Sarcomeric Alpha Actinin重组抗体[EPR28776-83]_Sarcomeric Alpha Actinin抗体(ab317695)

产品名称

Anti-Sarcomeric Alpha Actinin抗体[EPR28776-83]
参阅全部 Sarcomeric Alpha Actinin 一抗
描述

兔单克隆抗体[EPR28776-83] to Sarcomeric Alpha Actinin
宿主

Rabbit
特异性

Unsuitable for human IHC.
经测试应用

适用于: ICC/IF, Dot blot, IHC-P, WBmore details
不适用于: Flow Cyt (Intra) or IP
种属反应性

与反应: Mouse, Rat, Human
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: Human heart, Human skeletal muscle, C2C12 differentiation to muscle for 5 days whole, Mouse skeletal muscle, Mouse heart, Rat skeletal muscle and Rat heart lysates. IHC-P: Mouse skeletal muscle, Rat skeletal muscle and Rat cardiac muscle tissues. ICC/IF: RD and C2C12 cells. Dot: His-tagged human ACTN2 recombinant protein
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
存储溶液

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR28776-83
同种型

IgG
研究领域

Alternative Versions
- Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] - BSA and Azide free (ab317696)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Related Products
- Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
- Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

The Abpromise guarantee

Abpromise™承诺保证使用ab317695于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
ICC/IF		1/500.
Dot blot		1/1000.
IHC-P		1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB		1/1000. Predicted molecular weight: 103 kDa.

说明
ICC/IF 1/500.
Dot blot 1/1000.
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 103 kDa.

应用说明

Is unsuitable for Flow Cyt (Intra) or IP.

功能

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
组织特异性

Expressed in both skeletal and cardiac muscle.
疾病相关

Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
序列相似性

Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats.
细胞定位

Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.
Target information above from: UniProt accession P35609 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 88 Human
- Entrez Gene: 11472 Mouse
- Entrez Gene: 291245 Rat
- Omim: 102573 Human
- SwissProt: P35609 Human
- SwissProt: Q9JI91 Mouse
- Unigene: 498178 Human
- Unigene: 37638 Mouse
别名
- Actin binding protein antibody
- Actinin alpha 2 antibody
- ACTN 2 antibody
- ACTN2 antibody
- ACTN2_HUMAN antibody
- Alpha actinin 2 antibody
- Alpha actinin skeletal muscle antibody
- Alpha actinin skeletal muscle isoform 2 antibody
- Alpha-actinin skeletal muscle isoform 2 antibody
- Alpha-actinin-2 antibody
- CMD1AA antibody
- F actin cross linking protein antibody
- F-actin cross-linking protein antibody
see all

Western blot - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] (ab317695)

All lanes : Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] (ab317695) at 1/1000 dilution

Lane 1 : Human heart tissue lysate at 20 µg
Lane 2 : Human skeletal muscle tissue lysate at 50 µg
Lane 3 : Human liver tissue lysate at 50 µg
Lane 4 : Human pancreas tissue lysate at 50 µg
Lane 5 : Human placenta tissue lysate at 50 µg

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 103 kDa
Observed band size: 103 kDa

Exposure time: 180 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Negative control: liver, pancreas, placenta (PMID:11440986).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Western blot - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] (ab317695)

All lanes : Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] (ab317695) at 1/1000 dilution

Lane 1 : Untreated C2C12 (mouse myoblast) whole cell lysate
Lane 2 : C2C12 differentiation to muscle for 5 days whole cell lysate
Lane 3 : Mouse skeletal muscle tissue lysate
Lane 4 : Mouse heart tissue lysate
Lane 5 : Mouse liver tissue lysate
Lane 6 : Mouse placenta tissue lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 103 kDa
Observed band size: 103 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Negative control: liver, placenta (PMID:11440986).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time: Lanes 1-2: 180 seconds, Lanes 3-6: 26 seconds
Western blot - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] (ab317695)

All lanes : Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] (ab317695) at 1/1000 dilution

Lane 1 : Rat skeletal muscle tissue lysate
Lane 2 : Rat heart tissue lysate
Lane 3 : Rat liver tissue lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 103 kDa
Observed band size: 103 kDa

Exposure time: 26 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Negative control: liver (PMID:11440986).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] (ab317695)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Sarcomeric Alpha Actinin with ab317695 at 1/100 (5.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
Positive staining on mouse skeletal muscle. The section was incubated with ab317695 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND ® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] (ab317695)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Sarcomeric Alpha Actinin with ab317695 at 1/100 (5.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
Positive staining on rat skeletal muscle. The section was incubated with ab317695 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND ® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] (ab317695)

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling Sarcomeric Alpha Actinin with ab317695 at 1/100 (5.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
Positive staining on rat cardiac muscle. The section was incubated with ab317695 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND ® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] (ab317695)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Sarcomeric Alpha Actinin with ab317695 at 1/100 (5.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
Negative control: no staining on mouse liver. The section was incubated with ab317695 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND ® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] (ab317695)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Sarcomeric Alpha Actinin with ab317695 at 1/100 (5.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
Negative control: no staining on rat liver. The section was incubated with ab317695 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND ® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] (ab317695)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RD (human muscle multinucleated spindle-shaped cell) cells labelling Sarcomeric Alpha Actinin with ab317695 at 1/500 (1.05 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor ® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing cytoplasmic and membranous staining in RD cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor ® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor ® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] (ab317695)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C2C12 (mouse myoblast) cells labelling Sarcomeric Alpha Actinin with ab317695 at 1/500 (1.05 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor ® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing cytoplasmic and membranous staining in differentiated C2C12 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor ® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor ® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Dot Blot - Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] (ab317695)

Dot blot analysis of Sarcomeric Alpha Actinin using ab317695 at 1:1000 (0.525 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.

Lane 1: His-tagged human ACTN2 recombinant protein
Lane 2: His-tagged human ACTN4 recombinant protein
Lane 3: His-tagged human ACTN1 recombinant protein
Lane 4: His-tagged human ACTN3 recombinant protein

Exposure time: 180 seconds.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

This antibody does not cross-react with human ACTN1, human ACTN3 and human ACTN4.

Anti-His tag antibody (1:10000) as total protein control.
Anti-Sarcomeric Alpha Actinin antibody [EPR28776-83] (ab317695)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

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概述

产品名称

描述

宿主

特异性

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Related Products

应用

The Abpromise guarantee

靶标

功能

组织特异性

疾病相关

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

Certificate of Compliance

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