Anti-SA2抗体(ab4463)
Key features and details
- Goat polyclonal to SA2
- Suitable for: IP, WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-SA2抗体
参阅全部 SA2 一抗 -
描述
山羊多克隆抗体to SA2 -
宿主
Goat -
特异性
We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
经测试应用
适用于: IP, WB, ICCmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human SA2 aa 1100-1200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_006594.3 (GeneID 10735).
Database link: Q8N3U4 -
阳性对照
- WB: HeLa nuclear cell extract. HeLa, HEK-293T and NIH/3T3 whole cell lysate. ICC: HeLa cells. IP: SA2 IP in HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
|
|
| WB |
1/1000 - 1/10000. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).
|
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| ICC |
1/500 - 1/2000.
|
| 说明 |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa). |
|
ICC
1/500 - 1/2000. |
靶标
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功能
Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. -
序列相似性
Belongs to the SCC3 family.
Contains 1 SCD (stromalin conservative) domain. -
翻译后修饰
Phosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation. -
细胞定位
Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. - Information by UniProt
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数据库链接
- Entrez Gene: 10735 Human
- Entrez Gene: 20843 Mouse
- Omim: 300826 Human
- SwissProt: Q8N3U4 Human
- SwissProt: O35638 Mouse
- Unigene: 496710 Human
- Unigene: 624663 Human
- Unigene: 290422 Mouse
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别名
- bA517O1.1 antibody
- Cohesin Subunit SA 2 antibody
- Cohesin subunit SA-2 antibody
see all
图片
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Western blot - Anti-SA2 antibody (ab4463)
Whole cell lysate (50 µg) from HeLa, HEK-293T, and NIH/3T3 cells prepared using NETN lysis buffer.
ab4463 used for WB at 0.1 µg/ml.
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Immunoprecipitation - Anti-SA2 antibody (ab4463)Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Lane 1: ab4463 used for IP at 6 µg per reaction.
Lane 2: Control IgG.
For blotting immunoprecipitated SA2, ab4463 was used at 1 µg/ml.
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Western blot - Anti-SA2 antibody (ab4463)Nuclear Extract from HeLa cells (~5 x 105 cells).
ab4463 at 0.5
µ g/ml.Detection: ECL with 15 second exposure.
Nuclear Extract from HeLa cells (~5 x 10e5 cells).
ab4463 at 0.5 µg/ml.
Detection: ECL with 15 second exposure. -
Immunocytochemistry - Anti-SA2 antibody (ab4463)Immunocytochemistry analysis of HeLa cells (extracted for 5 minutes at 4°C in 0.5% Triton in CSK buffer) labelling SA2 with ab4463 at 1µg/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab4463 被引用在 9 文献中.
- Liu G et al. CDK6 is stimulated by hyperthermia and protects gastric cancer cells from hyperthermia-induced damage. Mol Med Rep 23:N/A (2021). PubMed: 33537819
- Surdez D et al. STAG2 mutations alter CTCF-anchored loop extrusion, reduce cis-regulatory interactions and EWSR1-FLI1 activity in Ewing sarcoma. Cancer Cell 39:810-826.e9 (2021). PubMed: 33930311
- Meisenberg C et al. Repression of Transcription at DNA Breaks Requires Cohesin throughout Interphase and Prevents Genome Instability. Mol Cell 73:212-223.e7 (2019). PubMed: 30554942
- Fukuda T et al. STAG3-mediated stabilization of REC8 cohesin complexes promotes chromosome synapsis during meiosis. EMBO J 33:1243-55 (2014). PubMed: 24797475
- Ishiguro K et al. A new meiosis-specific cohesin complex implicated in the cohesin code for homologous pairing. EMBO Rep 12:267-75 (2011). WB ; Mouse . PubMed: 21274006
- Xiao T et al. Specific Sites in the C Terminus of CTCF Interact with the SA2 Subunit of the Cohesin Complex and Are Required for Cohesin-Dependent Insulation Activity. Mol Cell Biol 31:2174-83 (2011). WB ; Human . PubMed: 21444719
- Tanno Y et al. Phosphorylation of mammalian Sgo2 by Aurora B recruits PP2A and MCAK to centromeres. Genes Dev 24:2169-79 (2010). WB ; Human . PubMed: 20889715
- Yao H et al. Mediation of CTCF transcriptional insulation by DEAD-box RNA-binding protein p68 and steroid receptor RNA activator SRA. Genes Dev 24:2543-55 (2010). WB ; Human . PubMed: 20966046
- Sun Y et al. Separase is recruited to mitotic chromosomes to dissolve sister chromatid cohesion in a DNA-dependent manner. Cell 137:123-32 (2009). WB ; Human . PubMed: 19345191