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AB326352

Anti-SAP97 antibody [G28]

Anti-SAP97 antibody [G28]

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Rabbit Recombinant Monoclonal SAP97 antibody. Suitable for WB, ICC/IF and reacts with Rat, Human, Mouse samples.

查看别名

Dlgh1, Dlg1, Disks large homolog 1, Synapse-associated protein 97, SAP-97, SAP97

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody [G28] (AB326352)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody [G28] (AB326352)

Immunocytochemistry/Immunofluorescence analysis. Tissue : Neuroblastoma cells (Kelly). Species : Human. Fixation : 4% PFA for 10 min at RT. Permeabilization : 0.15% TritonX for 15 min at RT. Blocking : 10% goat serum for 40 min at RT. Primary Antibody : ab326352 at 1 : 100 for 1 hour at RT. Secondary Antibody : Goat anti-Rabbit IgG-AlexaFluor488 (green) at 1 : 1000 for 1 hour at RT in the dark. Counterstain : DAPI (blue) nuclear stain at 1 : 1000 for 5 min at RT in the dark.

Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody [G28] (AB326352)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody [G28] (AB326352)

Immunocytochemistry/Immunofluorescence analysis. Tissue : Pheochromocytoma cells (PC-12). Species : Rat. Fixation : 4% PFA for 10 min at RT. Permeabilization : 0.15% TritonX for 15 min at RT. Blocking : 10% goat serum for 40 min at RT. Primary Antibody : ab326352 at 1 : 100 for 1 hour at RT. Secondary Antibody : Goat anti-Rabbit IgG-AlexaFluor488 (green) at 1 : 1000 for 1 hour at RT in the dark. Counterstain : DAPI (blue) nuclear stain at 1 : 1000 for 5 min at RT in the dark.

Western blot - Anti-SAP97 antibody [G28] (AB326352)
  • WB

Supplier Data

Western blot - Anti-SAP97 antibody [G28] (AB326352)

Western blot analysis showing detection of Human SAP97 protein in HeLa whole cell lysate. Block : 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody : ab326352 at 1 : 1000 for 2 hours at RT. Secondary Antibody : Goat anti-rabbit IgG-HRP (H&L) at 1 : 5000 for 1 hour at RT. Color Development : Chemiluminescent for HRP for 1 min at RT in the dark. Exposed 5 seconds.

All lanes:

Western blot - Anti-SAP97 antibody [G28] (ab326352) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG-HRP (H&L) at 1/5000 dilution

false

Exposure time: 5s

Western blot - Anti-SAP97 antibody [G28] (AB326352)
  • WB

Supplier Data

Western blot - Anti-SAP97 antibody [G28] (AB326352)

Western blot analysis showing detection of Rat SAP97 protein in a Rat brain membrane lysate. Block : 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody : ab326352 at 1 : 1000 for 2 hours at RT. Secondary Antibody : Goat anti-rabbit IgG-HRP (H&L) at 1 : 5000 for 1 hour at RT. Color Development : Chemiluminescent for HRP for 3 min at RT in the dark. Exposed 6.54 seconds.

All lanes:

Western blot - Anti-SAP97 antibody [G28] (ab326352) at 1/1000 dilution

All lanes:

Rat brain membrane lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit IgG-HRP (H&L) at 1/5000 dilution

false

Exposure time: 6.54s

Western blot - Anti-SAP97 antibody [G28] (AB326352)
  • WB

Supplier Data

Western blot - Anti-SAP97 antibody [G28] (AB326352)

Western blot analysis showing detection of Mouse SAP97 protein in Mouse Brain lysate. Block : 5% BSA for 1 hour at RT. Primary Antibody : ab326352 at 1 : 1000 for 2 hours at RT. Secondary Antibody : Goat anti-rabbit IgG-HRP (H&L) at 1 : 5000 for 1 hour at RT. Color Development : Chemiluminescent for HRP for 3 min at RT in the dark. Exposed 1 second.

All lanes:

Western blot - Anti-SAP97 antibody [G28] (ab326352) at 1/1000 dilution

All lanes:

Mouse Brain lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG-HRP (H&L) at 1/5000 dilution

false

Exposure time: 1s

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

G28

亚型

IgG

不含载体蛋白

No

反应种属

Rat, Human, Mouse

应用

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Essential multidomain scaffolding protein required for normal development (By similarity). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. Promotes epithelial cell layer barrier function via maintaining cell-cell adhesion (By similarity). May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel (By similarity). During long-term depression in hippocampal neurons, it recruits ADAM10 to the plasma membrane (By similarity).
See full target information Dlg1

其他靶点

DLG1_HUMAN,
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

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