SA1重组抗体[EPR26227-76]_SA1抗体(ab317301)| Abcam中文官网

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Western blot - Anti-SA1 antibody [EPR26227-76] (ab317301)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR26227-76] to SA1
Suitable for: Flow Cyt (Intra), ICC/IF, IP, ChIP-sequencing, WB
Reacts with: Mouse, Rat, Human

Carrier Free

产品名称

Anti-SA1抗体[EPR26227-76]
参阅全部 SA1 一抗
描述

兔单克隆抗体[EPR26227-76] to SA1
宿主

Rabbit
特异性

Unsuitable for mouse ICC. We recommend using fresh lysate as SA1 can undergo degradation easily. We have observed loss of signal when using frozen lysate.
经测试应用

适用于: Flow Cyt (Intra), ICC/IF, IP, ChIP-sequencing, WBmore details
不适用于: IHC-P
种属反应性

与反应: Mouse, Rat, Human
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: HeLa, 293T, NIH/3T3, PC-12, MCF7 and His-tagged Human STAG1 protein. ICC/IF: HeLa cells. Flow Cyt (Intra): HeLa and NIH/3T3 cells. IP: HeLa and NIH/3T3 whole cell lysate.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
存储溶液

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR26227-76
同种型

IgG
研究领域

Alternative Versions
- Anti-SA1 antibody [EPR26227-76] - BSA and Azide free (ab317302)
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products

The Abpromise guarantee

Abpromise™承诺保证使用ab317301于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
Flow Cyt (Intra)		1/50.
ICC/IF		1/200.
IP		1/30.
ChIP-sequencing		Use 8µg for 10⁷ cells.
WB		1/1000.

说明
Flow Cyt (Intra) 1/50.
ICC/IF 1/200.
IP 1/30.
ChIP-sequencing Use 8µg for 10⁷ cells.
WB 1/1000.

应用说明

Is unsuitable for IHC-P.

功能

Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
序列相似性

Belongs to the SCC3 family.
Contains 1 SCD (stromalin conservative) domain.
翻译后修饰

Phosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation.
细胞定位

Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.
Target information above from: UniProt accession Q8WVM7 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 10274 Human
- Entrez Gene: 20842 Mouse
- Entrez Gene: 315958 Rat
- Omim: 604358 Human
- SwissProt: Q8WVM7 Human
- SwissProt: Q9D3E6 Mouse
- Unigene: 412586 Human
- Unigene: 42135 Mouse
别名
- Cohesin subunit SA 1 antibody
- Cohesin subunit SA-1 antibody
- Cohesin Subunit SA1 antibody
- Nuclear protein stromal antigen 1 antibody
- SA 1 antibody
- SCC 3 homolog 1 antibody
- Scc3 antibody
- SCC3 homolog 1 antibody
- STAG 1 antibody
- stag1 antibody
- STAG1_HUMAN antibody
- Stromal antigen 1 antibody
see all

Western blot - Anti-SA1 antibody [EPR26227-76] (ab317301)

All lanes : Anti-SA1 antibody [EPR26227-76] (ab317301) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate
Lane 2 : HeLa transfected with siRNA specifically targeting SA1 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 145 kDa why is the actual band size different from the predicted?

Exposure time: 180 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Western blot - Anti-SA1 antibody [EPR26227-76] (ab317301)

All lanes : Anti-SA1 antibody [EPR26227-76] (ab317301) at 1/1000 dilution

Lane 1 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 145 kDa why is the actual band size different from the predicted?

Exposure time: 180 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The blot lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

SA1 can undergo degradation easily.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Western blot - Anti-SA1 antibody [EPR26227-76] (ab317301)

All lanes : Anti-SA1 antibody [EPR26227-76] (ab317301) at 1/1000 dilution

Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : MCF7 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 145 kDa why is the actual band size different from the predicted?

Exposure time: 180 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Lane 1 lysate was freshly made and used for Western Blotting immediately to minimize protein degradation.

SA1 can undergo degradation easily.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Western blot - Anti-SA1 antibody [EPR26227-76] (ab317301)

All lanes : Anti-SA1 antibody [EPR26227-76] (ab317301) at 1/1000 dilution

Lane 1 : His-tagged Human STAG1 protein
Lane 2 : His-tagged Human STAG2 protein

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 19,38 kDa why is the actual band size different from the predicted?

Exposure time: 180 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

This antibody does not cross-react with human STAG2.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-SA1 antibody [EPR26227-76] (ab317301)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling SA1 with ab317301 at 1/200 (10.34 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing mainly nuclear staining in HeLa cell line (shown in green). The counterstain was observed in red. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Flow Cytometry (Intracellular) - Anti-SA1 antibody [EPR26227-76] (ab317301)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling SA1 with ab317301 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-SA1 antibody [EPR26227-76] (ab317301)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling SA1 with ab317301 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-SA1 antibody [EPR26227-76] (ab317301)

SA1 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab317301 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317301 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2: ab317301 IP in HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317301 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 41 seconds.
Immunoprecipitation - Anti-SA1 antibody [EPR26227-76] (ab317301)

SA1 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab317301 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317301 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 2: ab317301 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317301 in NIH/3T3 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 154 seconds.
ChIP-sequencing - Anti-SA1 antibody [EPR26227-76] (ab317301)

Chromatin was prepared from MCF7 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of ab317301 [EPR26227-76]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.
ChIP-sequencing - Anti-SA1 antibody [EPR26227-76] (ab317301)

Chromatin was prepared from MCF7 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of ab317301 [EPR26227-76]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.
ChIP-sequencing - Anti-SA1 antibody [EPR26227-76] (ab317301)

Chromatin was prepared from MCF7 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of ab317301 [EPR26227-76]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.
Anti-SA1 antibody [EPR26227-76] (ab317301)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

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Key features and details

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概述

产品名称

描述

宿主

特异性

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

Related Products

应用

The Abpromise guarantee

靶标

功能

序列相似性

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

Certificate of Compliance

文献 (0)

客户评价及客户问答