Anti-Salivary alpha amylase抗体(ab125230)

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To answer the first of the customer's question: yes, both antibodies should bind against the active, native protein.


The difference between the two methods/ the two purification gradesis the following:


Affinity purification with immobilized antigen: Covalentlyimmobilizing purified antigen (i.e., the peptide or hapten used as the immunogen to induce production of antibody by the host animal) to an affinity support allows the specific antibody to be purified from crude samples.

The isolation of the immunoglobulin G (IgG) fraction (containing antibodies of all specificities) from a complex protein mixture such as antiserum, ascites fluid, or hybridoma supernatant. The basic procedure utilizes saturated ammonium sulfate solution to precipitate the IgG fraction, while an alternate protocol describes fractionation of IgG by chromatography.