Anti-SARS-CoV-2 envelope蛋白抗体- Coronavirus (ab272503)
Key features and details
- Rabbit polyclonal to SARS-CoV-2 envelope protein - Coronavirus
- Suitable for: ELISA, IHC-P
- Isotype: IgG
概述
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产品名称
Anti-SARS-CoV-2 envelope蛋白抗体- Coronavirus
参阅全部 SARS-CoV-2 envelope protein 一抗 -
描述
兔多克隆抗体to SARS-CoV-2 envelope蛋白- Coronavirus -
宿主
Rabbit -
经测试应用
适用于: ELISA, IHC-Pmore details -
种属反应性
与反应: SARS-CoV-2 -
免疫原
Synthetic peptide within Human coronavirus SARS-CoV-2 envelope protein aa 1-100 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. 10 amino acid peptide
Database link: QHD43418.1
(Peptide available asab273064) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272503于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use a concentration of 1 µg/ml.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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ELISA
Use a concentration of 1 µg/ml. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 envelope protein antibody - Coronavirus (ab272503)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of COVID-19 patient lung tissue labeling SARS-CoV-2 envelope protein with ab272503 at 1 µg/mL. Tissue was blocked with 10% serum for 1 hour at room temperature. Heat mediated antigen retrieval was performed using citrate buffer pH 6. Samples were incubated with the primary antibody overnight at 4°C. An HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/250). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Strong positive signal observed in macrophage cells of COVID-19 patient lung but not in non-COVID-19 patient lung.
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ELISA - Anti-SARS-CoV-2 envelope protein antibody - Coronavirus (ab272503)A direct ELISA was performed using antigen or control peptide as coating antigen and ab272503 as the capture antibody at 1 μg/ml, followed by the secondary antibody goat anti-rabbit IgG HRP conjugate at 1/20000 dilution. Detection range was from 32ng/ml to 2000 ng/ml.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272503 尚未被引用在任何文献中。