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AB106096

Anti-SAE1 (phospho S185)抗体

Anti-SAE1 (phospho S185) antibody

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(1 Publication)

Rabbit Polyclonal SAE1 phospho S185 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SAE1 pS185.

查看别名

AOS1, SUA1, UBLE1A, SAE1, SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A

1 Images
Western blot - Anti-SAE1 (phospho S185) antibody (AB106096)
  • WB

Unknown

Western blot - Anti-SAE1 (phospho S185) antibody (AB106096)

10% SDS-PAGE. Lower panel shows tubulin loading control

All lanes:

Western blot - Anti-SAE1 (phospho S185) antibody (ab106096) at 1/1000 dilution

Lane 1:

HeLa whole cell protein at 20 µg

Lane 2:

HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail at 20 µg

Secondary

All lanes:

HRP-conjugated secondary

Predicted band size: 38 kDa

true

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB

applications

免疫原

Synthetic Peptide within Human SAE1 pS185. The exact immunogen used to generate this antibody is proprietary information.

Q9UBE0

特异性

<p>This purified antibody is directed against human SUMO Activating Enzyme E1 protein. A BLAST analysis using the sequence of the immunizing peptide was used to suggest that this antibody would react with SUMO Activating Enzyme E1 protein from human (100%), bovine, dog, chimpanzee (96%), mouse (93%), and rat (92%) based on a high degree of sequence homology. Cross reactivity against this protein from other sources has not been determined.</p>

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
纯化说明
This purified antibody is directed against human SUMO Activating Enzyme E1 protein. The product was purified from monospecific antiserum by affinity chromatography.
存储溶液
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The protein SAE1 also known as SUMO-activating enzyme subunit 1 functions mechanically as a part of the SUMOylation process. It has a mass of approximately 39 kDa and is expressed in multiple cell types but is mainly found in the nucleus. SAE1 forms a heterodimeric structure with the protein SAE2 and together they are responsible for the initial step of SUMO activation which is important for SUMOylation a post-translational modification process.
Biological function summary

SAE1 is integral to numerous cellular processes that require precise regulation such as transcription DNA repair and signal transduction. It functions as part of the SUMO E1 enzyme complex which is essential for conjugating SUMO proteins to target substrates. This modification influences protein stability localization and interaction adding a layer of control in these cellular activities. The modulation of protein function by SAE1 impacts cell cycle control and stress responses indicating its breadth of influence across cellular mechanisms.

Pathways

The actions of SAE1 significantly impact the PI3K-Akt and DNA damage repair pathways. Its involvement in these pathways highlights its role in regulating cell survival and maintaining genomic integrity. SAE1's interaction within the SUMOylation machinery connects it to other proteins such as Ubc9 the E2 conjugating enzyme which highlights its importance in facilitating modifications necessary for a wide array of cellular functions and signaling cascades.

Defects or dysregulation of SAE1 have been linked to cancer and neurodegenerative conditions. Altered SUMOylation levels controlled by SAE1 can influence tumorigenesis through abnormal cell proliferation or impaired DNA damage responses connecting it with proteins like p53 and PARP1 which are critical in those pathways. Additionally SAE1's involvement in neurological disorders is associated with disrupted protein homeostasis linking it to pathologies where proper cellular regulatory mechanisms are compromised.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
See full target information SAE1 pS185
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e22336 PubMed35522243

2022

Canonical and noncanonical regulatory roles for JAK2 in the pathogenesis of rheumatoid arthritis-associated interstitial lung disease and idiopathic pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Shaohua Wang,Mengfei Liu,Xiujuan Li,Jie Zhang,Faping Wang,Chujie Zhang,Anja Roden,Jay H Ryu,Kenneth J Warrington,Jie Sun,Eric L Matteson,Daniel J Tschumperlin,Robert Vassallo
View all publications
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