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AB137346

Anti-Sarcomeric Alpha Actinin 抗体

Anti-Sarcomeric Alpha Actinin antibody

5

(8 Reviews)

|

(65 Publications)

Anti-Sarcomeric Alpha Actinin antibody (ab137346) is a rabbit polyclonal antibody detecting Sarcomeric Alpha Actinin in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Over 40 publications

查看别名

Alpha-actinin-2, Alpha-actinin skeletal muscle isoform 2, F-actin cross-linking protein, ACTN2

10 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody (AB137346)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody (AB137346)

Immunohistochemical analysis of paraffin-embedded mouse heart tissue labelling Sarcomeric Alpha Actinin with ab137346 at 1/2000. Antigen retrieval : Citrate buffer, pH 6.0, 15 minutes.

Immunocytochemistry/ Immunofluorescence - Anti-Sarcomeric Alpha Actinin antibody (AB137346)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Sarcomeric Alpha Actinin antibody (AB137346)

Immunofluorescent analysis of ice-cold methanol fixed (5 minutes) HeLa cells labeling Sarcomeric Alpha Actinin with ab137346 at 1/500 dilution (4°C overnight), followed by Rabbit IgG antibody (Alexa Fluor 488) (keep from light) at 1/2000 dilution (room temperature for 1 hour) (green). Blue : Hoechst 33342 staining.

Blocking : 2.5% BSA/PBS for 30 minutes.

Washing : PBS, 3 x 3 minutes.

Immunocytochemistry/ Immunofluorescence - Anti-Sarcomeric Alpha Actinin antibody (AB137346)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Sarcomeric Alpha Actinin antibody (AB137346)

Immunofluorescence analysis of HeLa cells fixed in ice-cold MeOH for 5 min labelling alpha Actinin 2 protein at cytoskeleton using ab137346 at a 1/500 dilution (Green). DAPI was used for nuclear staining (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-Sarcomeric Alpha Actinin antibody (AB137346)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Sarcomeric Alpha Actinin antibody (AB137346)

Immunofluorescence analysis of HeLa cells fixed in ice-cold MeOH for 5 min labelling alpha Actinin 2 protein at cytoskeleton using ab137346 at a 1/500 dilution (Green). DAPI was used for nuclear staining (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody (AB137346)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody (AB137346)

Immunohistochemical analysis of paraffin-embedded mouse muscle tissue stained for alpha Actinin 2 protein at cytoplasm with ab137346 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody (AB137346)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody (AB137346)

Immunohistochemical analysis of paraffin-embedded mouse muscle tissue stained for alpha Actinin 2 protein at cytoplasm with ab137346 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody (AB137346)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody (AB137346)

Immunohistochemical analysis of paraffin-embedded rat muscle tissue stained for alpha Actinin 2 protein at cytoplasm in rat muscle with ab137346 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody (AB137346)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody (AB137346)

Immunohistochemical analysis of paraffin-embedded mouse muscle tissue stained for alpha Actinin 2 protein at cytoplasm with ab137346 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody (AB137346)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody (AB137346)

Immunohistochemical analysis of paraffin-embedded rat heart tissue labelling Sarcomeric Alpha Actinin with ab137346 at 1/2000. Antigen retrieval : Citrate buffer, pH 6.0, 15 minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody (AB137346)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody (AB137346)

Immunohistochemical analysis of paraffin-embedded mouse heart tissue stained for alpha Actinin 2 protein at cytoplasm with ab137346 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human, Zebrafish

应用

ICC/IF, IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Human ACTN2. The exact immunogen used to generate this antibody is proprietary information.

P35609

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替代产品

Primary Antibodies

AB68167

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (AB68167)

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] - BSA and Azide free (AB220817)

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Primary Antibodies

AB113086

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody (AB113086)

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Primary Antibodies

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Primary Antibodies

AB68168

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搭配使用产品

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Primary Antibodies

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Primary Antibodies

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p>Use ice-cold Methanol as fixation.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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产品详情

What is this antibody validated in?
Anti-Sarcomeric Alpha Actinin antibody (ab137346) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of Sarcomeric Alpha Actinin?
Anti-Sarcomeric Alpha Actinin (ab137346) specifically detects a band for Sarcomeric Alpha Actinin (UniProt: P35609) at a molecular weight of 103kDa.

Trusted by the scientific community
Anti-Sarcomeric Alpha Actinin (ab137346) was first used in a scientific publication in 2012 and has been cited over 40 times in peer-reviewed journals.

Reviewed by scientists
Anti-Sarcomeric Alpha Actinin (ab137346) has over 5 independent reviews from customers.
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Sarcomeric Alpha Actinin often referred to as alpha-actinin or A-actinin is a protein that functions mechanically as a cross-linker of actin filaments in the sarcomere. It plays a critical role in organizing the actin cytoskeleton providing structural integrity and facilitating muscle contraction. The protein typically has a molecular mass of approximately 100 kDa. It is expressed in a variety of tissues including skeletal and cardiac muscle where it establishes the architecture of the Z-disc by anchoring actin filaments.
Biological function summary

Sarcomeric alpha actinin associates with multiple proteins to form a complex that coordinates muscle function. It not only serves as a structural component but also participates in signaling processes influencing muscle repair and growth. Alpha actinin connects to other cytoskeletal proteins and adaptors coordinating with integrin pathways to transmit external mechanical signals into cellular responses. The ability of alpha actinin to interact with a range of proteins like filamin and spectrin enhances its role in maintaining cellular architecture.

Pathways

Alpha actinin is critical in both the integrin and dystrophin-glycoprotein complex pathways facilitating signal transduction and maintaining muscle stability. Its interaction with actin filaments is important for pathways involving cell adhesion and motility. Within these pathways actin-related proteins such as ACTN2 and titin partner with alpha actinin to sustain cellular structure and function during dynamic processes including muscle contraction and relaxation.

Mutations or defects in alpha actinin have implications in muscular dystrophy and cardiomyopathy. These conditions often involve disruptions in the muscle's structural framework and mechanical signaling where alpha actinin's interaction with dystrophin becomes significant. Additionally such disorders may showcase altered connectivity between alpha actinin and spectrin further affecting muscle integrity and function in pathological conditions.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
See full target information ACTN2
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文献 (65)

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