Anti-SAMD9抗体(ab121663)
Key features and details
- Rabbit polyclonal to SAMD9
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SAMD9抗体
参阅全部 SAMD9 一抗 -
描述
兔多克隆抗体to SAMD9 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence: VSQKERRETS KQKQKGKENP DMANPSAMST TAKGSKSLKV ELIEDKIDYT KERQPSIDLT CVSYPFDEFS NPYRYKLDFS LQP, corresponding to amino acids 101-183 of Human SAMD9.
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阳性对照
- Human thyroid gland tissue; RT4 and U 251 MG cell lysates; A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121663于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/250 - 1/500.
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| IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| 说明 |
|---|
|
WB
1/250 - 1/500. |
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
靶标
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组织特异性
Widely expressed. Very low levels in skeletal muscle. Not detected in fetal brain. Down-regulated in aggressive fibromatosis, as well as in breast and colon cancers. -
疾病相关
Defects in SAMD9 are the cause of normophosphatemic familial tumoral calcinosis (NFTC) [MIM:610455]. NFTC is an uncommon life-threatening disorder characterized by massive periarticular, and seldom visceral, deposition of calcified tumors. -
序列相似性
Contains 1 SAM (sterile alpha motif) domain. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 54809 Human
- Omim: 610456 Human
- SwissProt: Q5K651 Human
- Unigene: 65641 Human
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别名
- C7orf5 antibody
- DRIF1 antibody
- expressed in aggressive fibromatosis antibody
see all
图片
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Western blot - Anti-SAMD9 antibody (ab121663)All lanes : Anti-SAMD9 antibody (ab121663) at 1/250 dilution
Lane 1 : RT4 cell lysates
Lane 2 : U251MG cell lysates
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysates
Lane 5 : Human tonsil lysates
Developed using the ECL technique. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD9 antibody (ab121663)ab121663, at 1/30 dilution, staining SAMD9 in paraffin-embedded Human thyroid gland tissue by Immunohistochemistry. -
Immunocytochemistry/ Immunofluorescence - Anti-SAMD9 antibody (ab121663)ab121663 at 4 µg/ml staining SAMD9 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab121663 尚未被引用在任何文献中。