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AB8274

Anti-Salmonella typhimurium LPS 抗体 [1E6]

Anti-Salmonella typhimurium LPS antibody [1E6]

5

(2 Reviews)

|

(33 Publications)

Anti-Salmonella typhimurium LPS antibody [1E6] (ab8274) is a mouse monoclonal antibody detecting Salmonella typhimurium LPS in Western Blot, Flow Cytometry, ICC/IF, ELISA, EM, Dot Blot. Suitable for Salmonella typhimurium.

- Over 20 publications
- Trusted since 2002

查看别名

S. typhimurium, SALMONELLA TYPHIMURIUM LPS FACTOR 4, Salmonella typhimurium

2 Images
Western blot - Anti-Salmonella typhimurium LPS antibody [1E6] (AB8274)
  • WB

AbReview27910****

Western blot - Anti-Salmonella typhimurium LPS antibody [1E6] (AB8274)

Gels were (A) silver-stained or (B) Western blotted using the Anti-Salmonella typhimurium 0-4 antibody [1E6] (ab8274) as the primary antibody. Although equal amounts of cellular material were detected by silver staining, only the S. Typhimurium samples were recognized by the 1E6 antibody on the corresponding Western blot.

All lanes:

Western blot - Anti-Salmonella typhimurium LPS antibody [1E6] (ab8274) at 1/1000 dilution

Lane 1:

Whole cell polysaccharides from S. Enteritidis 27655-3b at 100000000 Cells

Lane 2:

Whole cell polysaccharides from S. Enteritidis Sal8 at 100000000 Cells

Lane 3:

Whole cell polysaccharides from S. Typhimurium ATCC 14028s at 100000000 Cells

Lane 4:

Whole cell polysaccharides from S. Typhimurium SL1344 at 100000000 Cells

Secondary

All lanes:

AP conjugated goat anti-mouse polyclonal at 1/4000 dilution

false

Image courtesy of Aaron White by Abreview.

Electron Microscopy - Anti-Salmonella typhimurium LPS antibody [1E6] (AB8274)
  • EM

AbReview7482****

Electron Microscopy - Anti-Salmonella typhimurium LPS antibody [1E6] (AB8274)

Electron microscopy image of ultrathin cryosections of Salmonella paratyphi using ab8274 (1/100). For further experimental details please refer to abreview.

This image is courtesy of an anonymous Abreview

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

1E6

亚型

IgG1

轻链类型

unknown

不含载体蛋白

No

反应种属

Salmonella typhimurium

应用

EM, Flow Cyt, ELISA, Dot, ICC/IF, WB

applications

特异性

This antibody is directed against lipopolisaccharides of Salmonella typhimurium

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反应性数据

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产品详情

What is this antibody validated in?
Anti-Salmonella typhimurium LPS antibody [1E6] (ab8274) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA, Electron microscopy (EM), Dot Blot in Salmonella typhimurium samples.

Trusted by the scientific community
Anti-Salmonella typhimurium LPS [1E6] (ab8274) was first used in a scientific publication in 2002 and has been cited over 20 times in peer-reviewed journals.
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Preservative: 0.1% Sodium azide
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Salmonella typhimurium LPS also known as lipopolysaccharide forms a vital component of the outer membrane of Gram-negative bacteria such as Salmonella. This complex molecule consists of a lipid A domain a core oligosaccharide and an O-antigen polysaccharide chain. The lipid A component with an estimated mass of roughly 10 kDa is the main contributor to LPS endotoxin activity. In Salmonella the LPS is expressed in the bacterial cell wall providing structural integrity and defense against environmental stresses. Its composition varies among different Salmonella strains influencing the bacterium's virulence and interaction with host immune systems.
Biological function summary

Salmonella typhimurium LPS engages in stimulating immune responses. An important part of the LPS structure the lipid A moiety activates the innate immune system by binding to Toll-like receptor 4 (TLR4) on host immune cells. This interaction triggers a cascade of pro-inflammatory cytokine production. As part of the outer membrane of Salmonella LPS works in synergy with other membrane proteins to form the protective barrier for the bacteria. The variability in the O-antigen portion aids in evasion of host immune responses allowing different Salmonella serotypes to survive and cause infection.

Pathways

The response of TLR4 to Salmonella typhimurium LPS forms a central axis in innate immune signaling. This pathway activates NF-kB a transcription factor pivotal in inducing cytokine genes thereby intensifying the antibacterial response. LPS interactions not only involve TLR4 but also influence pathways such as the MAPK signaling cascade further regulating immune responses. These pathways ensure rapid immune mobilization to control bacterial infections highlighting the significant role LPS plays in pathogen-host interactions.

