Anti-SAMD12抗体(ab121831)
Key features and details
- Rabbit polyclonal to SAMD12
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SAMD12抗体 -
描述
兔多克隆抗体to SAMD12 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence: LHCGLNPRGI DHPAHAEGIK LQIEGEGVES QSIKNKNFQK VPDQKGTPKR LQAEAETAKS ATVKLSKPVA LWTQQDVCKW LKKHCPNQYQ IYSESF, corresponding to amino acids 6-101 of Human SAMD12 (UniProt ID:Q8N8I0).
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阳性对照
- Human brain tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121831于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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序列相似性
Contains 1 SAM (sterile alpha motif) domain. - Information by UniProt
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数据库链接
- Entrez Gene: 401474 Human
- SwissProt: Q8N8I0 Human
- Unigene: 359393 Human
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别名
- SAM domain-containing protein 12 antibody
- SAM12_HUMAN antibody
- Samd12 antibody
- Sterile alpha motif domain-containing protein 12 antibody
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD12 antibody (ab121831)
Immunohistochemical analysis of human cerebral cortex tissue labelling SAMD12 using ab121831 at a 1/200 dilution. Cytoplasmic staining observed in human neuronal cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD12 antibody (ab121831)Immunohistochemical analysis of human pancreas tissue labelling SAMD12 using ab121831 at a 1/200 dilution. Cytoplasmic staining observed in human islets of Langerhans.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD12 antibody (ab121831)Immunohistochemical analysis of human fallopian tube tissue labelling SAMD12 using ab121831 at a 1/200 dilution. Cytoplasmic staining observed in human glandular cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD12 antibody (ab121831)Immunohistochemical analysis of human colon tissue labelling SAMD12 using ab121831 at a 1/200 dilution. Cytoplasmic staining observed in human glandular cells.
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Immunocytochemistry/ Immunofluorescence - Anti-SAMD12 antibody (ab121831)Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm and golgi apparatus. Recommended concentration of ab121831 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Western blot - Anti-SAMD12 antibody (ab121831)All lanes : Anti-SAMD12 antibody (ab121831) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over expression Lysate (co-expressed with a C terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab121831 尚未被引用在任何文献中。