Anti-SAP30抗体(ab125187)
Key features and details
- Rabbit polyclonal to SAP30
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-SAP30抗体
参阅全部 SAP30 一抗 -
描述
兔多克隆抗体to SAP30 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rabbit, Horse, Dog, Pig, Chimpanzee, Gorilla, Chinese hamster, Orangutan, Elephant
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免疫原
Synthetic peptide, corresponding to a region within amino acids 170-220 of Human SAP30 (NP_003855.1).
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阳性对照
- Hela, Jurkat, NIH 3T3 and 293T whole cell lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125187于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 23 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 23 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Involved in the functional recruitment of the Sin3-histone deacetylase complex (HDAC) to a specific subset of N-CoR corepressor complexes. Capable of transcription repression by N-CoR. Active in deacetylating core histone octamers (when in a complex) but inactive in deacetylating nucleosomal histones. -
组织特异性
Expressed in all tissues tested with highest levels in pancreas, ovary, PBL, spleen and thymus; lowest levels in brain, placenta, lung and kidney. -
序列相似性
Belongs to the SAP30 family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 8819 Human
- Entrez Gene: 60406 Mouse
- Omim: 603378 Human
- SwissProt: O75446 Human
- SwissProt: O88574 Mouse
- Unigene: 591715 Human
- Unigene: 118 Mouse
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别名
- 30 kDa Sin3-associated polypeptide antibody
- Histone deacetylase complex subunit SAP 30 antibody
- Histone deacetylase complex subunit SAP30 antibody
see all
图片
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Western blot - Anti-SAP30 antibody (ab125187)All lanes : Anti-SAP30 antibody (ab125187) at 0.4 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 23 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-SAP30 antibody (ab125187)ab125187, at 6 µg/mg lysate, detecting SAP30 in HeLa cell lysate (1 mg of immunoprecipitate, 20% loaded in the lane) by Immunoprecipitation.
For the subsequent Western blot, ab125187 was used at 1 µg/ml.
The blot was developed using the ECL technique, with an exposure time of 10 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab125187 被引用在 3 文献中.
- Sato M et al. The lncRNA Caren antagonizes heart failure by inactivating DNA damage response and activating mitochondrial biogenesis. Nat Commun 12:2529 (2021). PubMed: 33953175
- Banks CAS et al. Differential HDAC1/2 network analysis reveals a role for prefoldin/CCT in HDAC1/2 complex assembly. Sci Rep 8:13712 (2018). PubMed: 30209338
- Banks CAS et al. A Structured Workflow for Mapping Human Sin3 Histone Deacetylase Complex Interactions Using Halo-MudPIT Affinity-Purification Mass Spectrometry. Mol Cell Proteomics 17:1432-1447 (2018). PubMed: 29599190