Anti-SALL1抗体(ab31526)
Key features and details
- Rabbit polyclonal to SALL1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-SALL1抗体
参阅全部 SALL1 一抗 -
描述
兔多克隆抗体to SALL1 -
宿主
Rabbit -
特异性
Replenishment batches of ab31526 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Abpromise guarantee.
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经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse
预测可用于: Chicken, Human, Drosophila melanogaster
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 1300 to the C-terminus of Human SALL1.
参阅Abcam的专有抗源政策 (Peptide available as ab31903.) -
阳性对照
- ICC/IF: mES cells. WB: E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab31526于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
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| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 140 kDa).
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| 说明 |
|---|
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ICC/IF
Use a concentration of 1 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 140 kDa). |
靶标
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功能
Transcriptional repressor involved in organogenesis. -
组织特异性
Highest levels in kidney. Lower levels in adult brain (enriched in corpus callosum, lower expression in substantia nigra) and liver. -
疾病相关
Defects in SALL1 are the cause of Townes-Brocks syndrome (TBS) [MIM:107480]. TBS is a rare, autosomal dominant malformation syndrome with a combination of imperforate anus, triphalangeal and supernumerary thumbs, malformed ears and sensorineural hearing loss.
Defects in SALL1 may cause a phenotype overlapping with TBS, similar to bronchio-oto-renal syndrome (BOR) [MIM:113650]. BOR is an autosomal dominant disorder, manifested by various combinations of preauricular pits, branchial fistulae or cysts, lacrimal duct stenosis, hearing loss, structural defects of the outer, middle, or inner ear, and renal dysplasia. Associated defects include asthenic habitus, long narrow facies, constricted palate, deep overbite, and myopia. Hearing loss may be due to Mondini type cochlear defect and stapes fixation. -
序列相似性
Belongs to the sal C2H2-type zinc-finger protein family.
Contains 9 C2H2-type zinc fingers. -
发展阶段
In fetal brain exclusively in neurons of the subependymal region of hypothalamus lateral to the third ventricle. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 395446 Chicken
- Entrez Gene: 6299 Human
- Entrez Gene: 58198 Mouse
- Omim: 602218 Human
- SwissProt: Q9NSC2 Human
- SwissProt: Q9ER74 Mouse
- Unigene: 135787 Human
- Unigene: 214361 Mouse
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别名
- HSal1 antibody
- Sal like protein 1 antibody
- Sal-1 antibody
see all
图片
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Western blot - Anti-SALL1 antibody (ab31526)Anti-SALL1 antibody (ab31526) at 1 µg/ml + Mouse embryonic stem cell lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 140 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesBlocking buffer: 2% BSA block
Gel type: TA
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Immunocytochemistry/ Immunofluorescence - Anti-SALL1 antibody (ab31526)ab31526 staining SALL1 in mES cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab31526 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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Western blot - Anti-SALL1 antibody (ab31526)Anti-SALL1 antibody (ab31526) at 1 µg/ml + E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 140 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutesAlthough the predicted band size is 140 kDa based on Swiss-prot data, a band of 200 kDa has been previously observed. J Biol Chem. 2011 Jan 14;286(2):1037-45 PMID:21062744
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab31526 被引用在 16 文献中.
- Li Z et al. miR-4286 promotes prostate cancer progression by targeting the expression of SALL1. J Gene Med 25:e3127 (2023). PubMed: 31693770
- Lavicky J et al. The Development of Dentin Microstructure Is Controlled by the Type of Adjacent Epithelium. J Bone Miner Res 37:323-339 (2022). PubMed: 34783080
- Scott EP et al. The zinc finger transcription factor Sall1 is required for the early developmental transition of microglia in mouse embryos. Glia 70:1720-1733 (2022). PubMed: 35567352
- Errede M et al. Microglia-derived CCL2 has a prime role in neocortex neuroinflammation. Fluids Barriers CNS 19:68 (2022). PubMed: 36042496
- Krivanek J et al. Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth. Nat Commun 11:4816 (2020). PubMed: 32968047