SAMHD1抗体[OTI1A1]| Abcam中文官网

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Western blot - Anti-SAMHD1 antibody [OTI1A1] (ab128107)

Key features and details

Mouse monoclonal [OTI1A1] to SAMHD1
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
Knockout validated
Reacts with: Mouse, Rat, Dog, Human, African green monkey
Isotype: IgG2b

产品名称

Anti-SAMHD1抗体[OTI1A1]
参阅全部 SAMHD1 一抗
描述

小鼠单克隆抗体[OTI1A1] to SAMHD1
宿主

Mouse
经测试应用

适用于: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
种属反应性

与反应: Mouse, Rat, Dog, Human, African green monkey
免疫原

Recombinant full length protein corresponding to Human SAMHD1 aa 1-626. Produced in HEK-293T cells (NP_056289).
Sequence:
MQRADSEQPSKRPRCDDSPRTPSNTPSAEADWSPGLELHPDYKTWGPEQV CSFLRRGGFEEPVLLKNIRENEITGALLPCLDESRFENLGVSSLGERKKL LSYIQRLVQIHVDTMKVINDPIHGHIELHPLLVRIIDTPQFQRLRYIKQL GGGYYVFPGASHNRFEHSLGVGYLAGCLVHALGEKQPELQISERDVLCVQ IAGLCHDLGHGPFSHMFDGRFIPLARPEVKWTHEQGSVMMFEHLINSNGI KPVMEQYGLIPEEDICFIKEQIVGPLESPVEDSLWPYKGRPENKSFLYEI VSNKRNGIDVDKWDYFARDCHHLGIQNNFDYKRFIKFARVCEVDNELRIC ARDKEVGNLYDMFHTRNSLHRRAYQHKVGNIIDTMITDAFLKADDYIEIT GAGGKKYRISTAIDDMEAYTKLTDNIFLEILYSTDPKLKDAREILKQIEY RNLFKYVGETQPTGQIKIKREDYESLPKEVASAKPKVLLDVKLKAEDFIV DVINMDYGMQEKNPIDHVSFYCKTAPNRAIRITKNQVSQLLPEKFAEQLI RVYCKKVDRKSLYAARQYFVQWCADRNFTKPQDGDVIAPLITPQKKEWND STSVQNPTRLREASKSRVQLFKDDPM

Database link: Q9Y3Z3
Run BLAST with Run BLAST with
阳性对照
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY SAMHD1 cDNA; HAP1, K562, HepG2, HeLa, SVT2, A549, COS-7, Jurkat, MDCK, PC-12, and MCF7 cell extracts; Mouse spleen extract. IHC-P: Human kidney, kidney carcinoma, liver, ovary adenocarcinoma, pancreas, thyroid carcinoma, prostate carcinoma, bladder carcinoma and tonsil tissues. ICC/IF: COS-7 cells transiently transfected with pCMV6-ENTRY SAMHD1. Flow Cyt (Intra): HEK-293T cells transfected with pCMV6-ENTRY SAMHD1; HeLa and Jurkat cells.
常规说明

The clone number has been updated from 1A1 to OTI1A1, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
存储溶液

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
纯度

Affinity purified
纯化说明

Purified from cell culture supernatant.
克隆

单克隆
克隆编号

OTI1A1
同种型

IgG2b
研究领域

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Isotype control
- Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
Positive Controls
Recombinant Protein
- Recombinant Human SAMHD1 protein (ab153254)
Related Products
- Recombinant Human SAMHD1 protein (ab153254)

The Abpromise guarantee

Abpromise™承诺保证使用ab128107于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB		1/500 - 1/2000. Predicted molecular weight: 72 kDa.
IHC-P		1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF		1/100.
Flow Cyt (Intra)		1/100.

说明
WB 1/500 - 1/2000. Predicted molecular weight: 72 kDa.
IHC-P 1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100.
Flow Cyt (Intra) 1/100.

