重组Anti-SARS-CoV-2 nucleocapsid蛋白抗体[1A6] -人IgG1 (Chimeric) (ab272852)
![Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Human IgG1 (Chimeric) (ab272852)](https://www.abcam.cn/ps/products/272/ab272852/Images/ab272852-389948-Mouse-monoclonal-1A6-to-SARSCoV2-nucleocapsid-protein-western-blot.jpg "Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Human IgG1 (Chimeric) (ab272852)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Human monoclonal [1A6] to SARS-CoV-2 nucleocapsid protein
- Suitable for: WB, ELISA
Related conjugates and formulations
概述
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产品名称
Anti-SARS-CoV-2 nucleocapsid蛋白抗体[1A6] -人IgG1 (Chimeric)
参阅全部 SARS-CoV-2 nucleocapsid protein 一抗 -
描述
人单克隆抗体[1A6] to SARS-CoV-2 nucleocapsid蛋白 -
宿主
Human -
经测试应用
适用于: WB, ELISAmore details -
种属反应性
与反应: SARS-CoV-2 -
免疫原
Recombinant full length protein corresponding to SARS-CoV-2 nucleocapsid protein aa 1-419. Recombinant anti-SARS-CoV-2 Nucleoprotein Mouse ScFv is expressed from 293 cells (HEK293) with a human IgG1 Fc tag on C-terminal.
Database link: P0DTC9 -
阳性对照
- ELISA: Human Novel Coronavirus (SARS-CoV-2/ 2020-nCoV) samples. WB: His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E.coli.
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常规说明
Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Human IgG1 (Chimeric) (ab272852) is a recombinant human/mouse chimeric monoclonal antibody consisting of Mouse scFv fused with human IgG1.
As the Fc region is human, an anti-Human secondary antibody should be used for detection.
Applications overview
Tick: Tested and Guaranteed to work X: Will not work —: No data
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Screened the positive clones from mouse ScFv antibody libraries, then expressed in 293F cells. -
克隆
单克隆 -
克隆编号
1A6 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272852于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/5000.
|
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| ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
1/500 - 1/5000. |
|
ELISA
Use at an assay dependent concentration. |
靶标
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相关性
Nucleocapsid protein is a most abundant protein of coronavirus on the helical nucleocapsid of coronaviruses. N protein of SARS CoV-2 ab273530 is a structural protein required for RNA synthesis, and has RNA chaperone activity that may be involved in template switch. N protein enters the host cell with the viral RNA to facilitate its replication and process the virus particle assembly and release. N protein is a highly immunogenic phosphoprotein also implicated in modulating cell signalling pathways. Coronavirus nucleocapsid proteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. -
别名
- 2019-nCoV nucleocapsid protein antibody
- N antibody
- NC antibody
see all
图片
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Western blot - Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Human IgG1 (Chimeric) (ab272852)Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Human IgG1 (Chimeric) (ab272852) at 1/1000 dilution + His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E.coli, 200 ng
Secondary
Peroxidase Goat Anti-Human IgG, Fc? Fragment Specific at 1/20000 dilution -
![ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Human IgG1 (Chimeric) (ab272852)](https://www.abcam.cn/ps/products/272/ab272852/Images/ab272852-390095-Anti-sars-cov2-nucleocapsid-protein-antibody-1a6-coronavirus-elisa.jpg)
ELISA - Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Human IgG1 (Chimeric) (ab272852)Binding Activity of ab272852 with SARS-CoV-2 nucleocapsid protein.Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 nucleocapsid protein at 5 μg/ml can bind ab272852. The EC50 of ab272852 is 43.50-118.4 ng/ml. -
![Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Human IgG1 (Chimeric) (ab272852)](https://www.abcam.cn/ps/products/272/ab272852/Images/ab272852-5-benefits-of-recombinant-antibodies.png)
Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Human IgG1 (Chimeric) (ab272852)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab272852 被引用在 6 文献中.
- Khan WH et al. Dimerization of SARS-CoV-2 nucleocapsid protein affects sensitivity of ELISA based diagnostics of COVID-19. Int J Biol Macromol 200:428-437 (2022). PubMed: 35051498
- Su Y et al. Multiple early factors anticipate post-acute COVID-19 sequelae. Cell 185:881-895.e20 (2022). PubMed: 35216672
- Schmidt N et al. The SARS-CoV-2 RNA-protein interactome in infected human cells. Nat Microbiol 6:339-353 (2021). PubMed: 33349665
- Klausberger M et al. A comprehensive antigen production and characterisation study for easy-to-implement, specific and quantitative SARS-CoV-2 serotests. EBioMedicine 67:103348 (2021). PubMed: 33906067
- Lutomski CA et al. Multiple Roles of SARS-CoV-2 N Protein Facilitated by Proteoform-Specific Interactions with RNA, Host Proteins, and Convalescent Antibodies. JACS Au 1:1147-1157 (2021). PubMed: 34462738
- Allison G et al. A Fabry-Pérot cavity coupled surface plasmon photodiode for electrical biomolecular sensing. Nat Commun 12:6483 (2021). PubMed: 34759292