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Anti-SA1抗体(ab4457)

  • Datasheet
  • SDS
Submit a review Q&A (3)References (11)

Product price, shipping and contact information

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Western blot - Anti-SA1 antibody (ab4457)
  • Immunoprecipitation - Anti-SA1 antibody (ab4457)
  • Immunocytochemistry - Anti-SA1 antibody (ab4457)

Key features and details

  • Goat polyclonal to SA1
  • Suitable for: ChIP, WB, ICC, IP, ChIP/Chip
  • Reacts with: Mouse, Human, Xenopus laevis
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-SA1 antibody [SUSI63B] (ab241544)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-SA1抗体
    参阅全部 SA1 一抗
  • 描述

    山羊多克隆抗体to SA1
  • 宿主

    Goat
  • 经测试应用

    适用于: ChIP, WB, ICC, IP, ChIP/Chipmore details
  • 种属反应性

    与反应: Mouse, Human, Xenopus laevis
    预测可用于: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey
  • 免疫原

    Synthetic peptide corresponding to Human SA1 aa 1150-1200.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Chromatid Cohesion
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Chromosome Structure
    • Chromatid Cohesion

相关产品

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab4457于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ChIP
Use at an assay dependent concentration. PubMed: 18550811
WB
1/2000 - 1/10000. Detects a band of approximately 144 kDa (predicted molecular weight: 144 kDa).
ICC
1/500 - 1/2000.
IP
Use at 2-10 µg/mg of lysate.
ChIP/Chip
Use at an assay dependent concentration. PubMed: 18550811
说明
ChIP
Use at an assay dependent concentration. PubMed: 18550811
WB
1/2000 - 1/10000. Detects a band of approximately 144 kDa (predicted molecular weight: 144 kDa).
ICC
1/500 - 1/2000.
IP
Use at 2-10 µg/mg of lysate.
ChIP/Chip
Use at an assay dependent concentration. PubMed: 18550811

靶标

  • 功能

    Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
  • 序列相似性

    Belongs to the SCC3 family.
    Contains 1 SCD (stromalin conservative) domain.
  • 翻译后修饰

    Phosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation.
  • 细胞定位

    Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.
  • Target information above from: UniProt accession Q8WVM7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 10274 Human
    • Entrez Gene: 20842 Mouse
    • Entrez Gene: 399163 Xenopus laevis
    • Omim: 604358 Human
    • SwissProt: Q8WVM7 Human
    • SwissProt: Q9D3E6 Mouse
    • SwissProt: Q9DGN1 Xenopus laevis
    • Unigene: 412586 Human
    • Unigene: 42135 Mouse
    • Unigene: 3441 Xenopus laevis
    see all
  • 别名

    • Cohesin subunit SA 1 antibody
    • Cohesin subunit SA-1 antibody
    • Cohesin Subunit SA1 antibody
    • Nuclear protein stromal antigen 1 antibody
    • SA 1 antibody
    • SCC 3 homolog 1 antibody
    • Scc3 antibody
    • SCC3 homolog 1 antibody
    • STAG 1 antibody
    • stag1 antibody
    • STAG1_HUMAN antibody
    • Stromal antigen 1 antibody
    see all

图片

  • Western blot - Anti-SA1 antibody (ab4457)
    Western blot - Anti-SA1 antibody (ab4457)
    All lanes : Anti-SA1 antibody (ab4457) at 1/10000 dilution

    Lane 1 : HeLa Whole cell lysate
    Lane 2 : 293T Whole cell lysate
    Lane 3 : Jurkat Whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 144 kDa



    Detection: Chemiluminescence with an exposure time of 30 seconds.

  • Immunoprecipitation - Anti-SA1 antibody (ab4457)
    Immunoprecipitation - Anti-SA1 antibody (ab4457)

    SA1 was immunoprecipitated from HeLa whole cell lysate with 6 µg/mg lysate ab4457. Western blot was performed from the immunoprecipitate using ab4457 at 0.1 µg/ml dilution.

    Lane 1: ab4457 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

  • Immunocytochemistry - Anti-SA1 antibody (ab4457)
    Immunocytochemistry - Anti-SA1 antibody (ab4457)
    Immunocytochemistry analysis of HeLa cells (extracted for 5 minutes at 4°C in 0.5% Triton in CSK buffer) labelling SA1 with ab4457 at 1µg/ml.

实验方案

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (11)

发表研究结果有使用 ab4457?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab4457 被引用在 11 文献中.

  • van der Weide RH  et al. Hi-C analyses with GENOVA: a case study with cohesin variants. NAR Genom Bioinform 3:lqab040 (2021). PubMed: 34046591
  • Lovejoy CA  et al. ATRX affects the repair of telomeric DSBs by promoting cohesion and a DAXX-dependent activity. PLoS Biol 18:e3000594 (2020). PubMed: 31895940
  • Nora EP  et al. Molecular basis of CTCF binding polarity in genome folding. Nat Commun 11:5612 (2020). PubMed: 33154377
  • Meisenberg C  et al. Repression of Transcription at DNA Breaks Requires Cohesin throughout Interphase and Prevents Genome Instability. Mol Cell 73:212-223.e7 (2019). PubMed: 30554942
  • Steiner LA  et al. CTCF and CohesinSA-1 Mark Active Promoters and Boundaries of Repressive Chromatin Domains in Primary Human Erythroid Cells. PLoS One 11:e0155378 (2016). ChIP . PubMed: 27219007
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-3 of 3 Abreviews or Q&A

Question

It states that the immunogen used was a synthetic peptide (Human) - 35 amino acids which represented a portion of SA 1 encoded in part by exons 35 and 36. However, the customer says that there are only 34 exons. Please explain more about the immunogen and where the information is stating there are exons 35 and 36.

Read More

Abcam community

Verified customer

Asked on May 09 2006

Answer

The epitope recognized by ab4457 maps to a region between residues 1150 and 1200 of human Stromal Antigen 1 using the numbering given in entry NP_005853.2 (GeneID 10274). I hope this helps, please contact us again if you have any additional questions.

Read More

Abcam Scientific Support

回复于 May 10 2006

Question

Ok thanks. I would like to order as soon as possible but I am not sure how your company normally test the use of antibodies for ICC and how you can be sure that the signal you are getting is specific. So, the information would be of great help.

Read More

Abcam community

Verified customer

Asked on Jun 17 2004

Answer

Thank you very much for your patience. I'm afraid that I am unable to get this information from the originator of ab4455 and ab4457. I apologize for the inconvenience and if I can be of further assistance, please do let me know.

Read More

Abcam Scientific Support

回复于 Jul 07 2004

Question

I have a question related to the four types of goat anti-SCC3 antibodies that you have (Ab4455, Ab4457, Ab4463, and Ab4464). Do you know which one works best on immunostaining?

Read More

Abcam community

Verified customer

Asked on May 05 2004

Answer

Thank you for your email. All the information that we have regarding these antibodies is located on the online datasheets. I unfortunately can't tell you which one works "best." Are you detecting SA1 or SA2? The differences between the two SA1 antibodies and the two SA2 antibodies are the immunogens. The antibodies are fairly new and we have not had feedback from customers yet. If you have any more questions, please contact us again.

Read More

Abcam Scientific Support

回复于 May 06 2004

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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