BSA and Azide free (ab281294)Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein using ab281294 as capture antibody at concentration of 5 µg/ml and HRP-conjugated ab281299 as detection antibody at concentration of 1 µg/ml.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab281294 尚未被引用在任何文献中。