功能

Putative nuclease involved in innate immune response by acting as a negative regulator of the cell-intrinsic antiviral response. May play a role in mediating proinflammatory responses to TNF-alpha signaling.
组织特异性

Expressed in heart, skeletal muscle, spleen, liver, small intestine, placenta, lung and peripheral blood leukocytes. No expression is seen in brain and thymus.
疾病相关

Defects in SAMHD1 are the cause of Aicardi-Goutieres syndrome type 5 (AGS5) [MIM:612952]. A form of Aicardi-Goutieres syndrome, a genetically heterogeneous disease characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection. Clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Severe neurological dysfunctions manifest in infancy as progressive microcephaly, spasticity, dystonic posturing and profound psychomotor retardation. Death often occurs in early childhood.
序列相似性

Belongs to the SAMHD1 family.
Contains 1 HD domain.
Contains 1 SAM (sterile alpha motif) domain.
细胞定位

Nucleus.
Target information above from: UniProt accession Q9Y3Z3 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 25939 Human
- Entrez Gene: 56045 Mouse
- Entrez Gene: 311580 Rat
- Omim: 606754 Human
- SwissProt: Q9Y3Z3 Human
- SwissProt: Q60710 Mouse
- Unigene: 580681 Human
- Unigene: 248478 Mouse
- Unigene: 468781 Mouse
see all
别名
- CHBL2 antibody
- DCIP antibody
- Dendritic cell derived IFNG induced protein antibody
- Dendritic cell-derived IFNG-induced protein antibody
- Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 antibody
- dNTPase antibody
- HD domain containing 1 antibody
- HDDC1 antibody
- Mg11 antibody
- Monocyte protein 5 antibody
- MOP 5 antibody
- MOP-5 antibody
- MOP5 antibody
- OTTHUMP00000030889 antibody
- SAM domain and HD domain 1 antibody
- SAM domain and HD domain containing protein 1 antibody
- SAM domain and HD domain-containing protein 1 antibody
- SAMH1_HUMAN antibody
- Samhd1 antibody
- SBBI88 antibody
see all

Western blot - Anti-SAMHD1 antibody [OTI1A1] (ab128107)

All lanes : Anti-SAMHD1 antibody [OTI1A1] (ab128107) at 1/500 dilution

Lane 1 : HEK-293 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Wild-type HAP1 cell lysate
Lane 4 : SAMHD1 knockout HAP1 cell lysate
Lane 5 : Human Spleen cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 72 kDa
Observed band size: 73,75 kDa why is the actual band size different from the predicted?

Western blot: Anti-SAMHD1 antibody [OTI1A1] (ab128107) staining at 1/500 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab128107 was shown to bind specifically to SAMHD1. A band was observed at 73/75 kDa in wild-type HEK-293 cell lysates with no signal observed at this size in SAMHD1 knockout cell line. To generate this image, wild-type and SAMHD1 knockout HEK-293 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.
Western blot - Anti-SAMHD1 antibody [OTI1A1] (ab128107)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: SAMHD1 knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab128107 observed at 70 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab128107 was shown to recognize SAMHD1 when SAMHD1 knockout samples were used, along with additional cross-reactive bands. Wild-type and SAMHD1 knockout samples were subjected to SDS-PAGE. ab128107 and ab181602 (loading control to GAPDH) were diluted 1/500 and 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-SAMHD1 antibody [OTI1A1] (ab128107)

All lanes : Anti-SAMHD1 antibody [OTI1A1] (ab128107) at 1/500 dilution

Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : SVT2 cell extract
Lane 4 : A549 (human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (Canine kidney cell line) cell extract
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (Human breast adenocarcinoma cell line) cell extract

Lysates/proteins at 35 µg per lane.

Predicted band size: 72 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (ab128107)

Paraffin-embedded human kidney tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (ab128107)

Paraffin-embedded human kidney carcinoma tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (ab128107)

Paraffin-embedded human liver tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (ab128107)

Paraffin-embedded human ovary adenocarcinoma tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (ab128107)

Paraffin-embedded human pancreas tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.
Western blot - Anti-SAMHD1 antibody [OTI1A1] (ab128107)

Anti-SAMHD1 antibody [OTI1A1] (ab128107) at 1/200 dilution + Mouse spleen extract at 10 µg

Predicted band size: 72 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (ab128107)

Paraffin-embedded human thyroid carcinoma tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (ab128107)

Paraffin-embedded human prostate carcinoma tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (ab128107)

Paraffin-embedded human bladder carcinoma tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMHD1 antibody [OTI1A1] (ab128107)

Paraffin-embedded human tonsil tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-SAMHD1 antibody [OTI1A1] (ab128107)

COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY SAMHD1 cDNA stained forSAMHD1 using ab128107 at 1/100 dilution in ICC/IF.
Flow Cytometry (Intracellular) - Anti-SAMHD1 antibody [OTI1A1] (ab128107)

Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY SAMHD1 overexpress plasmid (Red) or empty vector control plasmid (Blue) stained for SAMHD1 using ab128107 at 1/100 dilution.
Flow Cytometry (Intracellular) - Anti-SAMHD1 antibody [OTI1A1] (ab128107)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SAMHD1 using ab128107 at 1/100 dilution (Red) compared to a nonspecific negative control (Blue).
Flow Cytometry (Intracellular) - Anti-SAMHD1 antibody [OTI1A1] (ab128107)

Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells stained for SAMHD1 using ab128107 at 1/100 dilution (Red) compared to a nonspecific negative control (Blue).

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

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Datasheet download

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发表研究结果有使用 ab128107？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab128107 被引用在 9 文献中.

An S et al. SAMHD1-induced endosomal FAK signaling promotes human renal clear cell carcinoma metastasis by activating Rac1-mediated lamellipodia protrusion. Exp Mol Med 55:779-793 (2023). PubMed: 37009792
Tsai KD et al. Recombinant Klotho attenuates IFNγ receptor signaling and SAMHD1 expression through blocking NF-κB translocation in glomerular mesangial cells. Int J Med Sci 20:810-817 (2023). PubMed: 37213666
Liu J et al. De-ubiquitination of SAMHD1 by USP7 promotes DNA damage repair to overcome oncogenic stress and affect chemotherapy sensitivity. Oncogene 42:1843-1856 (2023). PubMed: 37081042
Wang T et al. SAMHD1 Mutations and Expression in Mantle Cell Lymphoma Patients. Front Oncol 11:763151 (2021). PubMed: 34976810
Rudd SG et al. Ribonucleotide reductase inhibitors suppress SAMHD1 ara-CTPase activity enhancing cytarabine efficacy. EMBO Mol Med 12:e10419 (2020). PubMed: 31950591
Tramentozzi E et al. The dNTP triphosphohydrolase activity of SAMHD1 persists during S-phase when the enzyme is phosphorylated at T592. Cell Cycle 17:1102-1114 (2018). PubMed: 30039733
Calonge E et al. Different Expression of Interferon-Stimulated Genes in Response to HIV-1 Infection in Dendritic Cells Based on Their Maturation State. J Virol 91:N/A (2017). PubMed: 28148784
Pilakka-Kanthikeel S et al. Sterile alpha motif and histidine/aspartic acid domain-containing protein 1 (SAMHD1)-facilitated HIV restriction in astrocytes is regulated by miRNA-181a. J Neuroinflammation 12:66 (2015). Flow Cyt . PubMed: 25890101
Franzolin E et al. The deoxynucleotide triphosphohydrolase SAMHD1 is a major regulator of DNA precursor pools in mammalian cells. Proc Natl Acad Sci U S A 110:14272-7 (2013). PubMed: 23858451

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Question

What is the concentration of lot GR82285?

Abcam community

Verified customer

Asked on Jun 04 2013

Answer

The concentration of lot GR82285 is 1.17mg/ml.

Abcam Scientific Support

回复于 Jun 04 2013

Question

for both Abs ab67192 and ab128107:
WB no bands at all
human cell line, PBMCs, etc.
also prepared nuclear extract
see band for GAPDH
Ab: 1/200 (recommended: 1/500 - 1/1000)
protein loaded: 1ug (recommended: 20-30 ug or more)

suggested: use more antibody, load more protein

Abcam community

Verified customer

Asked on Nov 07 2012

Answer

Thank you for contacting us.
I am sorry to hear that the antibodies don't seem to work as expected.

As we discussed over the phone, the following protocols tips can help with improving your results:

1) loading more protein onto the gel (e.g. 20-30 ug or more if needed)
2) using more primary antibody (e.g. 1/500 per recommended dilutions on the datasheet)
3) check if the secondary antibody is working well with other primary antibodies

I hope these tips will be of help.
Please do not hesitate to contact us if you need any more advice or information.

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Abcam Scientific Support

回复于 Nov 07 2012

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Key features and details

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

纯化说明

克隆

克隆编号

同种型

研究领域

相关产品

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

Related Products

应用

The Abpromise guarantee

靶标

功能

组织特异性

疾病相关

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (9)

客户评价及客户问答