重组Anti-Sarcomeric Alpha Actinin抗体[EP2530Y] (ab68168)
![Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2530Y] (ab68168)](https://www.abcam.cn/ps/products/68/ab68168/Images/ab68168-82379-anti-sarcomeric-alpha-actinin-antibody-ep2530y-western-blot.gif "Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2530Y] (ab68168)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2530Y] to Sarcomeric Alpha Actinin
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Sarcomeric Alpha Actinin抗体[EP2530Y]
参阅全部 Sarcomeric Alpha Actinin 一抗 -
描述
兔单克隆抗体[EP2530Y] to Sarcomeric Alpha Actinin -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human Sarcomeric Alpha Actinin aa 750-850 (C terminal). The exact sequence is proprietary.
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阳性对照
- Human skeletal muscle lysate and human muscle tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EP2530Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab68168于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| IHC-P |
| 说明 |
|---|
应用说明
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP: 1/40.
WB: 1/20000 - 1/50000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
Is unsuitable for Flow Cyt or ICC/IF.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IP: 1/40.
WB: 1/20000 - 1/50000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
Is unsuitable for Flow Cyt or ICC/IF.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. -
组织特异性
Expressed in both skeletal and cardiac muscle. -
疾病相关
Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
序列相似性
Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats. -
细胞定位
Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle. - Information by UniProt
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数据库链接
- Entrez Gene: 88 Human
- Entrez Gene: 11472 Mouse
- Entrez Gene: 291245 Rat
- Omim: 102573 Human
- SwissProt: P35609 Human
- SwissProt: Q9JI91 Mouse
- Unigene: 498178 Human
- Unigene: 37638 Mouse
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别名
- Actin binding protein antibody
- Actinin alpha 2 antibody
- ACTN 2 antibody
see all
图片
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Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2530Y] (ab68168)Anti-Sarcomeric Alpha Actinin antibody [EP2530Y] (ab68168) at 1/50000 dilution + human skeletal muscle lysate at 10 µg
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2530Y] (ab68168)](https://www.abcam.cn/ps/products/68/ab68168/Images/ab68168-82378-anti-sarcomeric-alpha-actinin-antibody-ep2530y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2530Y] (ab68168)ab68168 at 1/100 dilution staining Sarcomeric Alpha Actinin in human muscles by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Sarcomeric Alpha Actinin antibody [EP2530Y] (ab68168)](https://www.abcam.cn/ps/products/68/ab68168/Images/ab68168-3-benefits-of-recombinant-antibodies.png)
Anti-Sarcomeric Alpha Actinin antibody [EP2530Y] (ab68168)
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab68168 被引用在 3 文献中.
- Gan L et al. A cell-penetrating peptide enhances delivery and efficacy of phosphorodiamidate morpholino oligomers in mdx mice. Mol Ther Nucleic Acids 30:17-27 (2022). PubMed: 36189424
- Xu J et al. Optimization of Timing and Times for Administration of Atorvastatin-Pretreated Mesenchymal Stem Cells in a Preclinical Model of Acute Myocardial Infarction. Stem Cells Transl Med 8:1068-1083 (2019). PubMed: 31245934
- Papadimitriou ID et al. A "human knockout" model to investigate the influence of the a-actinin-3 protein on exercise-induced mitochondrial adaptations. Sci Rep 9:12688 (2019). PubMed: 31481717