重组Anti-SAMM50/SAM50抗体[EPR8718] (ab133709)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8718] to SAMM50/SAM50
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-SAMM50/SAM50抗体[EPR8718]
参阅全部 SAMM50/SAM50 一抗 -
描述
兔单克隆抗体[EPR8718] to SAMM50/SAM50 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC/IF or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human fetal heart lysate, A431 cell lysate, HeLa cell lysate, HepG2 cell lysate IHC-P: Human kidney tissue, Human ovarian carcinoma tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 1.30 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8718 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133709于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval before commencing with IHC staining protocol |
说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval before commencing with IHC staining protocol |
靶标
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功能
May be required for the assembly pathway of mitochondrial outer membrane proteins. -
序列相似性
Belongs to the SAM50/omp85 family. -
结构域
Its C-terminal part seems to contain many membrane-spanning sided beta-sheets, that have the potential to adopt a transmembrane beta-barrel type structure. -
细胞定位
Mitochondrion outer membrane. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 25813 Human
- Entrez Gene: 68653 Mouse
- Entrez Gene: 300111 Rat
- Omim: 612058 Human
- SwissProt: Q9Y512 Human
- SwissProt: Q8BGH2 Mouse
- SwissProt: Q6AXV4 Rat
- Unigene: 505824 Human
see all -
别名
- CGI 51 antibody
- CGI-51 antibody
- FLJ35825 antibody
see all
图片
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All lanes : Anti-SAMM50/SAM50 antibody [EPR8718] (ab133709) at 1/1000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 52 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMM50/SAM50 antibody [EPR8718] (ab133709)
ab133709, at 1/50 dilution, staining SAMM50/SAM50 in paraffin embedded Human kidney tissue using immunohistochemical analysis.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMM50/SAM50 antibody [EPR8718] (ab133709)
ab133709, at 1/50 dilution, staining SAMM50/SAM50 in paraffin embedded Human ovarian carcinoma tissue using immunohistochemical analysis.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab133709 被引用在 6 文献中.
- Xu R et al. Samm50 Promotes Hypertrophy by Regulating Pink1-Dependent Mitophagy Signaling in Neonatal Cardiomyocytes. Front Cardiovasc Med 8:748156 (2021). PubMed: 34631840
- Li Z et al. The role of SAMM50 in non-alcoholic fatty liver disease: from genetics to mechanisms. FEBS Open Bio 11:1893-1906 (2021). PubMed: 33728819
- Elouej S et al. Loss of MTX2 causes mandibuloacral dysplasia and links mitochondrial dysfunction to altered nuclear morphology. Nat Commun 11:4589 (2020). PubMed: 32917887
- Koyano F et al. Parkin-mediated ubiquitylation redistributes MITOL/March5 from mitochondria to peroxisomes. EMBO Rep 20:e47728 (2019). PubMed: 31602805
- Le Guerroué F et al. Autophagosomal Content Profiling Reveals an LC3C-Dependent Piecemeal Mitophagy Pathway. Mol Cell 68:786-796.e6 (2017). PubMed: 29149599
- Ding C et al. Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure. Sci Rep 5:16064 (2015). PubMed: 26530328