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重组RabMAb

重组Anti-SAP18抗体[EPR12956(B)] (ab175920)

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Western blot - Anti-SAP18 antibody [EPR12956(B)] (ab175920)
  • Immunocytochemistry/ Immunofluorescence - Anti-SAP18 antibody [EPR12956(B)] (ab175920)
  • Immunoprecipitation - Anti-SAP18 antibody [EPR12956(B)] (ab175920)
  • Anti-SAP18 antibody [EPR12956(B)] (ab175920)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12956(B)] to SAP18
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-SAP18抗体[EPR12956(B)]
    参阅全部 SAP18 一抗

  • 描述

    兔单克隆抗体[EPR12956(B)] to SAP18

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, ICC/IF, IPmore details
    不适用于: Flow Cyt or IHC-P

  • 种属反应性

    与反应: Rat, Human
    预测可用于: Mouse

  • 免疫原

    Synthetic peptide within Human SAP18 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: O00422

  • 阳性对照

    • Jurkat, K562, 293T and PC-12 cell lysates. 293T cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.2
  • Concentration information loading...

  • 纯度

    Tissue culture supernatant

  • 克隆

    单克隆

  • 克隆编号

    EPR12956(B)

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab175920于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
ICC/IF
1/10 - 1/100.
IP
1/10 - 1/100.
说明
WB
1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
ICC/IF
1/10 - 1/100.
IP
1/10 - 1/100.
应用说明
Is unsuitable for Flow Cyt or IHC-P.

靶标

  • 功能

    Component of the SIN3-repressing complex. Enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism.

  • 组织特异性

    Ubiquitous.

  • 序列相似性

    Belongs to the SAP18 family.

  • 细胞定位

    Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • 18 kDa Sin3-associated polypeptide antibody
    • 2HOR0202 antibody
    • Cell growth-inhibiting gene 38 protein antibody
    • Histone deacetylase complex subunit SAP18 antibody
    • SAP18 antibody
    • SAP18_HUMAN antibody
    • Sin3-associated polypeptide p18 antibody
    see all

图片

  • Western blot - Anti-SAP18 antibody [EPR12956(B)] (ab175920)
    Western blot - Anti-SAP18 antibody [EPR12956(B)] (ab175920)
    All lanes : Anti-SAP18 antibody [EPR12956(B)] (ab175920) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : Hela cell lysate
    Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate
    Lane 5 : PC-12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 18 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SAP18 antibody [EPR12956(B)] (ab175920)
    Immunocytochemistry/ Immunofluorescence - Anti-SAP18 antibody [EPR12956(B)] (ab175920)

    Immunofluorescent staining of 293T cells labeling SAP18 with ab175920 at 1/100 dilution.

  • Immunoprecipitation - Anti-SAP18 antibody [EPR12956(B)] (ab175920)
    Immunoprecipitation - Anti-SAP18 antibody [EPR12956(B)] (ab175920)

    Western blot analysis on immunoprecipitation pellet from K562 cell lysate labeling SAP18 with ab175920 at 1/10 dilution

  • Anti-SAP18 antibody [EPR12956(B)] (ab175920)
    Anti-SAP18 antibody [EPR12956(B)] (ab175920)

文献 (0)

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