Salmonella typhimurium LPS remains an important factor in the pathogenesis of salmonellosis and septic shock. LPS as a potent endotoxin contributes to systemic inflammation during septicemia by over-activating the immune system. Its interaction with TLR4 leads to high levels of inflammatory cytokines resulting in symptoms associated with both intestinal and systemic infections. The molecular attributes of LPS particularly lipid A connect to disease outcomes and also offer potential targets for therapeutic intervention in controlling severe inflammatory responses during Salmonella infections.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

See full target information Salmonella typhimurium LPS
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文献 (33)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2411233 PubMed39807570

2025

A Multicellular In Vitro Model of the Human Intestine with Immunocompetent Features Highlights Host-Pathogen Interactions During Early Salmonella Typhimurium Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Spyridon Damigos,Aylin Caliskan,Gisela Wajant,Sara Giddins,Adriana Moldovan,Sabine Kuhn,Evelyn Putz,Thomas Dandekar,Thomas Rudel,Alexander J Westermann,Daniela Zdzieblo

Antioxidants (Basel, Switzerland) 13: PubMed39456424

2024

Esculetin Combats Multidrug-Resistant Infection and Ameliorates Intestinal Dysfunction via the Nrf2 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjiao Xu,Wenjun Ding,Liyan Jia,Kui Zhu,Qingfeng Luo

Molecular therapy oncolytics 31:100745 PubMed38053546

2023

Bacterial cancer therapy using the attenuated fowl-adapted serovar Gallinarum.

Applications

Unspecified application

Species

Unspecified reactive species

Daejin Lim,Kwangsoo Kim,Taner Duysak,EunA So,Jae-Ho Jeong,Hyon E Choy

Microbiology spectrum 11:e0225323 PubMed37796020

2023

Lactate promotes intracellular replication and systemic infection via driving macrophage M2 polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyue Wang,Bin Yang,Shuangshuang Ma,Xiaolin Yan,Shuai Ma,Hongmin Sun,Yuyang Sun,Lingyan Jiang

mBio 13:e0037422 PubMed35862803

2022

Genetic and Structural Variation in the O-Antigen of Salmonella enterica Serovar Typhimurium Isolates Causing Bloodstream Infections in the Democratic Republic of the Congo.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Van Puyvelde,Gianmarco Gasperini,Michael Biggel,Marie-France Phoba,Maria Michelina Raso,Tessa de Block,Leen N Vanheer,Stijn Deborggraeve,Olivier Vandenberg,Nicholas Thomson,Neil Ravenscroft,Calman A Maclennan,Barbara Bellich,Paola Cescutti,Gordon Dougan,Jan Jacobs,Octavie Lunguya,Francesca Micoli

Journal of extracellular vesicles 11:e12192 PubMed35289114

2022

A bacterial extracellular vesicle-based intranasal vaccine against SARS-CoV-2 protects against disease and elicits neutralizing antibodies to wild-type and Delta variants.

Applications

Unspecified application

Species

Unspecified reactive species

Linglei Jiang,Tom A P Driedonks,Wouter S P Jong,Santosh Dhakal,H Bart van den Berg van Saparoea,Ioannis Sitaras,Ruifeng Zhou,Christopher Caputo,Kirsten Littlefield,Maggie Lowman,Mengfei Chen,Gabriela Lima,Olesia Gololobova,Barbara Smith,Vasiliki Mahairaki,M Riley Richardson,Kathleen R Mulka,Andrew P Lane,Sabra L Klein,Andrew Pekosz,Cory Brayton,Joseph L Mankowski,Joen Luirink,Jason S Villano,Kenneth W Witwer

Methods in molecular biology (Clifton, N.J.) 2414:227-279 PubMed34784041

2021

Methods for Assessment of OMV/GMMA Quality and Stability.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Micoli,Renzo Alfini,Carlo Giannelli

Science advances 7:eabj2485 PubMed34597140

2021

GABARAP sequesters the FLCN-FNIP tumor suppressor complex to couple autophagy with lysosomal biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan M Goodwin,Ward G Walkup,Kirsty Hooper,Taoyingnan Li,Chieko Kishi-Itakura,Aylwin Ng,Timothy Lehmberg,Archana Jha,Sravya Kommineni,Katherine Fletcher,Jorge Garcia-Fortanet,Yaya Fan,Qing Tang,Menghao Wei,Asmita Agrawal,Sagar R Budhe,Sreekanth R Rouduri,Dan Baird,Jeff Saunders,Janna Kiselar,Mark R Chance,Andrea Ballabio,Brent A Appleton,John H Brumell,Oliver Florey,Leon O Murphy

Nature communications 12:879 PubMed33563986

2021

Salmonella Typhimurium reprograms macrophage metabolism via T3SS effector SopE2 to promote intracellular replication and virulence.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyan Jiang,Peisheng Wang,Xiaorui Song,Huan Zhang,Shuangshuang Ma,Jingting Wang,Wanwu Li,Runxia Lv,Xiaoqian Liu,Shuai Ma,Jiaqi Yan,Haiyan Zhou,Di Huang,Zhihui Cheng,Chen Yang,Lu Feng,Lei Wang

Heliyon 7:e05903 PubMed33553721

2021

Solar inactivated Typhimurium induces an immune response in BALB/c mice.

Applications

Unspecified application

Species

Unspecified reactive species

Cornelius C Ssemakalu,Marta Ulaszewska,Sean Elias,Alexandra J Spencer
View all publications
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